Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus

Treatment of Citrus hystrix callus with various types of elicitors decreased the callus growth as the concentration of elicitors increased. However, callus growth remained relatively constant at 0.05% (w/v) alginate and slightly increased to 1.30 ± 0.04 g dwt./culture at 0.1% (w/v) alginate. Analysi...

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Main Authors: Roowi, Suri, Muse, Radzali, Mahmood, Marziah, Sukari, Mohd Aspollah
Format: Article
Language:English
Published: Malaysian Agricultural Research and Development Institute (MARDI) 2002
Online Access:http://psasir.upm.edu.my/id/eprint/48930/
http://psasir.upm.edu.my/id/eprint/48930/1/Effect%20of%20elicitors%20on%20the%20production%20of%20naringin%20and%20rutin%20in%20leech%20lime%20%28Citrus%20hystrix%29%20callus.pdf
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author Roowi, Suri
Muse, Radzali
Mahmood, Marziah
Sukari, Mohd Aspollah
author_facet Roowi, Suri
Muse, Radzali
Mahmood, Marziah
Sukari, Mohd Aspollah
author_sort Roowi, Suri
building UPM Institutional Repository
collection Online Access
description Treatment of Citrus hystrix callus with various types of elicitors decreased the callus growth as the concentration of elicitors increased. However, callus growth remained relatively constant at 0.05% (w/v) alginate and slightly increased to 1.30 ± 0.04 g dwt./culture at 0.1% (w/v) alginate. Analysis of the flavonoids using high performance liquid chromatography (HPLC) showed that only naringin and rutin were produced. Maximum production of naringin (12.13 ± 0.07 mg/g dwt.) and rutin (3.09 ± 0.05 mg/g dwt.) (p ≤ 0.05) was found in callus treated with 0.5% (w/v) agarose.
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spelling upm-489302016-12-29T09:58:40Z http://psasir.upm.edu.my/id/eprint/48930/ Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus Roowi, Suri Muse, Radzali Mahmood, Marziah Sukari, Mohd Aspollah Treatment of Citrus hystrix callus with various types of elicitors decreased the callus growth as the concentration of elicitors increased. However, callus growth remained relatively constant at 0.05% (w/v) alginate and slightly increased to 1.30 ± 0.04 g dwt./culture at 0.1% (w/v) alginate. Analysis of the flavonoids using high performance liquid chromatography (HPLC) showed that only naringin and rutin were produced. Maximum production of naringin (12.13 ± 0.07 mg/g dwt.) and rutin (3.09 ± 0.05 mg/g dwt.) (p ≤ 0.05) was found in callus treated with 0.5% (w/v) agarose. Malaysian Agricultural Research and Development Institute (MARDI) 2002 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48930/1/Effect%20of%20elicitors%20on%20the%20production%20of%20naringin%20and%20rutin%20in%20leech%20lime%20%28Citrus%20hystrix%29%20callus.pdf Roowi, Suri and Muse, Radzali and Mahmood, Marziah and Sukari, Mohd Aspollah (2002) Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus. Journal of Tropical Agriculture and Food Science, 30 (1). pp. 89-94. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/80-2002/volume-30-no1/302-300102
spellingShingle Roowi, Suri
Muse, Radzali
Mahmood, Marziah
Sukari, Mohd Aspollah
Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title_full Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title_fullStr Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title_full_unstemmed Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title_short Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus
title_sort effect of elicitors on the production of naringin and rutin in leech lime (citrus hystrix) callus
url http://psasir.upm.edu.my/id/eprint/48930/
http://psasir.upm.edu.my/id/eprint/48930/
http://psasir.upm.edu.my/id/eprint/48930/1/Effect%20of%20elicitors%20on%20the%20production%20of%20naringin%20and%20rutin%20in%20leech%20lime%20%28Citrus%20hystrix%29%20callus.pdf