Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces
Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temp...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/47399/ http://psasir.upm.edu.my/id/eprint/47399/1/Continuous%20harvest%20of%20stem%20cells%20via%20partial%20detachment%20from%20thermoresponsive%20nanobrush%20surfaces.pdf |
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| author | Peng, I. Chia Yeh, Chin Chen Lu, Yi Tung Muduli, Saradaprasan Ling, Qing Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Subbiah, Suresh Kumar Murugan, Kadarkarai Lee, Hsin Chung Chang, Yung Higuchi, Akon |
| author_facet | Peng, I. Chia Yeh, Chin Chen Lu, Yi Tung Muduli, Saradaprasan Ling, Qing Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Subbiah, Suresh Kumar Murugan, Kadarkarai Lee, Hsin Chung Chang, Yung Higuchi, Akon |
| author_sort | Peng, I. Chia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. The detached stem cells are harvested by exchange into fresh culture medium. Following this, the remaining cells are continuously cultured by expansion in fresh culture medium at 37 °C. Thermoresponsive nanobrush surfaces were prepared by coating block copolymers containing polystyrene (for hydrophobic anchoring onto culture dishes) with three types of polymers: (a) polyacrylic acid with cell-binding oligopeptides, (b) thermoresponsive poly-N-isopropylacrylamide, and (c) hydrophilic poly(ethyleneglycol)methacrylate. The optimal coating durations and compositions for these copolymers to facilitate adequate attachment and detachment of human adipose-derived stem cells (hADSCs) and embryonic stem cells (hESCs) were determined. hADSCs and hESCs were continuously harvested for 5 and 3 cycles, respectively, via the partial detachment of cells from thermoresponsive nanobrush surfaces. |
| first_indexed | 2025-11-15T10:12:09Z |
| format | Article |
| id | upm-47399 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:12:09Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-473992016-05-19T07:13:18Z http://psasir.upm.edu.my/id/eprint/47399/ Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces Peng, I. Chia Yeh, Chin Chen Lu, Yi Tung Muduli, Saradaprasan Ling, Qing Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Subbiah, Suresh Kumar Murugan, Kadarkarai Lee, Hsin Chung Chang, Yung Higuchi, Akon Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. The detached stem cells are harvested by exchange into fresh culture medium. Following this, the remaining cells are continuously cultured by expansion in fresh culture medium at 37 °C. Thermoresponsive nanobrush surfaces were prepared by coating block copolymers containing polystyrene (for hydrophobic anchoring onto culture dishes) with three types of polymers: (a) polyacrylic acid with cell-binding oligopeptides, (b) thermoresponsive poly-N-isopropylacrylamide, and (c) hydrophilic poly(ethyleneglycol)methacrylate. The optimal coating durations and compositions for these copolymers to facilitate adequate attachment and detachment of human adipose-derived stem cells (hADSCs) and embryonic stem cells (hESCs) were determined. hADSCs and hESCs were continuously harvested for 5 and 3 cycles, respectively, via the partial detachment of cells from thermoresponsive nanobrush surfaces. Elsevier 2016-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47399/1/Continuous%20harvest%20of%20stem%20cells%20via%20partial%20detachment%20from%20thermoresponsive%20nanobrush%20surfaces.pdf Peng, I. Chia and Yeh, Chin Chen and Lu, Yi Tung and Muduli, Saradaprasan and Ling, Qing Dong and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Subbiah, Suresh Kumar and Murugan, Kadarkarai and Lee, Hsin Chung and Chang, Yung and Higuchi, Akon (2016) Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces. Biomaterials, 76. pp. 76-86. ISSN 0142-9612; ESSN: 1878-5905 http://www.sciencedirect.com/science/article/pii/S0142961215008492 10.1016/j.biomaterials.2015.10.039 |
| spellingShingle | Peng, I. Chia Yeh, Chin Chen Lu, Yi Tung Muduli, Saradaprasan Ling, Qing Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Subbiah, Suresh Kumar Murugan, Kadarkarai Lee, Hsin Chung Chang, Yung Higuchi, Akon Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title | Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title_full | Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title_fullStr | Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title_full_unstemmed | Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title_short | Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| title_sort | continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces |
| url | http://psasir.upm.edu.my/id/eprint/47399/ http://psasir.upm.edu.my/id/eprint/47399/ http://psasir.upm.edu.my/id/eprint/47399/ http://psasir.upm.edu.my/id/eprint/47399/1/Continuous%20harvest%20of%20stem%20cells%20via%20partial%20detachment%20from%20thermoresponsive%20nanobrush%20surfaces.pdf |