Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules
We examine and demonstrate a biosensor using single-mode tapered fiber that has been immobilized with biorecognition molecules to sense targeted proteins. Interaction of evanescent waves with the external medium surrounding the tapered region produces an interferometric-patterned spectrum, which shi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Electrical and Electronics Engineers
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46263/ http://psasir.upm.edu.my/id/eprint/46263/1/Sensitive%20and%20specific%20protein%20sensing%20using%20single-mode%20tapered%20fiber%20immobilized%20with%20biorecognition%20molecules.pdf |
| _version_ | 1848850626780332032 |
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| author | Mustapha Kamil, Y. Abu Bakar, Muhammad Hafiz Mustapa, M. A. Yaacob, Mohd. Hanif Amir Hamzah, Amir Syahir Mahdi, Mohd Adzir |
| author_facet | Mustapha Kamil, Y. Abu Bakar, Muhammad Hafiz Mustapa, M. A. Yaacob, Mohd. Hanif Amir Hamzah, Amir Syahir Mahdi, Mohd Adzir |
| author_sort | Mustapha Kamil, Y. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We examine and demonstrate a biosensor using single-mode tapered fiber that has been immobilized with biorecognition molecules to sense targeted proteins. Interaction of evanescent waves with the external medium surrounding the tapered region produces an interferometric-patterned spectrum, which shifts correspondingly to any changes of refractive index (RI) in the external medium. The proposed setup managed to obtain an RI sensitivity and concentration sensitivity of 2526.8 nm/RIU and 20.368 nm/μM, respectively, which, to our knowledge, is highly sensitive when compared with previous studies. The dynamic performance, good specificity, and high sensitivity of the proposed method highlight an immensely beneficial choice for immunological diagnostics. |
| first_indexed | 2025-11-15T10:09:17Z |
| format | Article |
| id | upm-46263 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:09:17Z |
| publishDate | 2015 |
| publisher | Institute of Electrical and Electronics Engineers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-462632018-05-02T05:09:08Z http://psasir.upm.edu.my/id/eprint/46263/ Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules Mustapha Kamil, Y. Abu Bakar, Muhammad Hafiz Mustapa, M. A. Yaacob, Mohd. Hanif Amir Hamzah, Amir Syahir Mahdi, Mohd Adzir We examine and demonstrate a biosensor using single-mode tapered fiber that has been immobilized with biorecognition molecules to sense targeted proteins. Interaction of evanescent waves with the external medium surrounding the tapered region produces an interferometric-patterned spectrum, which shifts correspondingly to any changes of refractive index (RI) in the external medium. The proposed setup managed to obtain an RI sensitivity and concentration sensitivity of 2526.8 nm/RIU and 20.368 nm/μM, respectively, which, to our knowledge, is highly sensitive when compared with previous studies. The dynamic performance, good specificity, and high sensitivity of the proposed method highlight an immensely beneficial choice for immunological diagnostics. Institute of Electrical and Electronics Engineers 2015-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46263/1/Sensitive%20and%20specific%20protein%20sensing%20using%20single-mode%20tapered%20fiber%20immobilized%20with%20biorecognition%20molecules.pdf Mustapha Kamil, Y. and Abu Bakar, Muhammad Hafiz and Mustapa, M. A. and Yaacob, Mohd. Hanif and Amir Hamzah, Amir Syahir and Mahdi, Mohd Adzir (2015) Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules. IEEE Photonics Journal, 7 (6). art. no. 3700109. pp. 1-9. ISSN 1943-0655 10.1109/JPHOT.2015.2490486 |
| spellingShingle | Mustapha Kamil, Y. Abu Bakar, Muhammad Hafiz Mustapa, M. A. Yaacob, Mohd. Hanif Amir Hamzah, Amir Syahir Mahdi, Mohd Adzir Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title | Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title_full | Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title_fullStr | Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title_full_unstemmed | Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title_short | Sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| title_sort | sensitive and specific protein sensing using single-mode tapered fiber immobilized with biorecognition molecules |
| url | http://psasir.upm.edu.my/id/eprint/46263/ http://psasir.upm.edu.my/id/eprint/46263/ http://psasir.upm.edu.my/id/eprint/46263/1/Sensitive%20and%20specific%20protein%20sensing%20using%20single-mode%20tapered%20fiber%20immobilized%20with%20biorecognition%20molecules.pdf |