Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines
The cytotoxic and genotoxic effects of goniothalamin, a plant styryllactone, were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the Alkaline Comet assay respectively in human leukemic cell lines. Following 72 h of treatment, the IC50 values of g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Japanese Environmental Mutagen Society
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48591/ http://psasir.upm.edu.my/id/eprint/48591/1/Evaluation%20of%20the%20cytotoxic%20and%20genotoxic%20effects%20of%20goniothalamin%20in%20leukemic%20cell%20lines.pdf |
| _version_ | 1848851129164627968 |
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| author | Rajab, Nor Fadilah Abd. Hamid, Zariyantey Hassan, Hunaizah Ali, Abdul Manaf Din, Laily Inayat Hussain, Salmaan Hussain |
| author_facet | Rajab, Nor Fadilah Abd. Hamid, Zariyantey Hassan, Hunaizah Ali, Abdul Manaf Din, Laily Inayat Hussain, Salmaan Hussain |
| author_sort | Rajab, Nor Fadilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The cytotoxic and genotoxic effects of goniothalamin, a plant styryllactone, were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the Alkaline Comet assay respectively in human leukemic cell lines. Following 72 h of treatment, the IC50 values of goniothalamin in human HL-60 promyelocytic leukemia cells and CEM-SS T-lymphoblastic cells were 4.5 μg/mL and 2.4 μg/mL respectively. The genotoxicity of goniothalamin in both HL-60 and CEM-SS cells was detected as early as 2 h following treatment at IC10 and IC25 concentrations. However, pretreatment with the antioxidant N-acetyl-cysteine (NAC) at 1 mM for 30 minutes did not abrogate genotoxicity of this compound. This result suggests that primary induction of DNA damage by goniothalamin may not involve oxidative damage. In conclusion, our results demonstrate genotoxic damage induced by goniothalamin in leukemic cells. Further studies are needed to ascertain the mode of action of goniothalamin in inducing DNA damage. |
| first_indexed | 2025-11-15T10:17:16Z |
| format | Article |
| id | upm-48591 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:17:16Z |
| publishDate | 2005 |
| publisher | The Japanese Environmental Mutagen Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-485912016-10-10T03:47:13Z http://psasir.upm.edu.my/id/eprint/48591/ Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines Rajab, Nor Fadilah Abd. Hamid, Zariyantey Hassan, Hunaizah Ali, Abdul Manaf Din, Laily Inayat Hussain, Salmaan Hussain The cytotoxic and genotoxic effects of goniothalamin, a plant styryllactone, were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the Alkaline Comet assay respectively in human leukemic cell lines. Following 72 h of treatment, the IC50 values of goniothalamin in human HL-60 promyelocytic leukemia cells and CEM-SS T-lymphoblastic cells were 4.5 μg/mL and 2.4 μg/mL respectively. The genotoxicity of goniothalamin in both HL-60 and CEM-SS cells was detected as early as 2 h following treatment at IC10 and IC25 concentrations. However, pretreatment with the antioxidant N-acetyl-cysteine (NAC) at 1 mM for 30 minutes did not abrogate genotoxicity of this compound. This result suggests that primary induction of DNA damage by goniothalamin may not involve oxidative damage. In conclusion, our results demonstrate genotoxic damage induced by goniothalamin in leukemic cells. Further studies are needed to ascertain the mode of action of goniothalamin in inducing DNA damage. The Japanese Environmental Mutagen Society 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48591/1/Evaluation%20of%20the%20cytotoxic%20and%20genotoxic%20effects%20of%20goniothalamin%20in%20leukemic%20cell%20lines.pdf Rajab, Nor Fadilah and Abd. Hamid, Zariyantey and Hassan, Hunaizah and Ali, Abdul Manaf and Din, Laily and Inayat Hussain, Salmaan Hussain (2005) Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines. Environmental Mutagen Research, 27 (3). pp. 161-164. ISSN 0910-0865; ESSN: 1880-7054 https://www.jstage.jst.go.jp/article/jems/27/3/27_3_161/_article 10.3123/jems.27.161 |
| spellingShingle | Rajab, Nor Fadilah Abd. Hamid, Zariyantey Hassan, Hunaizah Ali, Abdul Manaf Din, Laily Inayat Hussain, Salmaan Hussain Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title | Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title_full | Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title_fullStr | Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title_full_unstemmed | Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title_short | Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| title_sort | evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines |
| url | http://psasir.upm.edu.my/id/eprint/48591/ http://psasir.upm.edu.my/id/eprint/48591/ http://psasir.upm.edu.my/id/eprint/48591/ http://psasir.upm.edu.my/id/eprint/48591/1/Evaluation%20of%20the%20cytotoxic%20and%20genotoxic%20effects%20of%20goniothalamin%20in%20leukemic%20cell%20lines.pdf |