Rat full term amniotic fluid harbors highly potent stem cells
Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46081/ http://psasir.upm.edu.my/id/eprint/46081/1/Rat%20full%20term%20amniotic%20fluid%20harbors%20highly%20potent%20stem%20cells.pdf |
| _version_ | 1848850591186419712 |
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| author | Hoo, Mun-Fun Ferdaos, Nurfarhana Hamzah, Siti Nurusaadah Ridzuan, Noridzzaida Hisham, Nurul Afiqah Abdullah, Syahril Ramasamy, Rajesh Cheah, Pike See Karuppiah, Thilakavathy Yazid, Mohd Nazri Nordin, Norshariza |
| author_facet | Hoo, Mun-Fun Ferdaos, Nurfarhana Hamzah, Siti Nurusaadah Ridzuan, Noridzzaida Hisham, Nurul Afiqah Abdullah, Syahril Ramasamy, Rajesh Cheah, Pike See Karuppiah, Thilakavathy Yazid, Mohd Nazri Nordin, Norshariza |
| author_sort | Hoo, Mun-Fun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we aimed to isolate and characterize stem cells isolated from AF of full term pregnant rats. Three stem cell lines have been established following immuno-selection against the stem cell marker, c-kit. Two of the new lines expressed multiple markers of pluripotency until more than passage 90. Further, they spontaneously differentiated into derivatives of the three primary germ layers through the formation of good quality embryoid bodies (EBs), and can be directly differentiated into neural lineage. Their strong stemness and potent neurogenic properties highlight the presence of highly potent stem cells in AF of full-term pregnancies, which could serve as a potential source of stem cells for regenerative medicine. |
| first_indexed | 2025-11-15T10:08:43Z |
| format | Article |
| id | upm-46081 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:43Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460812022-03-23T04:29:21Z http://psasir.upm.edu.my/id/eprint/46081/ Rat full term amniotic fluid harbors highly potent stem cells Hoo, Mun-Fun Ferdaos, Nurfarhana Hamzah, Siti Nurusaadah Ridzuan, Noridzzaida Hisham, Nurul Afiqah Abdullah, Syahril Ramasamy, Rajesh Cheah, Pike See Karuppiah, Thilakavathy Yazid, Mohd Nazri Nordin, Norshariza Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we aimed to isolate and characterize stem cells isolated from AF of full term pregnant rats. Three stem cell lines have been established following immuno-selection against the stem cell marker, c-kit. Two of the new lines expressed multiple markers of pluripotency until more than passage 90. Further, they spontaneously differentiated into derivatives of the three primary germ layers through the formation of good quality embryoid bodies (EBs), and can be directly differentiated into neural lineage. Their strong stemness and potent neurogenic properties highlight the presence of highly potent stem cells in AF of full-term pregnancies, which could serve as a potential source of stem cells for regenerative medicine. Elsevier 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46081/1/Rat%20full%20term%20amniotic%20fluid%20harbors%20highly%20potent%20stem%20cells.pdf Hoo, Mun-Fun and Ferdaos, Nurfarhana and Hamzah, Siti Nurusaadah and Ridzuan, Noridzzaida and Hisham, Nurul Afiqah and Abdullah, Syahril and Ramasamy, Rajesh and Cheah, Pike See and Karuppiah, Thilakavathy and Yazid, Mohd Nazri and Nordin, Norshariza (2015) Rat full term amniotic fluid harbors highly potent stem cells. Research in Veterinary Science, 102. pp. 89-99. ISSN 0034-5288 https://www.sciencedirect.com/science/article/abs/pii/S0034528815300175?via%3Dihub 10.1016/j.rvsc.2015.07.010 |
| spellingShingle | Hoo, Mun-Fun Ferdaos, Nurfarhana Hamzah, Siti Nurusaadah Ridzuan, Noridzzaida Hisham, Nurul Afiqah Abdullah, Syahril Ramasamy, Rajesh Cheah, Pike See Karuppiah, Thilakavathy Yazid, Mohd Nazri Nordin, Norshariza Rat full term amniotic fluid harbors highly potent stem cells |
| title | Rat full term amniotic fluid harbors highly potent stem cells |
| title_full | Rat full term amniotic fluid harbors highly potent stem cells |
| title_fullStr | Rat full term amniotic fluid harbors highly potent stem cells |
| title_full_unstemmed | Rat full term amniotic fluid harbors highly potent stem cells |
| title_short | Rat full term amniotic fluid harbors highly potent stem cells |
| title_sort | rat full term amniotic fluid harbors highly potent stem cells |
| url | http://psasir.upm.edu.my/id/eprint/46081/ http://psasir.upm.edu.my/id/eprint/46081/ http://psasir.upm.edu.my/id/eprint/46081/ http://psasir.upm.edu.my/id/eprint/46081/1/Rat%20full%20term%20amniotic%20fluid%20harbors%20highly%20potent%20stem%20cells.pdf |