The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intra-articular injection of a single dose of autologous chondrogenic induced BMSC could retard th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50208/ http://psasir.upm.edu.my/id/eprint/50208/1/The%20potential%20of%20intra-articular%20injection%20of%20chondrogenic-induced%20bone%20marrow%20stem%20cells%20to%20retard%20the%20progression%20of%20osteoarthritis%20in%20a%20sheep%20model.pdf |
| _version_ | 1848851490910765056 |
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| author | Al-Faqeh, Hamoud Hussien Mohammad Mohamad Yahaya, Nor Hamdan Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah |
| author_facet | Al-Faqeh, Hamoud Hussien Mohammad Mohamad Yahaya, Nor Hamdan Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah |
| author_sort | Al-Faqeh, Hamoud Hussien Mohammad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intra-articular injection of a single dose of autologous chondrogenic induced BMSC could retard the progressive destruction of cartilage in a surgically induced osteoarthritis in sheep. Sheep BMSCs were isolated and divided into two groups. One group was cultured in chondrogenic media containing (Ham's F12:DMEM, 1:1) FD + 1% FBS + 5 ng/ml TGFβ3 + 50 ng/ml IGF-1 (CM), and the other group was cultured in the basal media, FD + 10% FBS (BM). The procedure for surgically induced osteoarthritis was performed on the donor sheep 6 weeks prior to intra-articular injection into the knee joint of a single dose of BMSC from either group, suspended in 5 ml FD at density of 2 million cells/ml. The control groups were injected with basal media, without cells. Six weeks after injection, gross evidence of retardation of cartilage destruction was seen in the osteoarthritic knee joints treated with CM as well as BM. No significant ICRS (International Cartilage Repair Society) scoring was detected between the two groups with cells. However macroscopically, meniscus repair was observed in the knee joint treated with CM. Severe osteoarthritis and meniscal injury was observed in the control group. Interestingly, histologically the CM group demonstrated good cartilage histoarchitecture, thickness and quality, comparable to normal knee joint cartilage. As a conclusion, intra-articular injection of a single dose of BMSC either chondrogenically induced or not, could retard the progression of osteoarthritis (OA) in a sheep model, but the induced cells indicated better results especially in meniscus regeneration. |
| first_indexed | 2025-11-15T10:23:01Z |
| format | Article |
| id | upm-50208 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:23:01Z |
| publishDate | 2012 |
| publisher | Elsevier |
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| spelling | upm-502082017-02-02T05:01:00Z http://psasir.upm.edu.my/id/eprint/50208/ The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model Al-Faqeh, Hamoud Hussien Mohammad Mohamad Yahaya, Nor Hamdan Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intra-articular injection of a single dose of autologous chondrogenic induced BMSC could retard the progressive destruction of cartilage in a surgically induced osteoarthritis in sheep. Sheep BMSCs were isolated and divided into two groups. One group was cultured in chondrogenic media containing (Ham's F12:DMEM, 1:1) FD + 1% FBS + 5 ng/ml TGFβ3 + 50 ng/ml IGF-1 (CM), and the other group was cultured in the basal media, FD + 10% FBS (BM). The procedure for surgically induced osteoarthritis was performed on the donor sheep 6 weeks prior to intra-articular injection into the knee joint of a single dose of BMSC from either group, suspended in 5 ml FD at density of 2 million cells/ml. The control groups were injected with basal media, without cells. Six weeks after injection, gross evidence of retardation of cartilage destruction was seen in the osteoarthritic knee joints treated with CM as well as BM. No significant ICRS (International Cartilage Repair Society) scoring was detected between the two groups with cells. However macroscopically, meniscus repair was observed in the knee joint treated with CM. Severe osteoarthritis and meniscal injury was observed in the control group. Interestingly, histologically the CM group demonstrated good cartilage histoarchitecture, thickness and quality, comparable to normal knee joint cartilage. As a conclusion, intra-articular injection of a single dose of BMSC either chondrogenically induced or not, could retard the progression of osteoarthritis (OA) in a sheep model, but the induced cells indicated better results especially in meniscus regeneration. Elsevier 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50208/1/The%20potential%20of%20intra-articular%20injection%20of%20chondrogenic-induced%20bone%20marrow%20stem%20cells%20to%20retard%20the%20progression%20of%20osteoarthritis%20in%20a%20sheep%20model.pdf Al-Faqeh, Hamoud Hussien Mohammad and Mohamad Yahaya, Nor Hamdan and Chen, Hui Cheng and Saim, Aminuddin and Idrus, Ruszymah (2012) The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. Experimental Gerontology, 47 (6). pp. 458-464. ISSN 0531-5565; ESSN: 1873-6815 http://www.sciencedirect.com/science/article/pii/S0531556512000733 10.1016/j.exger.2012.03.018 |
| spellingShingle | Al-Faqeh, Hamoud Hussien Mohammad Mohamad Yahaya, Nor Hamdan Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title | The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title_full | The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title_fullStr | The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title_full_unstemmed | The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title_short | The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| title_sort | potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model |
| url | http://psasir.upm.edu.my/id/eprint/50208/ http://psasir.upm.edu.my/id/eprint/50208/ http://psasir.upm.edu.my/id/eprint/50208/ http://psasir.upm.edu.my/id/eprint/50208/1/The%20potential%20of%20intra-articular%20injection%20of%20chondrogenic-induced%20bone%20marrow%20stem%20cells%20to%20retard%20the%20progression%20of%20osteoarthritis%20in%20a%20sheep%20model.pdf |