Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology
BACKGROUND: Adipate esters are used as low-temperature and low-viscosity plasticizers for polyvinyl chloride and its copolymers. In this work, optimization of lipase-catalyzed production of dilauryl adipate was carried out using response surface methodology (RSM) based on a four-factor-five-level...
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/4775/ http://psasir.upm.edu.my/id/eprint/4775/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20synthesis%20of%20dilauryl.pdf |
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| author | Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Basri, Mahiran Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Md Radzi, Salina |
| author_facet | Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Basri, Mahiran Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Md Radzi, Salina |
| author_sort | Abdul Rahman, Mohd Basyaruddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: Adipate esters are used as low-temperature and low-viscosity plasticizers for polyvinyl chloride
and its copolymers. In this work, optimization of lipase-catalyzed production of dilauryl adipate was carried out
using response surface methodology (RSM) based on a four-factor-five-level central composite rotatable design
(CCRD). Immobilized lipase from Candida antarctica (Novozym 435) was used as catalyst in this reaction. Various
reaction parameters affecting the synthesis of adipate ester, including alcohol/acidmolar ratio, amount of enzyme,
temperature and reaction time, were investigated.
RESULTS: Statistical analysis showed that the amount of enzyme was less significant than the other three factors.
The optimal conditions for the enzymatic reaction were obtained at 5.7:1 substrate molar ratio using 0.18 g of
enzyme at 53.1 ◦C for 282.2min. Under these conditions the esterification percentage was 96.0%.
CONCLUSIONS: The results demonstrated that response surface methodology can be applied effectively to
optimize the lipase-catalyzed synthesis of adipate ester. The optimum conditions can obtained be used to scale up
the process. |
| first_indexed | 2025-11-15T07:19:00Z |
| format | Article |
| id | upm-4775 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:19:00Z |
| publishDate | 2008 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-47752016-09-27T07:19:04Z http://psasir.upm.edu.my/id/eprint/4775/ Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Basri, Mahiran Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Md Radzi, Salina BACKGROUND: Adipate esters are used as low-temperature and low-viscosity plasticizers for polyvinyl chloride and its copolymers. In this work, optimization of lipase-catalyzed production of dilauryl adipate was carried out using response surface methodology (RSM) based on a four-factor-five-level central composite rotatable design (CCRD). Immobilized lipase from Candida antarctica (Novozym 435) was used as catalyst in this reaction. Various reaction parameters affecting the synthesis of adipate ester, including alcohol/acidmolar ratio, amount of enzyme, temperature and reaction time, were investigated. RESULTS: Statistical analysis showed that the amount of enzyme was less significant than the other three factors. The optimal conditions for the enzymatic reaction were obtained at 5.7:1 substrate molar ratio using 0.18 g of enzyme at 53.1 ◦C for 282.2min. Under these conditions the esterification percentage was 96.0%. CONCLUSIONS: The results demonstrated that response surface methodology can be applied effectively to optimize the lipase-catalyzed synthesis of adipate ester. The optimum conditions can obtained be used to scale up the process. Society of Chemical Industry 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4775/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20synthesis%20of%20dilauryl.pdf Abdul Rahman, Mohd Basyaruddin and Chaibakhsh, Naz and Basri, Mahiran and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Md Radzi, Salina (2008) Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology. Journal of Chemical Technology and Biotechnology, 83 (11). pp. 1534-1540. ISSN 0268-2575; ESSN: 1097-4660 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jctb.1960/abstract 10.1002/jctb.1960 |
| spellingShingle | Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Basri, Mahiran Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Md Radzi, Salina Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title | Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title_full | Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title_fullStr | Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title_full_unstemmed | Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title_short | Modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| title_sort | modeling and optimization of lipase-catalyzed synthesis of dilauryl adipate ester by response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/4775/ http://psasir.upm.edu.my/id/eprint/4775/ http://psasir.upm.edu.my/id/eprint/4775/ http://psasir.upm.edu.my/id/eprint/4775/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20synthesis%20of%20dilauryl.pdf |