Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was establi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/4709/ http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf |
| _version_ | 1848839896079269888 |
|---|---|
| author | Abdullah, Tosiah Radu, Son Hassan, Zaiton Hashim, Jamal Khair |
| author_facet | Abdullah, Tosiah Radu, Son Hassan, Zaiton Hashim, Jamal Khair |
| author_sort | Abdullah, Tosiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was established for monitoring products derived from GMO that are sold in the markets in Malaysia, which specifically amplify the 35S promoter, nos (nopaline synthase-terminator), EPSPS (5-enolpyruvylshikimate-3-
phosphate synthase) and RRS (CTP/CP4EPSPS). Using this method, we investigated the incidence of genetically modified soy(GM-soy) and specifically the presence of roundup ready soy (RRS). All the soybean samples were evidenced by presence of the lectin gene. Out of 85 samples examined, the 18 positive GM samples were raw bean (9), tofu (8) and tempe (1) (a traditional Malayfood). The results demonstrate for the first time the presence of GM-soy in Malaysian food products, reinforcing the need for the
development of accurate quantitative methods for routine analyses. |
| first_indexed | 2025-11-15T07:18:43Z |
| format | Article |
| id | upm-4709 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:18:43Z |
| publishDate | 2006 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-47092015-12-09T02:56:42Z http://psasir.upm.edu.my/id/eprint/4709/ Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. Abdullah, Tosiah Radu, Son Hassan, Zaiton Hashim, Jamal Khair Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was established for monitoring products derived from GMO that are sold in the markets in Malaysia, which specifically amplify the 35S promoter, nos (nopaline synthase-terminator), EPSPS (5-enolpyruvylshikimate-3- phosphate synthase) and RRS (CTP/CP4EPSPS). Using this method, we investigated the incidence of genetically modified soy(GM-soy) and specifically the presence of roundup ready soy (RRS). All the soybean samples were evidenced by presence of the lectin gene. Out of 85 samples examined, the 18 positive GM samples were raw bean (9), tofu (8) and tempe (1) (a traditional Malayfood). The results demonstrate for the first time the presence of GM-soy in Malaysian food products, reinforcing the need for the development of accurate quantitative methods for routine analyses. Elsevier 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf Abdullah, Tosiah and Radu, Son and Hassan, Zaiton and Hashim, Jamal Khair (2006) Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. Food Chemistry, 98. pp. 575-579. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2005.07.035 10.1016/j.foodchem.2005.07.035 English |
| spellingShingle | Abdullah, Tosiah Radu, Son Hassan, Zaiton Hashim, Jamal Khair Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. |
| title | Detection of genetically modified soy in processed foods
sold commercially in Malaysia by PCR-based method. |
| title_full | Detection of genetically modified soy in processed foods
sold commercially in Malaysia by PCR-based method. |
| title_fullStr | Detection of genetically modified soy in processed foods
sold commercially in Malaysia by PCR-based method. |
| title_full_unstemmed | Detection of genetically modified soy in processed foods
sold commercially in Malaysia by PCR-based method. |
| title_short | Detection of genetically modified soy in processed foods
sold commercially in Malaysia by PCR-based method. |
| title_sort | detection of genetically modified soy in processed foods
sold commercially in malaysia by pcr-based method. |
| url | http://psasir.upm.edu.my/id/eprint/4709/ http://psasir.upm.edu.my/id/eprint/4709/ http://psasir.upm.edu.my/id/eprint/4709/ http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf |