High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

QR Code

High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

Monoclonal antibody (mAb) is a specific protein molecule that is widely used in biopharmaceutical industry for therapeutic and diagnostic application. The purification of the mAb products typically involved Protein-A affinity chromatography as the first capture step driven by the very specific bindi...

Full description

Bibliographic Details
Main Author:	Pakiman, Norazrina
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Proteins - Purification Cell culture Chromatographic analysis
Online Access:	http://psasir.upm.edu.my/id/eprint/48480/ http://psasir.upm.edu.my/id/eprint/48480/1/FK%202012%20123R.pdf

Similar Items

Purification of histidine-tagged nucleocapsid protein of Nipah virus using immobilized metal affinity chromatography
by: Fui , Chin Chong, et al.
Published: (2009)

The Regulation of Peptide Mimotope/Epitope Recognition by Monoclonal Antibodies
by: Smith, Richard Gary
Published: (2002)

Chromatic Equivalence Classes and Chromatic Defining Numbers of Certain Graphs
by: Omoomi, Behnaz
Published: (2001)

Determination of amoxicillin cross-contamination in Ibuprofen tablets using ultra performance liquid chromatography and IDEXX snap kit
by: Ali, Mohamed Ali Mohamed
Published: (2017)

Purification on a Recombinant Nucleocapsid Protein of Newcastle Disease Virus Using Expanded Bed Adsorption Chromatography
by: Tan, Yan Peng
Published: (2005)

Recovery and purification of epidermal growth factor from fermentation broth using expanded bed chromatography
by: Rosti, Ira Amira
Published: (2013)

Development and validation of amino acid analysis methods in gelatin and gelatin-based products using high-performance liquid chromatography
by: Mohd Ismail, Azilawati
Published: (2016)

Mapping Of Nucleocapsid Protein (Np) Epitopes and Np-Phosphoprotein Interactive Domains of Newcastle Disease Virus with Np Monoclonal Antibodies
by: Ahmad Raus, Raha
Published: (2006)

Chemical Constituents and Biological Activity of Kaempferia Angustifolia, K. Rotunda,Spermacoce Articularis and S. Exilis
by: Neoh, Bee Keat
Published: (2006)

Detection and quantification of alcohol compounds in food and beverages using static headspace-gas chromatography/mass spectrometry
by: Law, Se Vern
Published: (2012)

Overexpression and crystallization of 205Y lipase from Bacillus sphaericus 205Y
by: Hamooni, Sayyedeh Hoda
Published: (2017)

Purification of long helical capsid of newcastle disease virus from Escherichia coli
by: Yap, Chee Fai
Published: (2013)

Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system
by: Dezfooli, Seyedehsara Masoomi, et al.
Published: (2016)

Development of dye affinity adsorbents for recovery of polyclonal anti-hepatitis B core antigen immunoglobulin G
by: Wongchuphan, Rattana
Published: (2010)

Chromatographic Separation of Preconcentrated Vitamin E from Palm Fatty Acid Distillate
by: Chu, Boon Seang
Published: (2004)

Optimization of serum free medium for the production of humanized anti-cea monoclonal antibody.
by: Ab Latif, Nurzila
Published: (2011)

Expression, purification and crystallisation of DNAse and immunity proteins in the S3 pyocin of Pseudomonas aeruginosa
by: Newman, Christopher James
Published: (2017)

Cell-free protein synthesis for on-site and on-demand biomanufacturing in extreme and low resource environments
by: Shivakumar, Tejasvi
Published: (2024)

A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo
by: Desombere, Isabelle, et al.
Published: (2017)

Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1
by: Mesalam, Ahmed Atef, et al.
Published: (2018)

The performance of a glass bead shaking technique for the disruption of Escherichia coli cells
by: Ramakrishnan, Nagasundara Ramanan, et al.
Published: (2008)

Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus
by: Ahmad Raus, Raha, et al.
Published: (2007)

Penggunaan Kaedah 2d-Page Untuk Kajian Pengekspresan Protein Semasa Embriogenesis Puntius gonionotus
by: Ahmat @ Amiruddin, Suhaida
Published: (2004)

Two methods for three - dimensional culture: addition of exogenous ECM components and capture of endogenous ECM components
by: McGinlay, Sarah
Published: (2023)

Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells
by: Tey, Beng Ti, et al.
Published: (2005)

Molecular expression of recombinant Apoptin in planta and preliminary evaluation of biological characteristics of plant-made Apoptin on cancerous cells
by: Chan, Xiao Ying
Published: (2017)

Characterisation of anti-glycan monoclonal antibodies
by: Noble, Philip W.
Published: (2011)

Optimization and enzymatic hydrolysis of Tilapia by-product and fractionation of protein hydrolysate using membrane ultrafiltration
by: Roslan, Jumardi
Published: (2016)

MALDI-target integrated platform for affinity-captured protein digestion
by: Ahmad Tajudin, Asilah, et al.
Published: (2014)

Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies
by: Ahmad-Raus, Raha, et al.
Published: (2009)

Generation Of A Naïve Human Scfv Antibody Library For The Production Of Human Monoclonal Antibodies By Using Phage Display Technology
by: Lim , Bee Nar
Published: (2015)

The challenges of dna extraction in different assorted food matrices: a review
by: Wong, Sie Chuong, et al.
Published: (2018)

The generation and characterisation of anti-glycan monoclonal antibodies
by: Chua, Jia Xin
Published: (2017)

Predicting response to monoclonal antibody in severe asthma
by: Pang, Yik Lam
Published: (2022)

Generation and validation of Aspergillus fumigatus monoclonal antibodies
by: Jafarlou, Mahdi
Published: (2009)

Reversible, High-Affinity Surface Capturing of Proteins Directed by Supramolecular Assembly
by: Di Palma, Giuseppe, et al.
Published: (2019)

Regulation of human heterochromatin protein 1 binding protein 3 (HP1BP3, also known as HP1BP74) in response to the DNA damage
by: Normatova, Makhliyo
Published: (2016)

Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter
by: Yong, Wilson Thau Lim, et al.
Published: (2006)

Development Of A Naïve Human Fab Antibody Library To Generate Monoclonal Antibodies Against Bmsxp Recombinant Antigen
by: Omar, Noorsharmimi
Published: (2015)

Impact of p38 mitogen-activated protein kinase inhibition on immunostimulatory properties of human 6-sulfo LacNAc dendritic cells
by: Langosch, Saskia, et al.
Published: (2016)