Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography
Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). Th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/49976/ http://psasir.upm.edu.my/id/eprint/49976/1/Purification%20and%20characterization%20of%20very%20low%20density%20lipoprotein%20in%20commercial%20broiler%20and%20crossbred%20village%20chickens%20by%20fast%20protein%20liquid%20chromatography.pdf |
| _version_ | 1848851425224818688 |
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| author | Tan, Bee Koon Foo, Hooi Ling Loh, Teck Chwen Abdullah, Norhani Idrus, Zulkifli |
| author_facet | Tan, Bee Koon Foo, Hooi Ling Loh, Teck Chwen Abdullah, Norhani Idrus, Zulkifli |
| author_sort | Tan, Bee Koon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). The particle size of VLDL is 46.8 8.6 nm. The VLDL fraction was then subfractionated and the apolipoprotein (apo) profile was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). The CB and AK have almost similar types of apo in both subfractions 1 and 2. The AK showed the presence of apoAI, AIV, D and E whereas the CB had apo AIV, D, E and H. The apo AIV and apo E were present in both subfractions of AK and CB. |
| first_indexed | 2025-11-15T10:21:59Z |
| format | Article |
| id | upm-49976 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:21:59Z |
| publishDate | 2005 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-499762016-12-30T05:37:14Z http://psasir.upm.edu.my/id/eprint/49976/ Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography Tan, Bee Koon Foo, Hooi Ling Loh, Teck Chwen Abdullah, Norhani Idrus, Zulkifli Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). The particle size of VLDL is 46.8 8.6 nm. The VLDL fraction was then subfractionated and the apolipoprotein (apo) profile was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). The CB and AK have almost similar types of apo in both subfractions 1 and 2. The AK showed the presence of apoAI, AIV, D and E whereas the CB had apo AIV, D, E and H. The apo AIV and apo E were present in both subfractions of AK and CB. Asian-Australasian Association of Animal Production Societies 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/49976/1/Purification%20and%20characterization%20of%20very%20low%20density%20lipoprotein%20in%20commercial%20broiler%20and%20crossbred%20village%20chickens%20by%20fast%20protein%20liquid%20chromatography.pdf Tan, Bee Koon and Foo, Hooi Ling and Loh, Teck Chwen and Abdullah, Norhani and Idrus, Zulkifli (2005) Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography. Asian-Australasian Journal of Animal Sciences, 18 (12). pp. 1780-1785. ISSN 1011-2367; ESSN: 1976-5517 http://www.ajas.info/journal/view.php?number=21191 10.5713/ajas.2005.1780 |
| spellingShingle | Tan, Bee Koon Foo, Hooi Ling Loh, Teck Chwen Abdullah, Norhani Idrus, Zulkifli Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title | Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title_full | Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title_fullStr | Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title_full_unstemmed | Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title_short | Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| title_sort | purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography |
| url | http://psasir.upm.edu.my/id/eprint/49976/ http://psasir.upm.edu.my/id/eprint/49976/ http://psasir.upm.edu.my/id/eprint/49976/ http://psasir.upm.edu.my/id/eprint/49976/1/Purification%20and%20characterization%20of%20very%20low%20density%20lipoprotein%20in%20commercial%20broiler%20and%20crossbred%20village%20chickens%20by%20fast%20protein%20liquid%20chromatography.pdf |