Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus
Specific activity of lipase extracted from the mycelium of Aspergillus flavus, obtained from 3-day-old culture, increased nearly two times higher than from its culture supernatant. Different types of parameters were tested to optimise the extraction of mycelium-bound lipase. Highest specific activit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48935/ http://psasir.upm.edu.my/id/eprint/48935/1/Improvement%20of%20the%20specific%20activity%20of%20the%20extracted%20mycelium-bound%20lipase%20of%20Aspergillus%20flavus.pdf |
| _version_ | 1848851225230966784 |
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| author | Mohd Danial, Azlina Long, Kamariah Mohd Ghazali, Hasanah |
| author_facet | Mohd Danial, Azlina Long, Kamariah Mohd Ghazali, Hasanah |
| author_sort | Mohd Danial, Azlina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Specific activity of lipase extracted from the mycelium of Aspergillus flavus, obtained from 3-day-old culture, increased nearly two times higher than from its culture supernatant. Different types of parameters were tested to optimise the extraction of mycelium-bound lipase. Highest specific activity of A. flavus lipase was obtained at 30 °C for 30 min with the agitation rate of 200 rev/min. Tris-HCl buffer (0.05 M) at pH 8.2 was the best extractant followed by Triton X-100 (0.1%), EDTA (0.01 M), NaCl (1 M) and Tween-80 (0.01%). The optimised conditions developed for the extraction of mycelium-bound lipase improved the purity of this lipase by 2.9 times higher than the control. |
| first_indexed | 2025-11-15T10:18:48Z |
| format | Article |
| id | upm-48935 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:18:48Z |
| publishDate | 2004 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-489352016-12-29T09:58:46Z http://psasir.upm.edu.my/id/eprint/48935/ Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus Mohd Danial, Azlina Long, Kamariah Mohd Ghazali, Hasanah Specific activity of lipase extracted from the mycelium of Aspergillus flavus, obtained from 3-day-old culture, increased nearly two times higher than from its culture supernatant. Different types of parameters were tested to optimise the extraction of mycelium-bound lipase. Highest specific activity of A. flavus lipase was obtained at 30 °C for 30 min with the agitation rate of 200 rev/min. Tris-HCl buffer (0.05 M) at pH 8.2 was the best extractant followed by Triton X-100 (0.1%), EDTA (0.01 M), NaCl (1 M) and Tween-80 (0.01%). The optimised conditions developed for the extraction of mycelium-bound lipase improved the purity of this lipase by 2.9 times higher than the control. Malaysian Agricultural Research and Development Institute (MARDI) 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48935/1/Improvement%20of%20the%20specific%20activity%20of%20the%20extracted%20mycelium-bound%20lipase%20of%20Aspergillus%20flavus.pdf Mohd Danial, Azlina and Long, Kamariah and Mohd Ghazali, Hasanah (2004) Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus. Journal of Tropical Agriculture and Food Science, 32 (2). pp. 187-195. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/75-2004/volume-32-no2/705-320213 |
| spellingShingle | Mohd Danial, Azlina Long, Kamariah Mohd Ghazali, Hasanah Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title | Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title_full | Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title_fullStr | Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title_full_unstemmed | Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title_short | Improvement of the specific activity of the extracted mycelium-bound lipase of Aspergillus flavus |
| title_sort | improvement of the specific activity of the extracted mycelium-bound lipase of aspergillus flavus |
| url | http://psasir.upm.edu.my/id/eprint/48935/ http://psasir.upm.edu.my/id/eprint/48935/ http://psasir.upm.edu.my/id/eprint/48935/1/Improvement%20of%20the%20specific%20activity%20of%20the%20extracted%20mycelium-bound%20lipase%20of%20Aspergillus%20flavus.pdf |