Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract
Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chroma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society of Applied Biology
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46055/ http://psasir.upm.edu.my/id/eprint/46055/1/Qualitative%20phytochemical%20screening%20and%20GC-MS%20profiling%20of%20Azadirachta%20excelsa%20leaf%20extract.pdf |
| _version_ | 1848850586223509504 |
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| author | Shafie, Nurul 'Izzati Samsulrizal, Nurdiana Ahmad Sopian, Nurfarhana Rajion, Mohamed Ali Goh, Yong Meng Md. Ajat, Mohd Mokrish Ahmad, Hafandi |
| author_facet | Shafie, Nurul 'Izzati Samsulrizal, Nurdiana Ahmad Sopian, Nurfarhana Rajion, Mohamed Ali Goh, Yong Meng Md. Ajat, Mohd Mokrish Ahmad, Hafandi |
| author_sort | Shafie, Nurul 'Izzati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chromatography-mass spectrometry (GC-MS). Five grams of leaf extract were extracted using various solvents for phytochemical screening, while another five grams of ethanolic leaf extract was subjected for GC-MS analysis where the mass spectra of the compounds detected were matched with the National Institute of Standards and Technology (NIST) library. The results showed the presence of flavonoids, condensed tannin, triterpenes and steroids in phytochemical screening tests, while GC-MS analysis revealed the presence of seven major compounds in the ethanolic leaf extract. The major component of A.excelsa ethanolic leaf extract was 9, 12, 15-octadecatrienoic acid (42.34%), followed by pentadecanoic acid, 14-methyl-, methyl ester (28.99%), phytol (10.63%), 9, 12, 15-octadecatrien-1-ol (5.37%), octadecanoic acid, methyl ester (4.36%), 9, 12-octadecadienoic acid, methyl ester (4.24%) and hexadecanoic acid, ethyl ester (4.06%). Therefore, the findings of this study form a basis for the characterization of the compounds and allow researchers to investigate the potential of this plant in treating diabetes mellitus. |
| first_indexed | 2025-11-15T10:08:38Z |
| format | Article |
| id | upm-46055 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:38Z |
| publishDate | 2015 |
| publisher | Malaysian Society of Applied Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460552022-03-28T07:11:33Z http://psasir.upm.edu.my/id/eprint/46055/ Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract Shafie, Nurul 'Izzati Samsulrizal, Nurdiana Ahmad Sopian, Nurfarhana Rajion, Mohamed Ali Goh, Yong Meng Md. Ajat, Mohd Mokrish Ahmad, Hafandi Azadirachta excelsa is traditionally used by the locals to treat diabetes mellitus but the active compounds of the leaves have not been identified yet. Hence, the aim of the study was to identify the components of Azadirachta excelsa leaf extract by qualitative phytochemical screening and gas chromatography-mass spectrometry (GC-MS). Five grams of leaf extract were extracted using various solvents for phytochemical screening, while another five grams of ethanolic leaf extract was subjected for GC-MS analysis where the mass spectra of the compounds detected were matched with the National Institute of Standards and Technology (NIST) library. The results showed the presence of flavonoids, condensed tannin, triterpenes and steroids in phytochemical screening tests, while GC-MS analysis revealed the presence of seven major compounds in the ethanolic leaf extract. The major component of A.excelsa ethanolic leaf extract was 9, 12, 15-octadecatrienoic acid (42.34%), followed by pentadecanoic acid, 14-methyl-, methyl ester (28.99%), phytol (10.63%), 9, 12, 15-octadecatrien-1-ol (5.37%), octadecanoic acid, methyl ester (4.36%), 9, 12-octadecadienoic acid, methyl ester (4.24%) and hexadecanoic acid, ethyl ester (4.06%). Therefore, the findings of this study form a basis for the characterization of the compounds and allow researchers to investigate the potential of this plant in treating diabetes mellitus. Malaysian Society of Applied Biology 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46055/1/Qualitative%20phytochemical%20screening%20and%20GC-MS%20profiling%20of%20Azadirachta%20excelsa%20leaf%20extract.pdf Shafie, Nurul 'Izzati and Samsulrizal, Nurdiana and Ahmad Sopian, Nurfarhana and Rajion, Mohamed Ali and Goh, Yong Meng and Md. Ajat, Mohd Mokrish and Ahmad, Hafandi (2015) Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract. Malaysian Applied Biology, 44 (3). pp. 87-92. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/article/view/2023 |
| spellingShingle | Shafie, Nurul 'Izzati Samsulrizal, Nurdiana Ahmad Sopian, Nurfarhana Rajion, Mohamed Ali Goh, Yong Meng Md. Ajat, Mohd Mokrish Ahmad, Hafandi Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title | Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title_full | Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title_fullStr | Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title_full_unstemmed | Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title_short | Qualitative phytochemical screening and GC-MS profiling of Azadirachta excelsa leaf extract |
| title_sort | qualitative phytochemical screening and gc-ms profiling of azadirachta excelsa leaf extract |
| url | http://psasir.upm.edu.my/id/eprint/46055/ http://psasir.upm.edu.my/id/eprint/46055/ http://psasir.upm.edu.my/id/eprint/46055/1/Qualitative%20phytochemical%20screening%20and%20GC-MS%20profiling%20of%20Azadirachta%20excelsa%20leaf%20extract.pdf |