Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery
Nonviral delivery system receives attention over the last decade. Chitosan (CS) is a cationic polymer whereas saponin (SP) is classified as glycoside. In this study, a spherically-shaped CS-SP nanoparticle was synthesized and characterized. The ability of the nanoparticle to protect DNA from enzymat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46457/ http://psasir.upm.edu.my/id/eprint/46457/1/Synthesis%20and%20characterization%20of%20chitosan-saponin%20nanoparticle%20for%20application%20in%20plasmid%20DNA%20delivery.pdf |
| _version_ | 1848850663419674624 |
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| author | Bande, Faruku Arshad, Siti Suri Mohd Hair Bejo Kamba, Shafiu Abdullahi Omar, Abdul Rahman |
| author_facet | Bande, Faruku Arshad, Siti Suri Mohd Hair Bejo Kamba, Shafiu Abdullahi Omar, Abdul Rahman |
| author_sort | Bande, Faruku |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nonviral delivery system receives attention over the last decade. Chitosan (CS) is a cationic polymer whereas saponin (SP) is classified as glycoside. In this study, a spherically-shaped CS-SP nanoparticle was synthesized and characterized. The ability of the nanoparticle to protect DNA from enzymatic degradation, its thermostability and cytotoxicity were evaluated. The particle size was found below 100 nm as determined by Zetasizer, transmission electron microscopy (TEM), and field scanning electron microscopy (FSEM) results. The surface charge ranges from 43.7 mV to 38.5 mV before and after encapsulation with DNA plasmid, respectively. In terms of thermostability, Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) revealed that CS-SP nanoparticle had a melting temperature of 110°C, with rapid decomposition occurring at 120°C. Encapsulation of DNA with the synthesized nanoparticle was evidenced by changes in the FTIR spectra including characteristic peaks at 3267.39 and 1635.58 cm−1, wavenumbers. Additional peak was also observed at 1169.7 cm−1 following encapsulation. Electrophoretic mobility showed that CS-SP nanoparticle protected plasmid DNA from enzymatic degradation, while cell viability assays confirmed that the synthesized nanoparticle exhibited low cytotoxicity at different concentrations in avian cells. Taken together these, CS-SP nanoparticle showed potentials for applications as a DNA delivery system. |
| first_indexed | 2025-11-15T10:09:52Z |
| format | Article |
| id | upm-46457 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:09:52Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-464572018-03-30T04:17:22Z http://psasir.upm.edu.my/id/eprint/46457/ Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery Bande, Faruku Arshad, Siti Suri Mohd Hair Bejo Kamba, Shafiu Abdullahi Omar, Abdul Rahman Nonviral delivery system receives attention over the last decade. Chitosan (CS) is a cationic polymer whereas saponin (SP) is classified as glycoside. In this study, a spherically-shaped CS-SP nanoparticle was synthesized and characterized. The ability of the nanoparticle to protect DNA from enzymatic degradation, its thermostability and cytotoxicity were evaluated. The particle size was found below 100 nm as determined by Zetasizer, transmission electron microscopy (TEM), and field scanning electron microscopy (FSEM) results. The surface charge ranges from 43.7 mV to 38.5 mV before and after encapsulation with DNA plasmid, respectively. In terms of thermostability, Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) revealed that CS-SP nanoparticle had a melting temperature of 110°C, with rapid decomposition occurring at 120°C. Encapsulation of DNA with the synthesized nanoparticle was evidenced by changes in the FTIR spectra including characteristic peaks at 3267.39 and 1635.58 cm−1, wavenumbers. Additional peak was also observed at 1169.7 cm−1 following encapsulation. Electrophoretic mobility showed that CS-SP nanoparticle protected plasmid DNA from enzymatic degradation, while cell viability assays confirmed that the synthesized nanoparticle exhibited low cytotoxicity at different concentrations in avian cells. Taken together these, CS-SP nanoparticle showed potentials for applications as a DNA delivery system. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46457/1/Synthesis%20and%20characterization%20of%20chitosan-saponin%20nanoparticle%20for%20application%20in%20plasmid%20DNA%20delivery.pdf Bande, Faruku and Arshad, Siti Suri and Mohd Hair Bejo and Kamba, Shafiu Abdullahi and Omar, Abdul Rahman (2015) Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery. Journal of Nanomaterials. art. no. 371529. pp. 1-8. ISSN 1687-4110; ESSN: 1687-4129 10.1155/2015/371529 |
| spellingShingle | Bande, Faruku Arshad, Siti Suri Mohd Hair Bejo Kamba, Shafiu Abdullahi Omar, Abdul Rahman Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title | Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title_full | Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title_fullStr | Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title_full_unstemmed | Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title_short | Synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid DNA delivery |
| title_sort | synthesis and characterization of chitosan-saponin nanoparticle for application in plasmid dna delivery |
| url | http://psasir.upm.edu.my/id/eprint/46457/ http://psasir.upm.edu.my/id/eprint/46457/ http://psasir.upm.edu.my/id/eprint/46457/1/Synthesis%20and%20characterization%20of%20chitosan-saponin%20nanoparticle%20for%20application%20in%20plasmid%20DNA%20delivery.pdf |