Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test
Of the 148 cockle (Anadara granosa) and 433 shrimp (Paneus indicus) samples examined, 27% and 6.9% were positive for Vibrio vulnificus, respectively. Twenty-nine and 21 isolates from cockles and shrimps were examined for their antibiotic resistance. All isolates showed resistance to one or more of t...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48927/ http://psasir.upm.edu.my/id/eprint/48927/1/Characterization%20of%20Vibrio%20vulnificus%20isolated%20from%20retail%20cockle%20and%20shrimp%20by%20plasmid%20profiling%20and%20antibiotic%20susceptibility%20test.pdf |
| _version_ | 1848851223055171584 |
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| author | Radu, Son Tengku Ahmad, Tengku Ahbrizal Farizal Abd Mutalib, Sahilah |
| author_facet | Radu, Son Tengku Ahmad, Tengku Ahbrizal Farizal Abd Mutalib, Sahilah |
| author_sort | Radu, Son |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Of the 148 cockle (Anadara granosa) and 433 shrimp (Paneus indicus) samples examined, 27% and 6.9% were positive for Vibrio vulnificus, respectively. Twenty-nine and 21 isolates from cockles and shrimps were examined for their antibiotic resistance. All isolates showed resistance to one or more of the antibiotics tested. In transconjugation tests, no relationship was found between the high molecular weight plasmid (35.8 MDa) detected in several isolates and their resistance phenotypes, indicating that their antibiotic resistance is chromosomal. Plasmid profiles and antibiotic resistance patterns were used as a preliminary approach to type, at strain level, the isolates from cockles and shrimps. Analysis by antibiotic resistance patterns showed a high phenotypic polymorphism. However, the high number of isolates devoid of plasmid rendered this technique less useful. These results indicate that multiresistant V. vulnificus isolates exhibiting genotypic or phenotypic variations are easily recovered from cockles and shrimps in the study area, posing a potential public health risk. |
| first_indexed | 2025-11-15T10:18:46Z |
| format | Article |
| id | upm-48927 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:18:46Z |
| publishDate | 2002 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-489272016-12-29T09:58:35Z http://psasir.upm.edu.my/id/eprint/48927/ Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test Radu, Son Tengku Ahmad, Tengku Ahbrizal Farizal Abd Mutalib, Sahilah Of the 148 cockle (Anadara granosa) and 433 shrimp (Paneus indicus) samples examined, 27% and 6.9% were positive for Vibrio vulnificus, respectively. Twenty-nine and 21 isolates from cockles and shrimps were examined for their antibiotic resistance. All isolates showed resistance to one or more of the antibiotics tested. In transconjugation tests, no relationship was found between the high molecular weight plasmid (35.8 MDa) detected in several isolates and their resistance phenotypes, indicating that their antibiotic resistance is chromosomal. Plasmid profiles and antibiotic resistance patterns were used as a preliminary approach to type, at strain level, the isolates from cockles and shrimps. Analysis by antibiotic resistance patterns showed a high phenotypic polymorphism. However, the high number of isolates devoid of plasmid rendered this technique less useful. These results indicate that multiresistant V. vulnificus isolates exhibiting genotypic or phenotypic variations are easily recovered from cockles and shrimps in the study area, posing a potential public health risk. Malaysian Agricultural Research and Development Institute (MARDI) 2002 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48927/1/Characterization%20of%20Vibrio%20vulnificus%20isolated%20from%20retail%20cockle%20and%20shrimp%20by%20plasmid%20profiling%20and%20antibiotic%20susceptibility%20test.pdf Radu, Son and Tengku Ahmad, Tengku Ahbrizal Farizal and Abd Mutalib, Sahilah (2002) Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test. Journal of Tropical Agriculture and Food Science, 30 (1). pp. 31-37. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/80-2002/volume-30-no1/348-300113 |
| spellingShingle | Radu, Son Tengku Ahmad, Tengku Ahbrizal Farizal Abd Mutalib, Sahilah Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title | Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title_full | Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title_fullStr | Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title_full_unstemmed | Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title_short | Characterization of Vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| title_sort | characterization of vibrio vulnificus isolated from retail cockle and shrimp by plasmid profiling and antibiotic susceptibility test |
| url | http://psasir.upm.edu.my/id/eprint/48927/ http://psasir.upm.edu.my/id/eprint/48927/ http://psasir.upm.edu.my/id/eprint/48927/1/Characterization%20of%20Vibrio%20vulnificus%20isolated%20from%20retail%20cockle%20and%20shrimp%20by%20plasmid%20profiling%20and%20antibiotic%20susceptibility%20test.pdf |