Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah

Algal have attracted attention from biomedical scientists as they are a valuable natural source of secondary metabolites that exhibit antioxidant activities. In this study, single-factor experiments were conducted to investigate the best extraction conditions (ethanol concentration, solid-to-solvent...

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Main Authors: Fu, Carmen Wai Foong, Ho, Chun Wai, Yong, Wilson Thau Lym, Abas, Faridah, Tan, Tai Boon, Tan, Chin Ping
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/50469/
http://psasir.upm.edu.my/id/eprint/50469/1/%288%29.pdf
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author Fu, Carmen Wai Foong
Ho, Chun Wai
Yong, Wilson Thau Lym
Abas, Faridah
Tan, Tai Boon
Tan, Chin Ping
author_facet Fu, Carmen Wai Foong
Ho, Chun Wai
Yong, Wilson Thau Lym
Abas, Faridah
Tan, Tai Boon
Tan, Chin Ping
author_sort Fu, Carmen Wai Foong
building UPM Institutional Repository
collection Online Access
description Algal have attracted attention from biomedical scientists as they are a valuable natural source of secondary metabolites that exhibit antioxidant activities. In this study, single-factor experiments were conducted to investigate the best extraction conditions (ethanol concentration, solid-to-solvent ratio, extraction temperature and extraction time) in extracting antioxidant compounds and capacities from four species of seaweeds (Sargassum polycystum, Eucheuma denticulatum, Kappaphycus alvarezzi variance Buaya and Kappaphycus alvarezzi variance Giant) from Sabah. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used to determine the phenolic and flavonoid concentrations, respectively, while 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picylhydrazyl (DPPH) radical scavenging capacity assays were used to evaluate the antioxidant capacities of all seaweed extracts. Results showed that extraction parameters had significant effect (p < 0.05) on the antioxidant compounds and antioxidant capacities of seaweed. Sargassum polycystum portrayed the most antioxidant compounds (37.41 ± 0.01 mg GAE/g DW and 4.54 ± 0.02 mg CE/g DW) and capacities (2.00 ± 0.01 μmol TEAC/g DW and 0.84 ± 0.01 μmol TEAC/g DW) amongst four species of seaweed.
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spelling upm-504692017-02-28T10:40:57Z http://psasir.upm.edu.my/id/eprint/50469/ Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah Fu, Carmen Wai Foong Ho, Chun Wai Yong, Wilson Thau Lym Abas, Faridah Tan, Tai Boon Tan, Chin Ping Algal have attracted attention from biomedical scientists as they are a valuable natural source of secondary metabolites that exhibit antioxidant activities. In this study, single-factor experiments were conducted to investigate the best extraction conditions (ethanol concentration, solid-to-solvent ratio, extraction temperature and extraction time) in extracting antioxidant compounds and capacities from four species of seaweeds (Sargassum polycystum, Eucheuma denticulatum, Kappaphycus alvarezzi variance Buaya and Kappaphycus alvarezzi variance Giant) from Sabah. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used to determine the phenolic and flavonoid concentrations, respectively, while 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picylhydrazyl (DPPH) radical scavenging capacity assays were used to evaluate the antioxidant capacities of all seaweed extracts. Results showed that extraction parameters had significant effect (p < 0.05) on the antioxidant compounds and antioxidant capacities of seaweed. Sargassum polycystum portrayed the most antioxidant compounds (37.41 ± 0.01 mg GAE/g DW and 4.54 ± 0.02 mg CE/g DW) and capacities (2.00 ± 0.01 μmol TEAC/g DW and 0.84 ± 0.01 μmol TEAC/g DW) amongst four species of seaweed. Faculty of Food Science and Technology, Universiti Putra Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50469/1/%288%29.pdf Fu, Carmen Wai Foong and Ho, Chun Wai and Yong, Wilson Thau Lym and Abas, Faridah and Tan, Tai Boon and Tan, Chin Ping (2016) Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah. International Food Research Journal, 23 (6). pp. 2363-2369. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/23%20(06)%202016/(8).pdf
spellingShingle Fu, Carmen Wai Foong
Ho, Chun Wai
Yong, Wilson Thau Lym
Abas, Faridah
Tan, Tai Boon
Tan, Chin Ping
Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title_full Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title_fullStr Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title_full_unstemmed Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title_short Extraction of phenolic antioxidants from four selected seaweeds obtained from Sabah
title_sort extraction of phenolic antioxidants from four selected seaweeds obtained from sabah
url http://psasir.upm.edu.my/id/eprint/50469/
http://psasir.upm.edu.my/id/eprint/50469/
http://psasir.upm.edu.my/id/eprint/50469/1/%288%29.pdf