Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)
Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/47379/ http://psasir.upm.edu.my/id/eprint/47379/1/Molecular%20detection%20and%20pathology%20of%20Pasteurella%20multocida%202%20in%20the%20reproductive%20system%20of%20pre-pubertal%20buffalo%20calves%20%28Bubalus%20bubalis%29.pdf |
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| author | Ibrahim, Hayder Hamzah Abba, Yusuf Ahmed, Ihsan Muneer Abdullah, Faez Firdaus Jesse Lim, Eric Teik Chung Marza, Ali Dhiaa Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abd Wahid Mohd Lila, Mohd Azmi |
| author_facet | Ibrahim, Hayder Hamzah Abba, Yusuf Ahmed, Ihsan Muneer Abdullah, Faez Firdaus Jesse Lim, Eric Teik Chung Marza, Ali Dhiaa Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abd Wahid Mohd Lila, Mohd Azmi |
| author_sort | Ibrahim, Hayder Hamzah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high mortality rates and indirect effects through impairment of the animal productivity. It is well known that HS affects mainly the respiratory and digestive tracts of host animals. However, involvement of the reproductive system in the pathogenesis of HS has not been reported previously. This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1 × 1012 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 h post-infection, while calves in group 2 were euthanized after day 21 post-infection. Successful isolation and PCR confirmation of P. multocida B:2 was achieved from different parts of the reproductive system, including ovary, oviduct, uterine horn, uterine body and vagina as well as mammary glands and supramammary lymph nodes of the buffaloes in group 1 after 12 h post-infection. However, P. multocida B:2 was not detected in the reproductive organs of buffaloes in group 2 and group 3 after 21 days post-infection. Significant differences (P < 0.05) were found in histopathological changes between the two groups, which mainly affected different anatomic regions of the reproductive system. This work provides an insight into the involvement of the female reproductive system of buffaloes during the pathogenesis of HS and shows that route of inoculation strongly affect the localization of the bacterium in the reproductive system. |
| first_indexed | 2025-11-15T10:12:05Z |
| format | Article |
| id | upm-47379 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:12:05Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-473792016-11-21T02:33:09Z http://psasir.upm.edu.my/id/eprint/47379/ Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) Ibrahim, Hayder Hamzah Abba, Yusuf Ahmed, Ihsan Muneer Abdullah, Faez Firdaus Jesse Lim, Eric Teik Chung Marza, Ali Dhiaa Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abd Wahid Mohd Lila, Mohd Azmi Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high mortality rates and indirect effects through impairment of the animal productivity. It is well known that HS affects mainly the respiratory and digestive tracts of host animals. However, involvement of the reproductive system in the pathogenesis of HS has not been reported previously. This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1 × 1012 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 h post-infection, while calves in group 2 were euthanized after day 21 post-infection. Successful isolation and PCR confirmation of P. multocida B:2 was achieved from different parts of the reproductive system, including ovary, oviduct, uterine horn, uterine body and vagina as well as mammary glands and supramammary lymph nodes of the buffaloes in group 1 after 12 h post-infection. However, P. multocida B:2 was not detected in the reproductive organs of buffaloes in group 2 and group 3 after 21 days post-infection. Significant differences (P < 0.05) were found in histopathological changes between the two groups, which mainly affected different anatomic regions of the reproductive system. This work provides an insight into the involvement of the female reproductive system of buffaloes during the pathogenesis of HS and shows that route of inoculation strongly affect the localization of the bacterium in the reproductive system. Springer 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47379/1/Molecular%20detection%20and%20pathology%20of%20Pasteurella%20multocida%202%20in%20the%20reproductive%20system%20of%20pre-pubertal%20buffalo%20calves%20%28Bubalus%20bubalis%29.pdf Ibrahim, Hayder Hamzah and Abba, Yusuf and Ahmed, Ihsan Muneer and Abdullah, Faez Firdaus Jesse and Lim, Eric Teik Chung and Marza, Ali Dhiaa and Saad, Mohd Zamri and Omar, Abdul Rahman and Abu Bakar @ Zakaria, Md Zuki and Saharee, Abdul Aziz and Haron, Abd Wahid and Mohd Lila, Mohd Azmi (2016) Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis). Comparative Clinical Pathology, 25 (2). pp. 319-326. ISSN 1618-5641; ESSN: 1618-565X http://link.springer.com/article/10.1007%2Fs00580-015-2184-y 10.1007/s00580-015-2184-y |
| spellingShingle | Ibrahim, Hayder Hamzah Abba, Yusuf Ahmed, Ihsan Muneer Abdullah, Faez Firdaus Jesse Lim, Eric Teik Chung Marza, Ali Dhiaa Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abd Wahid Mohd Lila, Mohd Azmi Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title | Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title_full | Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title_fullStr | Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title_full_unstemmed | Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title_short | Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis) |
| title_sort | molecular detection and pathology of pasteurella multocida b:2 in the reproductive system of pre-pubertal buffalo calves (bubalus bubalis) |
| url | http://psasir.upm.edu.my/id/eprint/47379/ http://psasir.upm.edu.my/id/eprint/47379/ http://psasir.upm.edu.my/id/eprint/47379/ http://psasir.upm.edu.my/id/eprint/47379/1/Molecular%20detection%20and%20pathology%20of%20Pasteurella%20multocida%202%20in%20the%20reproductive%20system%20of%20pre-pubertal%20buffalo%20calves%20%28Bubalus%20bubalis%29.pdf |