Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment
Objectives: Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages. M...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/45078/ http://psasir.upm.edu.my/id/eprint/45078/1/Improved%20functional%20assessment%20of%20osteoarthritic%20knee%20joint%20after%20chondrogenically%20induced%20cell%20treatment.pdf |
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| author | Ude, Chinedu C. Ng, Min Hwei Chen, Hui Cheng Htwe, Ohnmar Amaramalar, Naicker Sylva Hassan, Md. Shariful Djordjevic, Ivan Abdul Rani, Rizal Ahmad, Johan Mohammad Yahya, Norhamdan Saim, Aminuddin Idrus, Ruszymah |
| author_facet | Ude, Chinedu C. Ng, Min Hwei Chen, Hui Cheng Htwe, Ohnmar Amaramalar, Naicker Sylva Hassan, Md. Shariful Djordjevic, Ivan Abdul Rani, Rizal Ahmad, Johan Mohammad Yahya, Norhamdan Saim, Aminuddin Idrus, Ruszymah |
| author_sort | Ude, Chinedu C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objectives: Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages. Methods: Baseline electromyogram (EMGs) were conducted at week 0 (pre-OA), on the locomotory muscles of nine un-castrated male sheep (Siamese long tail cross) divided into controls, adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs), before OA inductions. Subsequent recordings were performed at week 7 and week 31 which were post-OA and post-treatments. Afterwards, the compression tests of the regenerated cartilage were performed. Results: Post-treatment EMG analysis revealed that the control sheep retained significant reductions in amplitudes at the right medial gluteus, vastus lateralis and bicep femoris, whereas BMSCs and ADSCs samples had no further significant reductions (P < 0.05). Grossly and histologically, the treated knee joints demonstrated the presence of regenerated neo cartilages evidenced by the fluorescence of PKH26 tracker. Based on the International Cartilage Repair Society scores (ICRS), they had significantly lower grades than the controls (P < 0.05). The compression moduli of the native cartilages and the engineered cartilages differed significantly at the tibia plateau, patella femoral groove and the patella; whereas at the medial femoral condyle, they had similar moduli of 0.69 MPa and 0.40-0.64 MPa respectively. Their compression strengths at all four regions were within ±10 MPa.
Conclusion: The tissue engineered cartilages provided evidence of functional recoveries associated to the structural regenerations, and their mechanical properties were comparable with the native cartilage. |
| first_indexed | 2025-11-15T10:06:55Z |
| format | Article |
| id | upm-45078 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:06:55Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-450782021-04-18T00:37:03Z http://psasir.upm.edu.my/id/eprint/45078/ Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment Ude, Chinedu C. Ng, Min Hwei Chen, Hui Cheng Htwe, Ohnmar Amaramalar, Naicker Sylva Hassan, Md. Shariful Djordjevic, Ivan Abdul Rani, Rizal Ahmad, Johan Mohammad Yahya, Norhamdan Saim, Aminuddin Idrus, Ruszymah Objectives: Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages. Methods: Baseline electromyogram (EMGs) were conducted at week 0 (pre-OA), on the locomotory muscles of nine un-castrated male sheep (Siamese long tail cross) divided into controls, adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs), before OA inductions. Subsequent recordings were performed at week 7 and week 31 which were post-OA and post-treatments. Afterwards, the compression tests of the regenerated cartilage were performed. Results: Post-treatment EMG analysis revealed that the control sheep retained significant reductions in amplitudes at the right medial gluteus, vastus lateralis and bicep femoris, whereas BMSCs and ADSCs samples had no further significant reductions (P < 0.05). Grossly and histologically, the treated knee joints demonstrated the presence of regenerated neo cartilages evidenced by the fluorescence of PKH26 tracker. Based on the International Cartilage Repair Society scores (ICRS), they had significantly lower grades than the controls (P < 0.05). The compression moduli of the native cartilages and the engineered cartilages differed significantly at the tibia plateau, patella femoral groove and the patella; whereas at the medial femoral condyle, they had similar moduli of 0.69 MPa and 0.40-0.64 MPa respectively. Their compression strengths at all four regions were within ±10 MPa. Conclusion: The tissue engineered cartilages provided evidence of functional recoveries associated to the structural regenerations, and their mechanical properties were comparable with the native cartilage. Elsevier 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45078/1/Improved%20functional%20assessment%20of%20osteoarthritic%20knee%20joint%20after%20chondrogenically%20induced%20cell%20treatment.pdf Ude, Chinedu C. and Ng, Min Hwei and Chen, Hui Cheng and Htwe, Ohnmar and Amaramalar, Naicker Sylva and Hassan, Md. Shariful and Djordjevic, Ivan and Abdul Rani, Rizal and Ahmad, Johan and Mohammad Yahya, Norhamdan and Saim, Aminuddin and Idrus, Ruszymah (2015) Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment. Osteoarthritis and Cartilage, 23 (8). pp. 1294-1306. ISSN 1063-4584; ESSN:1522-9653 https://pubmed.ncbi.nlm.nih.gov/25887366/ 10.1016/j.joca.2015.04.003 |
| spellingShingle | Ude, Chinedu C. Ng, Min Hwei Chen, Hui Cheng Htwe, Ohnmar Amaramalar, Naicker Sylva Hassan, Md. Shariful Djordjevic, Ivan Abdul Rani, Rizal Ahmad, Johan Mohammad Yahya, Norhamdan Saim, Aminuddin Idrus, Ruszymah Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title | Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title_full | Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title_fullStr | Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title_full_unstemmed | Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title_short | Improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| title_sort | improved functional assessment of osteoarthritic knee joint after chondrogenically induced cell treatment |
| url | http://psasir.upm.edu.my/id/eprint/45078/ http://psasir.upm.edu.my/id/eprint/45078/ http://psasir.upm.edu.my/id/eprint/45078/ http://psasir.upm.edu.my/id/eprint/45078/1/Improved%20functional%20assessment%20of%20osteoarthritic%20knee%20joint%20after%20chondrogenically%20induced%20cell%20treatment.pdf |