Optimization of conditions for the single step IMAC purification of miraculin from Synsepalum dulcificum

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Optimization of conditions for the single step IMAC purification of miraculin from Synsepalum dulcificum

In this study, the methods for extraction and purification of miraculin from Synsepalum dulcificum were investigated. For extraction, the effect of different extraction buffers (phosphate buffer saline, Tris-HCl and NaCl) on the extraction efficiency of total protein was evaluated. Immobilized metal...

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Bibliographic Details
Main Authors:	Ariff, Arbakariya, Lai, Oi Ming, Joo, Shun Tan, He, Zu Xing
Format:	Article
Language:	English
Published:	Elsevier 2015
Online Access:	http://psasir.upm.edu.my/id/eprint/45693/ http://psasir.upm.edu.my/id/eprint/45693/1/IMAC.pdf

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