Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy
Probiotics are live microorganisms that exert beneficial effects on the host, when administered in adequate amounts. Mostly, probiotics affect the gastrointestinal (GI) tract of the host and alter the composition of gut microbiota. Nowadays, the incidence of hip fractures due to osteoporosis is incr...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46009/ http://psasir.upm.edu.my/id/eprint/46009/1/Probiotics%20%28Bifidobacterium%20longum%29%20increase%20bone%20mass%20density%20and%20upregulate%20sparc%20and%20Bmp-2%20genes%20in%20rats%20with%20bone%20loss%20resulting%20from%20ovariectomy.pdf |
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| author | Parvaneh, Kolsoom Ebrahimi, Mahdi Sabran, Mohd Redzwan Karimi, Golgis Ng, Angela Min Hwei Abdul-Majeed, Saif Ahmad, Zuraini Ibrahim, Zuriati Jamaluddin, Rosita |
| author_facet | Parvaneh, Kolsoom Ebrahimi, Mahdi Sabran, Mohd Redzwan Karimi, Golgis Ng, Angela Min Hwei Abdul-Majeed, Saif Ahmad, Zuraini Ibrahim, Zuriati Jamaluddin, Rosita |
| author_sort | Parvaneh, Kolsoom |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Probiotics are live microorganisms that exert beneficial effects on the host, when administered in adequate amounts. Mostly, probiotics affect the gastrointestinal (GI) tract of the host and alter the composition of gut microbiota. Nowadays, the incidence of hip fractures due to osteoporosis is increasing worldwide. Ovariectomized (OVX) rats have fragile bone due to estrogen deficiency and mimic the menopausal conditions in women. Therefore, this study aimed to examine the effects of Bifidobacterium longum (B. longum) on bone mass density (BMD), bone mineral content (BMC), bone remodeling, bone structure, and gene expression in OVX rats. The rats were randomly assigned into 3 groups (sham, OVX, and the OVX group supplemented with 1 mL of B. longum 108–109 colony forming units (CFU)/mL). B. longum was given once daily for 16 weeks, starting from 2 weeks after the surgery. The B. longum supplementation increased (p < 0.05) serum osteocalcin (OC) and osteoblasts, bone formation parameters, and decreased serum C-terminal telopeptide (CTX) and osteoclasts, bone resorption parameters. It also altered the microstructure of the femur. Consequently, it increased BMD by increasing (p < 0.05) the expression of Sparc and Bmp-2 genes. B. longum alleviated bone loss in OVX rats and enhanced BMD by decreasing bone resorption and increasing bone formation. |
| first_indexed | 2025-11-15T10:08:30Z |
| format | Article |
| id | upm-46009 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:30Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460092018-05-14T08:37:35Z http://psasir.upm.edu.my/id/eprint/46009/ Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy Parvaneh, Kolsoom Ebrahimi, Mahdi Sabran, Mohd Redzwan Karimi, Golgis Ng, Angela Min Hwei Abdul-Majeed, Saif Ahmad, Zuraini Ibrahim, Zuriati Jamaluddin, Rosita Probiotics are live microorganisms that exert beneficial effects on the host, when administered in adequate amounts. Mostly, probiotics affect the gastrointestinal (GI) tract of the host and alter the composition of gut microbiota. Nowadays, the incidence of hip fractures due to osteoporosis is increasing worldwide. Ovariectomized (OVX) rats have fragile bone due to estrogen deficiency and mimic the menopausal conditions in women. Therefore, this study aimed to examine the effects of Bifidobacterium longum (B. longum) on bone mass density (BMD), bone mineral content (BMC), bone remodeling, bone structure, and gene expression in OVX rats. The rats were randomly assigned into 3 groups (sham, OVX, and the OVX group supplemented with 1 mL of B. longum 108–109 colony forming units (CFU)/mL). B. longum was given once daily for 16 weeks, starting from 2 weeks after the surgery. The B. longum supplementation increased (p < 0.05) serum osteocalcin (OC) and osteoblasts, bone formation parameters, and decreased serum C-terminal telopeptide (CTX) and osteoclasts, bone resorption parameters. It also altered the microstructure of the femur. Consequently, it increased BMD by increasing (p < 0.05) the expression of Sparc and Bmp-2 genes. B. longum alleviated bone loss in OVX rats and enhanced BMD by decreasing bone resorption and increasing bone formation. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46009/1/Probiotics%20%28Bifidobacterium%20longum%29%20increase%20bone%20mass%20density%20and%20upregulate%20sparc%20and%20Bmp-2%20genes%20in%20rats%20with%20bone%20loss%20resulting%20from%20ovariectomy.pdf Parvaneh, Kolsoom and Ebrahimi, Mahdi and Sabran, Mohd Redzwan and Karimi, Golgis and Ng, Angela Min Hwei and Abdul-Majeed, Saif and Ahmad, Zuraini and Ibrahim, Zuriati and Jamaluddin, Rosita (2015) Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy. BioMed Research International, 2015. art. no. 897639. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 10.1155/2015/897639 |
| spellingShingle | Parvaneh, Kolsoom Ebrahimi, Mahdi Sabran, Mohd Redzwan Karimi, Golgis Ng, Angela Min Hwei Abdul-Majeed, Saif Ahmad, Zuraini Ibrahim, Zuriati Jamaluddin, Rosita Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title | Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title_full | Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title_fullStr | Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title_full_unstemmed | Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title_short | Probiotics (Bifidobacterium longum) increase bone mass density and upregulate sparc and Bmp-2 genes in rats with bone loss resulting from ovariectomy |
| title_sort | probiotics (bifidobacterium longum) increase bone mass density and upregulate sparc and bmp-2 genes in rats with bone loss resulting from ovariectomy |
| url | http://psasir.upm.edu.my/id/eprint/46009/ http://psasir.upm.edu.my/id/eprint/46009/ http://psasir.upm.edu.my/id/eprint/46009/1/Probiotics%20%28Bifidobacterium%20longum%29%20increase%20bone%20mass%20density%20and%20upregulate%20sparc%20and%20Bmp-2%20genes%20in%20rats%20with%20bone%20loss%20resulting%20from%20ovariectomy.pdf |