Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa)
Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46734/ http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf |
| _version_ | 1848850727302070272 |
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| author | Wei, Keat Ng Saiful Yazan, Latifah Li, Hua Yap Wan Abd Ghani, Wan Nor Hafiza Chee, Wun How Abdullah, Rasedee |
| author_facet | Wei, Keat Ng Saiful Yazan, Latifah Li, Hua Yap Wan Abd Ghani, Wan Nor Hafiza Chee, Wun How Abdullah, Rasedee |
| author_sort | Wei, Keat Ng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). TQ-NLC was prepared by applying the hot high pressure homogenization technique. The mean particle size of TQ-NLC was 35.66 ± 0.1235 nm with a narrow polydispersity index (PDI) lower than 0.25. The zeta potential of TQ-NLC was greater than −30 mV. Polysorbate 80 helps to increase the stability of TQ-NLC. Differential scanning calorimetry showed that TQ-NLC has a melting point of 56.73°C, which is lower than that of the bulk material. The encapsulation efficiency of TQ in TQ-NLC was 97.63 ± 0.1798% as determined by HPLC analysis. TQ-NLC exhibited antiproliferative activity towards all the cell lines in a dose-dependent manner which was most cytotoxic towards MDA-MB-231 cells. Cell shrinkage was noted following treatment of MDA-MB-231 cells with TQ-NLC with an increase of apoptotic cell population (P < 0.05). TQ-NLC also induced cell cycle arrest. TQ-NLC was most cytotoxic towards MDA-MB-231 cells. It induced apoptosis and cell cycle arrest in the cells. |
| first_indexed | 2025-11-15T10:10:53Z |
| format | Article |
| id | upm-46734 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:10:53Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-467342018-04-23T00:58:52Z http://psasir.upm.edu.my/id/eprint/46734/ Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) Wei, Keat Ng Saiful Yazan, Latifah Li, Hua Yap Wan Abd Ghani, Wan Nor Hafiza Chee, Wun How Abdullah, Rasedee Thymoquinone (TQ) has been shown to exhibit antitumor properties. Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) was developed to improve the bioavailability and cytotoxicity of TQ. This study was conducted to determine the cytotoxic effects of TQ-NLC on breast cancer (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). TQ-NLC was prepared by applying the hot high pressure homogenization technique. The mean particle size of TQ-NLC was 35.66 ± 0.1235 nm with a narrow polydispersity index (PDI) lower than 0.25. The zeta potential of TQ-NLC was greater than −30 mV. Polysorbate 80 helps to increase the stability of TQ-NLC. Differential scanning calorimetry showed that TQ-NLC has a melting point of 56.73°C, which is lower than that of the bulk material. The encapsulation efficiency of TQ in TQ-NLC was 97.63 ± 0.1798% as determined by HPLC analysis. TQ-NLC exhibited antiproliferative activity towards all the cell lines in a dose-dependent manner which was most cytotoxic towards MDA-MB-231 cells. Cell shrinkage was noted following treatment of MDA-MB-231 cells with TQ-NLC with an increase of apoptotic cell population (P < 0.05). TQ-NLC also induced cell cycle arrest. TQ-NLC was most cytotoxic towards MDA-MB-231 cells. It induced apoptosis and cell cycle arrest in the cells. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf Wei, Keat Ng and Saiful Yazan, Latifah and Li, Hua Yap and Wan Abd Ghani, Wan Nor Hafiza and Chee, Wun How and Abdullah, Rasedee (2015) Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa). BioMed Research International. art. no. 263131. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 10.1155/2015/263131 |
| spellingShingle | Wei, Keat Ng Saiful Yazan, Latifah Li, Hua Yap Wan Abd Ghani, Wan Nor Hafiza Chee, Wun How Abdullah, Rasedee Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title | Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title_full | Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title_fullStr | Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title_full_unstemmed | Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title_short | Thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (MDA-MB-231 and MCF-7) and cervical cancer cell lines (HeLa and SiHa) |
| title_sort | thymoquinone-loaded nanostructured lipid carrier exhibited cytotoxicity towards breast cancer cell lines (mda-mb-231 and mcf-7) and cervical cancer cell lines (hela and siha) |
| url | http://psasir.upm.edu.my/id/eprint/46734/ http://psasir.upm.edu.my/id/eprint/46734/ http://psasir.upm.edu.my/id/eprint/46734/1/Thymoquinone-loaded%20nanostructured%20lipid%20carrier%20exhibited%20cytotoxicity%20towards%20breast%20cancer%20cell%20lines%20%28MDA-MB-231%20and%20MCF-7%29%20and%20cervical%20cancer%20cell%20lines%20%28HeLa%20and%20SiHa%29.pdf |