Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968
Fucoxanthin is the major carotenoid produced by diatoms and shown to exhibit potent anti-obesity and anti-cancer activities. This study presents a simple protocol for the preparation of fucoxanthin-rich fraction (FxRF) with high antioxidative properties from a selected marine diatom, Chaetoceros cal...
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46012/ http://psasir.upm.edu.my/id/eprint/46012/1/Production%20of%20fucoxanthin-rich%20fraction%20%28FxRF%29%20from%20a%20diatom%2C%20Chaetoceros%20calcitrans%20%28Paulsen%29%20Takano%201968.pdf |
| _version_ | 1848850578323537920 |
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| author | Foo, Su Chern Md. Yusoff, Fatimah Ismail, Maznah Basri, Mahiran Chan, Kim Wei Khong, Nicholas Mun Hoe Yau, Sook Kun |
| author_facet | Foo, Su Chern Md. Yusoff, Fatimah Ismail, Maznah Basri, Mahiran Chan, Kim Wei Khong, Nicholas Mun Hoe Yau, Sook Kun |
| author_sort | Foo, Su Chern |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fucoxanthin is the major carotenoid produced by diatoms and shown to exhibit potent anti-obesity and anti-cancer activities. This study presents a simple protocol for the preparation of fucoxanthin-rich fraction (FxRF) with high antioxidative properties from a selected marine diatom, Chaetoceros calcitrans. Fucoxanthin concentrates were prepared from crude methanolic extract (CME) with liquid–liquid partitioning of three types of solvent systems (i.e. diethyl ether–water, dichloromethane–water and ethyl acetate–water), followed by comparative antioxidant evaluation. Dichloromethane fraction (DCMF) was found to contain significantly higher (p < 0.05) level of carotenoid (7.13 ± 0.01 mg·g− 1 DW) and fucoxanthin (5.25 ± 0.03 mg FX·g− 1 DW) contents accompanied with elevated (p < 0.05) antioxidant activities (DPPHradical dot and ABTSradical dot+ radical scavenging, and beta carotene bleaching assays) as compared to other tested fractions. This study showed that FxRF with enhanced antioxidant properties could be effectively produced and concentrated through the DCM–H2O partition of CME. |
| first_indexed | 2025-11-15T10:08:31Z |
| format | Article |
| id | upm-46012 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:31Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460122016-06-07T08:18:08Z http://psasir.upm.edu.my/id/eprint/46012/ Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 Foo, Su Chern Md. Yusoff, Fatimah Ismail, Maznah Basri, Mahiran Chan, Kim Wei Khong, Nicholas Mun Hoe Yau, Sook Kun Fucoxanthin is the major carotenoid produced by diatoms and shown to exhibit potent anti-obesity and anti-cancer activities. This study presents a simple protocol for the preparation of fucoxanthin-rich fraction (FxRF) with high antioxidative properties from a selected marine diatom, Chaetoceros calcitrans. Fucoxanthin concentrates were prepared from crude methanolic extract (CME) with liquid–liquid partitioning of three types of solvent systems (i.e. diethyl ether–water, dichloromethane–water and ethyl acetate–water), followed by comparative antioxidant evaluation. Dichloromethane fraction (DCMF) was found to contain significantly higher (p < 0.05) level of carotenoid (7.13 ± 0.01 mg·g− 1 DW) and fucoxanthin (5.25 ± 0.03 mg FX·g− 1 DW) contents accompanied with elevated (p < 0.05) antioxidant activities (DPPHradical dot and ABTSradical dot+ radical scavenging, and beta carotene bleaching assays) as compared to other tested fractions. This study showed that FxRF with enhanced antioxidant properties could be effectively produced and concentrated through the DCM–H2O partition of CME. Elsevier 2015-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/46012/1/Production%20of%20fucoxanthin-rich%20fraction%20%28FxRF%29%20from%20a%20diatom%2C%20Chaetoceros%20calcitrans%20%28Paulsen%29%20Takano%201968.pdf Foo, Su Chern and Md. Yusoff, Fatimah and Ismail, Maznah and Basri, Mahiran and Chan, Kim Wei and Khong, Nicholas Mun Hoe and Yau, Sook Kun (2015) Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968. Algal Research, 12. pp. 26-32. ISSN 2211-9264 http://www.sciencedirect.com/science/article/pii/S2211926415300321 10.1016/j.algal.2015.08.004 |
| spellingShingle | Foo, Su Chern Md. Yusoff, Fatimah Ismail, Maznah Basri, Mahiran Chan, Kim Wei Khong, Nicholas Mun Hoe Yau, Sook Kun Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title | Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title_full | Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title_fullStr | Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title_full_unstemmed | Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title_short | Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968 |
| title_sort | production of fucoxanthin-rich fraction (fxrf) from a diatom, chaetoceros calcitrans (paulsen) takano 1968 |
| url | http://psasir.upm.edu.my/id/eprint/46012/ http://psasir.upm.edu.my/id/eprint/46012/ http://psasir.upm.edu.my/id/eprint/46012/ http://psasir.upm.edu.my/id/eprint/46012/1/Production%20of%20fucoxanthin-rich%20fraction%20%28FxRF%29%20from%20a%20diatom%2C%20Chaetoceros%20calcitrans%20%28Paulsen%29%20Takano%201968.pdf |