Protein identification of vitellogenin in river catfish (Hemibagrus nemurus)
Mass production of fish broodstock with high quality eggs requires the knowledge on the chemical composition and physiochemical properties of vitellogenin (Vtg) during ovulation. Vtg is an egg yolk precursor phospholipoglycoprotein, and has been analysed to evaluate the reproductive conditions and d...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Open Journal Systems Public Knowledge Project
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/46034/ http://psasir.upm.edu.my/id/eprint/46034/1/Protein%20identification%20of%20vitellogenin%20in%20river%20catfish%20%28Hemibagrus%20nemurus%29.pdf |
| _version_ | 1848850582179151872 |
|---|---|
| author | Othman, Roshani Harmin, Sharr Azni Md Yasin, Ina-Salwany |
| author_facet | Othman, Roshani Harmin, Sharr Azni Md Yasin, Ina-Salwany |
| author_sort | Othman, Roshani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mass production of fish broodstock with high quality eggs requires the knowledge on the chemical composition and physiochemical properties of vitellogenin (Vtg) during ovulation. Vtg is an egg yolk precursor phospholipoglycoprotein, and has been analysed to evaluate the reproductive conditions and determine the spawning period in captive and wild fish. In this study, Vtg was induced in male H. nemurus through three intramuscular injections of 17- estradiol (E2). The Vtg was purified from the serum using gel filtration chromatography and thepurified protein was reduced via SDS-PAGE. One major polypeptide corresponding to 130 kDa was observed. Vtg identification was done using peptide mass fingerprint (PMF) from the trypsin digestion of male H. nemurus Vtg induced with E2. The sequence homology of H. nemurus AYLAGAAADVLEVGVR matched the Vtg of other fish species when analysedusing MALDI-TOF. Vtg was confirmed by MASCOT at 95% significant level. The potential protein that controls the reproductive process and oocyte development isolated from this study was discussed to understand the structure and function of Vtg. |
| first_indexed | 2025-11-15T10:08:35Z |
| format | Article |
| id | upm-46034 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:35Z |
| publishDate | 2015 |
| publisher | Open Journal Systems Public Knowledge Project |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460342018-05-14T00:52:40Z http://psasir.upm.edu.my/id/eprint/46034/ Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) Othman, Roshani Harmin, Sharr Azni Md Yasin, Ina-Salwany Mass production of fish broodstock with high quality eggs requires the knowledge on the chemical composition and physiochemical properties of vitellogenin (Vtg) during ovulation. Vtg is an egg yolk precursor phospholipoglycoprotein, and has been analysed to evaluate the reproductive conditions and determine the spawning period in captive and wild fish. In this study, Vtg was induced in male H. nemurus through three intramuscular injections of 17- estradiol (E2). The Vtg was purified from the serum using gel filtration chromatography and thepurified protein was reduced via SDS-PAGE. One major polypeptide corresponding to 130 kDa was observed. Vtg identification was done using peptide mass fingerprint (PMF) from the trypsin digestion of male H. nemurus Vtg induced with E2. The sequence homology of H. nemurus AYLAGAAADVLEVGVR matched the Vtg of other fish species when analysedusing MALDI-TOF. Vtg was confirmed by MASCOT at 95% significant level. The potential protein that controls the reproductive process and oocyte development isolated from this study was discussed to understand the structure and function of Vtg. Open Journal Systems Public Knowledge Project 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46034/1/Protein%20identification%20of%20vitellogenin%20in%20river%20catfish%20%28Hemibagrus%20nemurus%29.pdf Othman, Roshani and Harmin, Sharr Azni and Md Yasin, Ina-Salwany (2015) Protein identification of vitellogenin in river catfish (Hemibagrus nemurus). Bioremediation Science & Technology Research, 3 (1). pp. 1-5. ISSN 2289-5892 http://journal.hibiscuspublisher.com/index .php/BSTR |
| spellingShingle | Othman, Roshani Harmin, Sharr Azni Md Yasin, Ina-Salwany Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title | Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title_full | Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title_fullStr | Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title_full_unstemmed | Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title_short | Protein identification of vitellogenin in river catfish (Hemibagrus nemurus) |
| title_sort | protein identification of vitellogenin in river catfish (hemibagrus nemurus) |
| url | http://psasir.upm.edu.my/id/eprint/46034/ http://psasir.upm.edu.my/id/eprint/46034/ http://psasir.upm.edu.my/id/eprint/46034/1/Protein%20identification%20of%20vitellogenin%20in%20river%20catfish%20%28Hemibagrus%20nemurus%29.pdf |