Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity
Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature s...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell Publishing
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46232/ http://psasir.upm.edu.my/id/eprint/46232/1/Screening%20of%20various%20parts%20of%20Phaleria%20macrocarpa%20plant%20for%20%CE%B1-glucosidase%20inhibitory%20activity.pdf |
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| author | E., Sabina I. S., M. Zaidul Ghafoor, Kashif M. Jaffri, J. F., Sahena Babiker, E. E. Perumal, V. Mirhosseini, Hamed Mehrnoush, Amid A., Khatib |
| author_facet | E., Sabina I. S., M. Zaidul Ghafoor, Kashif M. Jaffri, J. F., Sahena Babiker, E. E. Perumal, V. Mirhosseini, Hamed Mehrnoush, Amid A., Khatib |
| author_sort | E., Sabina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus. |
| first_indexed | 2025-11-15T10:09:11Z |
| format | Article |
| id | upm-46232 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:09:11Z |
| publishDate | 2016 |
| publisher | Wiley-Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-462322022-06-16T08:23:41Z http://psasir.upm.edu.my/id/eprint/46232/ Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity E., Sabina I. S., M. Zaidul Ghafoor, Kashif M. Jaffri, J. F., Sahena Babiker, E. E. Perumal, V. Mirhosseini, Hamed Mehrnoush, Amid A., Khatib Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus. Wiley-Blackwell Publishing 2016-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46232/1/Screening%20of%20various%20parts%20of%20Phaleria%20macrocarpa%20plant%20for%20%CE%B1-glucosidase%20inhibitory%20activity.pdf E., Sabina and I. S., M. Zaidul and Ghafoor, Kashif and M. Jaffri, J. and F., Sahena and Babiker, E. E. and Perumal, V. and Mirhosseini, Hamed and Mehrnoush, Amid and A., Khatib (2016) Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity. Journal of Food Biochemistry, 40 (2). pp. 201-210. ISSN 0145-8884; ESSN: 1745-4514 https://onlinelibrary.wiley.com/doi/10.1111/jfbc.12212 10.1111/jfbc.12212 |
| spellingShingle | E., Sabina I. S., M. Zaidul Ghafoor, Kashif M. Jaffri, J. F., Sahena Babiker, E. E. Perumal, V. Mirhosseini, Hamed Mehrnoush, Amid A., Khatib Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title | Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title_full | Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title_fullStr | Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title_full_unstemmed | Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title_short | Screening of various parts of Phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| title_sort | screening of various parts of phaleria macrocarpa plant for α-glucosidase inhibitory activity |
| url | http://psasir.upm.edu.my/id/eprint/46232/ http://psasir.upm.edu.my/id/eprint/46232/ http://psasir.upm.edu.my/id/eprint/46232/ http://psasir.upm.edu.my/id/eprint/46232/1/Screening%20of%20various%20parts%20of%20Phaleria%20macrocarpa%20plant%20for%20%CE%B1-glucosidase%20inhibitory%20activity.pdf |