Cloning and expression of oil palm (Elaeis guineensis Jacq.) type 2 ribosome inactivating protein in Escherichia coli

EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant...

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Bibliographic Details
Main Authors: Tan, Yung Chie, Ho, Wan Yong, Mohammed Alitheen, Noorjahan Banu, Wong, Mui Yun, Ho, Chai Ling
Format: Article
Language:English
Published: Springer 2016
Online Access:http://psasir.upm.edu.my/id/eprint/47431/
http://psasir.upm.edu.my/id/eprint/47431/1/Cloning%20and%20expression%20of%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20type%202%20ribosome%20inactivating%20protein%20in%20Escherichia%20coli.pdf
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Summary:EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields.