Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities
Plant mediated synthesis of nanoparticles has been considered as green route and a reliable technique for the synthesis of nanoparticles due to its eco-friendly approach. In this study, we report a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using methanolic Mo...
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46459/ http://psasir.upm.edu.my/id/eprint/46459/1/Synthesis%20and%20characterization%20of%20silver%20nanoparticles%20using%20fruit%20extract%20of%20Momordica%20cymbalaria%20and%20assessment%20of%20their%20in%20vitro%20antimicrobial%2C%20antioxidant%20and%20cytotoxicity%20activities.pdf |
| _version_ | 1848850663924039680 |
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| author | Swamy, Mallappa Kumara Akhtar, Mohd. Sayeed Mohanty, Sudipta Kumar Sinniah, Uma Rani |
| author_facet | Swamy, Mallappa Kumara Akhtar, Mohd. Sayeed Mohanty, Sudipta Kumar Sinniah, Uma Rani |
| author_sort | Swamy, Mallappa Kumara |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Plant mediated synthesis of nanoparticles has been considered as green route and a reliable technique for the synthesis of nanoparticles due to its eco-friendly approach. In this study, we report a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using methanolic Momordica cymbalaria fruit extract as reducing agent. The fruit extract of M. cymbalaria exposed to AgNO3 solution showed the change in color from green to light yellow at room temperature within 1 h of incubation confirms the synthesis of AgNPs. UV–vis spectra analysis revealed that the synthesized AgNPs had a sharp surface plasmon resonance at around 450 nm, while, the X-ray Diffraction (XRD) patterns confirmed distinctive peaks indices to the crystalline planes of the face centered cubic silver. The Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) analysis results confirmed the presence of spherical shaped AgNPs by a huge disparity in the particle size distribution with an average size of 15.5 nm. The synthesized AgNPs showed strong antibacterial activity against all the tested multidrug resistant human pathogenic bacterial strains and also exhibited highest free radical scavenging activity (74.2%) compared to fruit extract (60.4%). Moreover, both fruit extract and the synthesized AgNPs showed the cytotoxicity towards Rat L6 skeletal muscle cell line at different concentrations, but the highest inhibition percentage was recorded for AgNPs at concentration of 100 μg/ml. |
| first_indexed | 2025-11-15T10:09:53Z |
| format | Article |
| id | upm-46459 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:09:53Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-464592018-03-27T02:32:58Z http://psasir.upm.edu.my/id/eprint/46459/ Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities Swamy, Mallappa Kumara Akhtar, Mohd. Sayeed Mohanty, Sudipta Kumar Sinniah, Uma Rani Plant mediated synthesis of nanoparticles has been considered as green route and a reliable technique for the synthesis of nanoparticles due to its eco-friendly approach. In this study, we report a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using methanolic Momordica cymbalaria fruit extract as reducing agent. The fruit extract of M. cymbalaria exposed to AgNO3 solution showed the change in color from green to light yellow at room temperature within 1 h of incubation confirms the synthesis of AgNPs. UV–vis spectra analysis revealed that the synthesized AgNPs had a sharp surface plasmon resonance at around 450 nm, while, the X-ray Diffraction (XRD) patterns confirmed distinctive peaks indices to the crystalline planes of the face centered cubic silver. The Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) analysis results confirmed the presence of spherical shaped AgNPs by a huge disparity in the particle size distribution with an average size of 15.5 nm. The synthesized AgNPs showed strong antibacterial activity against all the tested multidrug resistant human pathogenic bacterial strains and also exhibited highest free radical scavenging activity (74.2%) compared to fruit extract (60.4%). Moreover, both fruit extract and the synthesized AgNPs showed the cytotoxicity towards Rat L6 skeletal muscle cell line at different concentrations, but the highest inhibition percentage was recorded for AgNPs at concentration of 100 μg/ml. Elsevier 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46459/1/Synthesis%20and%20characterization%20of%20silver%20nanoparticles%20using%20fruit%20extract%20of%20Momordica%20cymbalaria%20and%20assessment%20of%20their%20in%20vitro%20antimicrobial%2C%20antioxidant%20and%20cytotoxicity%20activities.pdf Swamy, Mallappa Kumara and Akhtar, Mohd. Sayeed and Mohanty, Sudipta Kumar and Sinniah, Uma Rani (2015) Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 151. pp. 939-944. ISSN 1386-1425; ESSN: 1873-3557 10.1016/j.saa.2015.07.009 |
| spellingShingle | Swamy, Mallappa Kumara Akhtar, Mohd. Sayeed Mohanty, Sudipta Kumar Sinniah, Uma Rani Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title | Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title_full | Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title_fullStr | Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title_full_unstemmed | Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title_short | Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| title_sort | synthesis and characterization of silver nanoparticles using fruit extract of momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities |
| url | http://psasir.upm.edu.my/id/eprint/46459/ http://psasir.upm.edu.my/id/eprint/46459/ http://psasir.upm.edu.my/id/eprint/46459/1/Synthesis%20and%20characterization%20of%20silver%20nanoparticles%20using%20fruit%20extract%20of%20Momordica%20cymbalaria%20and%20assessment%20of%20their%20in%20vitro%20antimicrobial%2C%20antioxidant%20and%20cytotoxicity%20activities.pdf |