Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study

This study was to investigate the occurrence of cross-contamination and decontamination in the kitchen via Listeria monocytogenes contaminated vegetables during salad preparation. In this study, naturally contaminated produce were used to provide realistic quantitative data as opposed to information...

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Main Authors: Ponniah, Jeyaletchumi, Robin, Tunung, Paie, Margaret Selina, Chai, Lay Ching, Radu, Son, Mohamad Ghazali, Farinazleen, Cheah, Yoke Kqueen, Nishibuchi, Mitsuaki, Nakaguchi, Yoshitsugu, Malakar, Pradeep Kumar
Format: Article
Language:English
Published: Malaysian Agricultural Research and Development Institute (MARDI) 2012
Online Access:http://psasir.upm.edu.my/id/eprint/49302/
http://psasir.upm.edu.my/id/eprint/49302/1/Assessment%20of%20Listeria%20monocytogenes%20in%20salad%20vegetables%20through%20kitchen%20stimulation%20study.pdf
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author Ponniah, Jeyaletchumi
Robin, Tunung
Paie, Margaret Selina
Chai, Lay Ching
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Malakar, Pradeep Kumar
author_facet Ponniah, Jeyaletchumi
Robin, Tunung
Paie, Margaret Selina
Chai, Lay Ching
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Malakar, Pradeep Kumar
author_sort Ponniah, Jeyaletchumi
building UPM Institutional Repository
collection Online Access
description This study was to investigate the occurrence of cross-contamination and decontamination in the kitchen via Listeria monocytogenes contaminated vegetables during salad preparation. In this study, naturally contaminated produce were used to provide realistic quantitative data as opposed to information obtained through artificial inoculation. The study was designed to simulate the real preparation of salad in kitchens in Malaysia which simply involved washing the vegetables in tap water and cutting them on a chopping board prior to serving. It was found that the mean percentage of transfer rates for L. monocytogenes from vegetables to wash water was 32.4–60.2%; from wash water to cucumber 24.9–66.3%; from vegetables to chopping board 18.9–32.2%; from chopping board to cucumber 5.4–75.3%. Washing of the vegetables in tap water caused a 0.3-log reduction of L. monocytogenes attached to the vegetables.
first_indexed 2025-11-15T10:18:55Z
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T10:18:55Z
publishDate 2012
publisher Malaysian Agricultural Research and Development Institute (MARDI)
recordtype eprints
repository_type Digital Repository
spelling upm-493022016-12-30T02:30:56Z http://psasir.upm.edu.my/id/eprint/49302/ Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study Ponniah, Jeyaletchumi Robin, Tunung Paie, Margaret Selina Chai, Lay Ching Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar This study was to investigate the occurrence of cross-contamination and decontamination in the kitchen via Listeria monocytogenes contaminated vegetables during salad preparation. In this study, naturally contaminated produce were used to provide realistic quantitative data as opposed to information obtained through artificial inoculation. The study was designed to simulate the real preparation of salad in kitchens in Malaysia which simply involved washing the vegetables in tap water and cutting them on a chopping board prior to serving. It was found that the mean percentage of transfer rates for L. monocytogenes from vegetables to wash water was 32.4–60.2%; from wash water to cucumber 24.9–66.3%; from vegetables to chopping board 18.9–32.2%; from chopping board to cucumber 5.4–75.3%. Washing of the vegetables in tap water caused a 0.3-log reduction of L. monocytogenes attached to the vegetables. Malaysian Agricultural Research and Development Institute (MARDI) 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/49302/1/Assessment%20of%20Listeria%20monocytogenes%20in%20salad%20vegetables%20through%20kitchen%20stimulation%20study.pdf Ponniah, Jeyaletchumi and Robin, Tunung and Paie, Margaret Selina and Chai, Lay Ching and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Malakar, Pradeep Kumar (2012) Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study. Journal of Tropical Agriculture and Food Science, 40 (1). pp. 55-62. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/64-2012/volume-40-no1/246-400106
spellingShingle Ponniah, Jeyaletchumi
Robin, Tunung
Paie, Margaret Selina
Chai, Lay Ching
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Malakar, Pradeep Kumar
Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title_full Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title_fullStr Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title_full_unstemmed Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title_short Assessment of Listeria monocytogenes in salad vegetables through kitchen stimulation study
title_sort assessment of listeria monocytogenes in salad vegetables through kitchen stimulation study
url http://psasir.upm.edu.my/id/eprint/49302/
http://psasir.upm.edu.my/id/eprint/49302/
http://psasir.upm.edu.my/id/eprint/49302/1/Assessment%20of%20Listeria%20monocytogenes%20in%20salad%20vegetables%20through%20kitchen%20stimulation%20study.pdf