Production of urokinase by HT 1080 human kidney cell line
Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and uroki...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Marcel Dekker
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50986/ http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf |
| _version_ | 1848851710257135616 |
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| author | Roychoudhury, Pradip A. Gomes, James Bhattacharyay, Sanjoy K. Abdulah, Norhafizah |
| author_facet | Roychoudhury, Pradip A. Gomes, James Bhattacharyay, Sanjoy K. Abdulah, Norhafizah |
| author_sort | Roychoudhury, Pradip A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated. |
| first_indexed | 2025-11-15T10:26:30Z |
| format | Article |
| id | upm-50986 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:26:30Z |
| publishDate | 1999 |
| publisher | Marcel Dekker |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-509862024-08-08T00:44:40Z http://psasir.upm.edu.my/id/eprint/50986/ Production of urokinase by HT 1080 human kidney cell line Roychoudhury, Pradip A. Gomes, James Bhattacharyay, Sanjoy K. Abdulah, Norhafizah Studies were carried out in T-flasks and bioreactor to produce urokinase enzyme using HT 1080 human kidney cell line. While growing the cell line it has been observed that the lag phase is reduced considerably in the bioreactor as compared to T-flask culture. The HT 1080 cell adhesion rate and urokinase production were observed to be the function of serum concentration in the medium. The maximum urokinase activity of 3.1 x 10-4 unit ml-1 was achieved in the bioreactor at around 65 h of batch culture. Since HT 1080 is an anchorage dependent cell line, therefore, the hydrodynamic effects on the cell line were investigated. Marcel Dekker 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf text en http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf Roychoudhury, Pradip A. and Gomes, James and Bhattacharyay, Sanjoy K. and Abdulah, Norhafizah (1999) Production of urokinase by HT 1080 human kidney cell line. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 27 (5&6). pp. 399-402. ISSN 1073-1199 http://www.tandfonline.com/doi/abs/10.3109/10731199909117710 10.3109/10731199909117710 |
| spellingShingle | Roychoudhury, Pradip A. Gomes, James Bhattacharyay, Sanjoy K. Abdulah, Norhafizah Production of urokinase by HT 1080 human kidney cell line |
| title | Production of urokinase by HT 1080 human kidney cell line |
| title_full | Production of urokinase by HT 1080 human kidney cell line |
| title_fullStr | Production of urokinase by HT 1080 human kidney cell line |
| title_full_unstemmed | Production of urokinase by HT 1080 human kidney cell line |
| title_short | Production of urokinase by HT 1080 human kidney cell line |
| title_sort | production of urokinase by ht 1080 human kidney cell line |
| url | http://psasir.upm.edu.my/id/eprint/50986/ http://psasir.upm.edu.my/id/eprint/50986/ http://psasir.upm.edu.my/id/eprint/50986/ http://psasir.upm.edu.my/id/eprint/50986/1/50986.pdf http://psasir.upm.edu.my/id/eprint/50986/7/Production%20of%20Urokinase%20by%20HT%201080%20Human%20Kidney%20cell%20line.pdf |