Molecular identification and composition of cyclorrhaphan flies associated with cafeterias

Cafeterias are routinely visited by people to fulfil their daily feeding and drinking requirements. Unnoticed visitors, such as cyclorrhaphan flies, are also present in these places which can be a source of food poisoning and disease spread. These flies were collected from garbage piles, kitchen and...

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Main Authors: Khoso, Fahad Nazir, Tan, Marianne Pueh Im, Talib, Siti Mahsuri, Lau, Wei Hong
Format: Article
Language:English
Published: Smith and Franklin Academic Publishing 2015
Online Access:http://psasir.upm.edu.my/id/eprint/45527/
http://psasir.upm.edu.my/id/eprint/45527/1/CAFE.pdf
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author Khoso, Fahad Nazir
Tan, Marianne Pueh Im
Talib, Siti Mahsuri
Lau, Wei Hong
author_facet Khoso, Fahad Nazir
Tan, Marianne Pueh Im
Talib, Siti Mahsuri
Lau, Wei Hong
author_sort Khoso, Fahad Nazir
building UPM Institutional Repository
collection Online Access
description Cafeterias are routinely visited by people to fulfil their daily feeding and drinking requirements. Unnoticed visitors, such as cyclorrhaphan flies, are also present in these places which can be a source of food poisoning and disease spread. These flies were collected from garbage piles, kitchen and vacant sites of two cafeterias (Cafeteria Serumpun and Cafeteria Agro-bio) within Universiti Putra Malaysia and one cafeteria (Cafeteria Old-flat) outside the university. A total of 1,037 fly specimens were collected and identified belonging to Calliphoridae, Muscidae and Sarcophagadae. The COI and COII gene sequences and phylogenetic results revealed five species of cyclorrhaphan flies, namely Chrysomya megacephala, Lucilia cuprina, Musca domestica, Ophyra spinigera and Sarcophaga dux. The highest number of flies was found at Cafeteria Serumpun (44%), followed by Cafeteria Old-flat (36%) and Cafeteria Agro-bio (20%). The most populated sampling site was kitchen and the abundant species was C. megacephala (92.66%). Analysis of data showed significant difference between individuals of different species at different cafeterias and sampling sites.
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spelling upm-455272021-04-08T02:36:51Z http://psasir.upm.edu.my/id/eprint/45527/ Molecular identification and composition of cyclorrhaphan flies associated with cafeterias Khoso, Fahad Nazir Tan, Marianne Pueh Im Talib, Siti Mahsuri Lau, Wei Hong Cafeterias are routinely visited by people to fulfil their daily feeding and drinking requirements. Unnoticed visitors, such as cyclorrhaphan flies, are also present in these places which can be a source of food poisoning and disease spread. These flies were collected from garbage piles, kitchen and vacant sites of two cafeterias (Cafeteria Serumpun and Cafeteria Agro-bio) within Universiti Putra Malaysia and one cafeteria (Cafeteria Old-flat) outside the university. A total of 1,037 fly specimens were collected and identified belonging to Calliphoridae, Muscidae and Sarcophagadae. The COI and COII gene sequences and phylogenetic results revealed five species of cyclorrhaphan flies, namely Chrysomya megacephala, Lucilia cuprina, Musca domestica, Ophyra spinigera and Sarcophaga dux. The highest number of flies was found at Cafeteria Serumpun (44%), followed by Cafeteria Old-flat (36%) and Cafeteria Agro-bio (20%). The most populated sampling site was kitchen and the abundant species was C. megacephala (92.66%). Analysis of data showed significant difference between individuals of different species at different cafeterias and sampling sites. Smith and Franklin Academic Publishing 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45527/1/CAFE.pdf Khoso, Fahad Nazir and Tan, Marianne Pueh Im and Talib, Siti Mahsuri and Lau, Wei Hong (2015) Molecular identification and composition of cyclorrhaphan flies associated with cafeterias. Pakistan Journal of Zoology, 47 (6). pp. 1743-1752. ISSN 0030-9923 http://www.zsp.com.pk/pdf47/1743-1752%20(32)%20QPJZ-0111-2015%202-10-15%20Revised.pdf
spellingShingle Khoso, Fahad Nazir
Tan, Marianne Pueh Im
Talib, Siti Mahsuri
Lau, Wei Hong
Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title_full Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title_fullStr Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title_full_unstemmed Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title_short Molecular identification and composition of cyclorrhaphan flies associated with cafeterias
title_sort molecular identification and composition of cyclorrhaphan flies associated with cafeterias
url http://psasir.upm.edu.my/id/eprint/45527/
http://psasir.upm.edu.my/id/eprint/45527/
http://psasir.upm.edu.my/id/eprint/45527/1/CAFE.pdf