Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake
Palm kernel cake (PKC), a by-product of the palm kernel oil extraction process contains mannan as its main polysaccharide. Mixed culture microbial degradation may enhance mannan-degrading enzymes production. Therefore, the objective of the study was to examine the nature of mycelial interactions and...
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/4790/ http://psasir.upm.edu.my/id/eprint/4790/1/Mycelial_growth_interactions.pdf |
| _version_ | 1848839916629262336 |
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| author | Buchi, Iluyemi Florence Musa, Mohamed Hanafi |
| author_facet | Buchi, Iluyemi Florence Musa, Mohamed Hanafi |
| author_sort | Buchi, Iluyemi Florence |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Palm kernel cake (PKC), a by-product of the palm kernel oil extraction process contains mannan as its main polysaccharide. Mixed culture microbial degradation may enhance mannan-degrading enzymes production. Therefore, the objective of the study was to examine the nature of mycelial interactions and corresponding production of mannan-degrading enzymes of PKC. Fungal interactions was carried out using Sclerotium rolfsii and Aspergillus niger co-cultured with three Trichoderma strains (Trichoderma harzianum, Trichoderma longiobrachiatum and Trichoderma koningii) on potato dextrose agar (PDA) in disposable petri-dishes. Measurements of growth diameters were taken on days 2 and 13. For mannan-degrading enzyme production, single and co-cultures of these fungi were carried out under submerged cultivation for 13 days with PKC as the carbon source. About 57% of observed interactions on PDA were deadlock, 29% replacement and 14% intermingling. In Trichoderma sp./A. niger mixed cultures, there was an overall significant enhancement of enzyme: 2 to 200 fold (β-D-mannanase), 8 to 25 fold (β-mannosidase) and from no change to 15 fold increase (α-galactosidase). There was no obvious relationship between enzyme production and protein yield. However, co-culturing of A. niger with the Trichoderma strains showed an enhancement of mannan-degrading enzyme activities without reducing biomass yield. |
| first_indexed | 2025-11-15T07:19:03Z |
| format | Article |
| id | upm-4790 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:19:03Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| recordtype | eprints |
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| spelling | upm-47902017-11-09T08:17:26Z http://psasir.upm.edu.my/id/eprint/4790/ Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake Buchi, Iluyemi Florence Musa, Mohamed Hanafi Palm kernel cake (PKC), a by-product of the palm kernel oil extraction process contains mannan as its main polysaccharide. Mixed culture microbial degradation may enhance mannan-degrading enzymes production. Therefore, the objective of the study was to examine the nature of mycelial interactions and corresponding production of mannan-degrading enzymes of PKC. Fungal interactions was carried out using Sclerotium rolfsii and Aspergillus niger co-cultured with three Trichoderma strains (Trichoderma harzianum, Trichoderma longiobrachiatum and Trichoderma koningii) on potato dextrose agar (PDA) in disposable petri-dishes. Measurements of growth diameters were taken on days 2 and 13. For mannan-degrading enzyme production, single and co-cultures of these fungi were carried out under submerged cultivation for 13 days with PKC as the carbon source. About 57% of observed interactions on PDA were deadlock, 29% replacement and 14% intermingling. In Trichoderma sp./A. niger mixed cultures, there was an overall significant enhancement of enzyme: 2 to 200 fold (β-D-mannanase), 8 to 25 fold (β-mannosidase) and from no change to 15 fold increase (α-galactosidase). There was no obvious relationship between enzyme production and protein yield. However, co-culturing of A. niger with the Trichoderma strains showed an enhancement of mannan-degrading enzyme activities without reducing biomass yield. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4790/1/Mycelial_growth_interactions.pdf Buchi, Iluyemi Florence and Musa, Mohamed Hanafi (2009) Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake. African Journal of Biotechnology, 8 (10). art. no. 7B46A948018. pp. 2283-2288. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/7B46A948018 |
| spellingShingle | Buchi, Iluyemi Florence Musa, Mohamed Hanafi Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title | Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title_full | Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title_fullStr | Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title_full_unstemmed | Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title_short | Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| title_sort | mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake |
| url | http://psasir.upm.edu.my/id/eprint/4790/ http://psasir.upm.edu.my/id/eprint/4790/ http://psasir.upm.edu.my/id/eprint/4790/1/Mycelial_growth_interactions.pdf |