Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli
EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/47431/ http://psasir.upm.edu.my/id/eprint/47431/1/Cloning%20and%20expression%20of%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20type%202%20ribosome%20inactivating%20protein%20in%20Escherichia%20coli.pdf |
| _version_ | 1848850814948343808 |
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| author | Tan, Yung Chie Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu Wong, Mui Yun Ho, Chai Ling |
| author_facet | Tan, Yung Chie Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu Wong, Mui Yun Ho, Chai Ling |
| author_sort | Tan, Yung Chie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields. |
| first_indexed | 2025-11-15T10:12:17Z |
| format | Article |
| id | upm-47431 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:12:17Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-474312016-05-20T01:17:33Z http://psasir.upm.edu.my/id/eprint/47431/ Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli Tan, Yung Chie Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu Wong, Mui Yun Ho, Chai Ling EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields. Springer 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47431/1/Cloning%20and%20expression%20of%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20type%202%20ribosome%20inactivating%20protein%20in%20Escherichia%20coli.pdf Tan, Yung Chie and Ho, Wan Yong and Mohammed Alitheen, Noorjahan Banu and Wong, Mui Yun and Ho, Chai Ling (2016) Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli. International Journal of Peptide Research and Therapeutics, 22 (1). pp. 37-44. ISSN 1573-3149; ESSN: 1573-3904 http://link.springer.com/article/10.1007%2Fs10989-015-9485-5 10.1007/s10989-015-9485-5 |
| spellingShingle | Tan, Yung Chie Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu Wong, Mui Yun Ho, Chai Ling Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title | Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title_full | Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title_fullStr | Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title_full_unstemmed | Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title_short | Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli |
| title_sort | cloning and expression of oil palm (elaeis guineensis jacq.) type 2 ribosome inactivating protein in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/47431/ http://psasir.upm.edu.my/id/eprint/47431/ http://psasir.upm.edu.my/id/eprint/47431/ http://psasir.upm.edu.my/id/eprint/47431/1/Cloning%20and%20expression%20of%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20type%202%20ribosome%20inactivating%20protein%20in%20Escherichia%20coli.pdf |