Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen
The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol. Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognize...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Japan Society for Bioscience, Biotechnology, and Agrochemistry
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48669/ http://psasir.upm.edu.my/id/eprint/48669/1/Structural%20features%20of%20N-glycans%20linked%20to%20glycoproteins%20from%20oil%20palm%20pollen%2C%20an%20allergenic%20pollen.pdf http://psasir.upm.edu.my/id/eprint/48669/7/bbb2232.pdf |
| _version_ | 1848851151900901376 |
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| author | Kimura, Yoshinobu Yoshiie, Takeo Kit, Woo Kwan Maeda, Megumi Kimura, Mariko Tan, Siang Hee |
| author_facet | Kimura, Yoshinobu Yoshiie, Takeo Kit, Woo Kwan Maeda, Megumi Kimura, Mariko Tan, Siang Hee |
| author_sort | Kimura, Yoshinobu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol. Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. In this report, we describe the structural analysis of sugar chains linked to palm pollen glycoproteins to confirm the ubiquitous occurrence of antigenic N-glycans in the allergenic pollen. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine followed by purification with a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, electrospray ionization mass spectrometry (ESI-MS), and tandem MS analysis, as well as exoglycosidase digestions. The antigenic N-glycan bearing α1-3 fucose and/or β1-2 xylose residues accounts for 36.9% of total N-glycans: GlcNAc2Man3Xyl1Fuc1GlcNAc2 (24.6%), GlcNAc2Man3Xyl1GlcNAc2 (4.4%), Man3Xyl1Fuc1-GlcNAc2 (1.1%), GlcNAc1Man3Xyl1Fuc1GlcNAc2 (5.6%), and GlcNAc1Man3Xyl1GlcNAc2 (1.2%). The remaining 63.1% of the total N-glycans belong to the high-mannose type structure: Man9GlcNAc2 (5.8%), Man8GlcNAc2 (32.1%), Man7GlcNAc2 (19.9%), Man6GlcNAc2 (5.3%). |
| first_indexed | 2025-11-15T10:17:38Z |
| format | Article |
| id | upm-48669 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T10:17:38Z |
| publishDate | 2003 |
| publisher | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-486692024-08-08T00:35:14Z http://psasir.upm.edu.my/id/eprint/48669/ Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen Kimura, Yoshinobu Yoshiie, Takeo Kit, Woo Kwan Maeda, Megumi Kimura, Mariko Tan, Siang Hee The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol. Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. In this report, we describe the structural analysis of sugar chains linked to palm pollen glycoproteins to confirm the ubiquitous occurrence of antigenic N-glycans in the allergenic pollen. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine followed by purification with a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, electrospray ionization mass spectrometry (ESI-MS), and tandem MS analysis, as well as exoglycosidase digestions. The antigenic N-glycan bearing α1-3 fucose and/or β1-2 xylose residues accounts for 36.9% of total N-glycans: GlcNAc2Man3Xyl1Fuc1GlcNAc2 (24.6%), GlcNAc2Man3Xyl1GlcNAc2 (4.4%), Man3Xyl1Fuc1-GlcNAc2 (1.1%), GlcNAc1Man3Xyl1Fuc1GlcNAc2 (5.6%), and GlcNAc1Man3Xyl1GlcNAc2 (1.2%). The remaining 63.1% of the total N-glycans belong to the high-mannose type structure: Man9GlcNAc2 (5.8%), Man8GlcNAc2 (32.1%), Man7GlcNAc2 (19.9%), Man6GlcNAc2 (5.3%). Japan Society for Bioscience, Biotechnology, and Agrochemistry 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/48669/1/Structural%20features%20of%20N-glycans%20linked%20to%20glycoproteins%20from%20oil%20palm%20pollen%2C%20an%20allergenic%20pollen.pdf text en http://psasir.upm.edu.my/id/eprint/48669/7/bbb2232.pdf Kimura, Yoshinobu and Yoshiie, Takeo and Kit, Woo Kwan and Maeda, Megumi and Kimura, Mariko and Tan, Siang Hee (2003) Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen. Bioscience, Biotechnology, and Biochemistry, 67 (10). pp. 2232-2239. ISSN 0916-8451; ESSN: 1347-6947 https://www.jstage.jst.go.jp/article/bbb/67/10/67_10_2232/_article 10.1271/bbb.67.2232 |
| spellingShingle | Kimura, Yoshinobu Yoshiie, Takeo Kit, Woo Kwan Maeda, Megumi Kimura, Mariko Tan, Siang Hee Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title | Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title_full | Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title_fullStr | Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title_full_unstemmed | Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title_short | Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| title_sort | structural features of n-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen |
| url | http://psasir.upm.edu.my/id/eprint/48669/ http://psasir.upm.edu.my/id/eprint/48669/ http://psasir.upm.edu.my/id/eprint/48669/ http://psasir.upm.edu.my/id/eprint/48669/1/Structural%20features%20of%20N-glycans%20linked%20to%20glycoproteins%20from%20oil%20palm%20pollen%2C%20an%20allergenic%20pollen.pdf http://psasir.upm.edu.my/id/eprint/48669/7/bbb2232.pdf |