Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice

The study was undertaken to characterize plant growth promoting rhizobacteria (PGPR) for growth enhancement and stress tolerant traits and their efficacy on early establishment of rice seedling. In vitro growth promoting traits revealed that out of 30 PGPR isolates, 18 fixed nitrogen, 17 solubilized...

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Main Authors: Habib, Sheikh Hasna, Kausar, Hossain, Mohd Saud, Halimi, Ismail, Mohd Razi, Othman, Radziah
Format: Article
Language:English
Published: Friends Science Publishers 2015
Online Access:http://psasir.upm.edu.my/id/eprint/45520/
http://psasir.upm.edu.my/id/eprint/45520/1/ORYZA.pdf
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author Habib, Sheikh Hasna
Kausar, Hossain
Mohd Saud, Halimi
Ismail, Mohd Razi
Othman, Radziah
author_facet Habib, Sheikh Hasna
Kausar, Hossain
Mohd Saud, Halimi
Ismail, Mohd Razi
Othman, Radziah
author_sort Habib, Sheikh Hasna
building UPM Institutional Repository
collection Online Access
description The study was undertaken to characterize plant growth promoting rhizobacteria (PGPR) for growth enhancement and stress tolerant traits and their efficacy on early establishment of rice seedling. In vitro growth promoting traits revealed that out of 30 PGPR isolates, 18 fixed nitrogen, 17 solubilized tri-calcium phosphate, 29 and 17 produced IAA with or without addition of L-tryptophane.In case of stree tolerant activities, PGPR isolates tolerated pH ranging from 5 to 10, Nacl from 1 to 6% and polyethylene glycol (PEG) from 10 to 40%, respectively. They showed antagonistic activity against Pyricularia oryzae with PIRG values ranging from 7−68%. After two-stage of screening, isolates UPMR7 and UPMR17 were identified based on 16s rRNA gene sequences and matched to the genus Bacillus and Citrobacter with 97−98% similarity. UPMR 7 and UPMR 17 were further evaluated on early growth promotion of rice variety MR219. Results revealed that PGPR inoculation had significant effects on plant growth compared to non-inoculated plants. Thus, it could be suggested that the isolates UPMR7 and UPMR17 have the potential to be used as biofertilizer and bioenhancer in sustainable rice cultivation.
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spelling upm-455202021-01-23T21:57:00Z http://psasir.upm.edu.my/id/eprint/45520/ Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice Habib, Sheikh Hasna Kausar, Hossain Mohd Saud, Halimi Ismail, Mohd Razi Othman, Radziah The study was undertaken to characterize plant growth promoting rhizobacteria (PGPR) for growth enhancement and stress tolerant traits and their efficacy on early establishment of rice seedling. In vitro growth promoting traits revealed that out of 30 PGPR isolates, 18 fixed nitrogen, 17 solubilized tri-calcium phosphate, 29 and 17 produced IAA with or without addition of L-tryptophane.In case of stree tolerant activities, PGPR isolates tolerated pH ranging from 5 to 10, Nacl from 1 to 6% and polyethylene glycol (PEG) from 10 to 40%, respectively. They showed antagonistic activity against Pyricularia oryzae with PIRG values ranging from 7−68%. After two-stage of screening, isolates UPMR7 and UPMR17 were identified based on 16s rRNA gene sequences and matched to the genus Bacillus and Citrobacter with 97−98% similarity. UPMR 7 and UPMR 17 were further evaluated on early growth promotion of rice variety MR219. Results revealed that PGPR inoculation had significant effects on plant growth compared to non-inoculated plants. Thus, it could be suggested that the isolates UPMR7 and UPMR17 have the potential to be used as biofertilizer and bioenhancer in sustainable rice cultivation. Friends Science Publishers 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45520/1/ORYZA.pdf Habib, Sheikh Hasna and Kausar, Hossain and Mohd Saud, Halimi and Ismail, Mohd Razi and Othman, Radziah (2015) Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice. International Journal of Agriculture & Biology. pp. 1-8. ISSN 1560-8530; ESSN: 1814-9596 https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.730.7185&rep=rep1&type=pdf 10.17957/IJAB/15.0094
spellingShingle Habib, Sheikh Hasna
Kausar, Hossain
Mohd Saud, Halimi
Ismail, Mohd Razi
Othman, Radziah
Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title_full Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title_fullStr Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title_full_unstemmed Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title_short Molecular characterization of stress tolerant plant growth promoting rhizobacteria (PGPR) for growth enhancement of rice
title_sort molecular characterization of stress tolerant plant growth promoting rhizobacteria (pgpr) for growth enhancement of rice
url http://psasir.upm.edu.my/id/eprint/45520/
http://psasir.upm.edu.my/id/eprint/45520/
http://psasir.upm.edu.my/id/eprint/45520/
http://psasir.upm.edu.my/id/eprint/45520/1/ORYZA.pdf