Expression and characterization of Geobacillus stearothermophilus SR74 recombinant a-Amylase in Pichia pastoris
Geobacillus stearothermophilus SR74 is a locally isolated thermophilic bacteria producing thermostable and thermoactive α-amylase. Increased production and commercialization of thermostable α-amylase strongly warrant the need of a suitable expression system. In this study, the gene encoding the ther...
| Main Authors: | Gandhi, Sivasangkary, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha, Thean, Adam Chor Leow, Oslan, Siti Nurbaya |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/44734/ http://psasir.upm.edu.my/id/eprint/44734/1/Expression%20and%20characterization%20of%20Geobacillus%20stearothermophilus%20.pdf |
Similar Items
Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115
by: Gandhi, Sivasangkary
Published: (2014)
by: Gandhi, Sivasangkary
Published: (2014)
Molecular expression of a recombinant thermostable bacterial amylase from geobacillus stearothermophilus SR74 using methanol-free meyerozyma guilliermondii strain SO yeast system
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae
by: Oslan, Siti Nurbaya, et al.
Published: (2014)
by: Oslan, Siti Nurbaya, et al.
Published: (2014)
Structure prediction of a thermostable SR74 α-Amylase from Geobacillus stearothermophilus expressed in CTG-clade yeast Meyerozyma guilliermondii strain SO
by: Lim, Si Jie, et al.
Published: (2020)
by: Lim, Si Jie, et al.
Published: (2020)
Characteristics of recombinant maltogenic amylase from Geobacillus sp. SK70
by: Sulong, Moohamad Ropaning, et al.
Published: (2017)
by: Sulong, Moohamad Ropaning, et al.
Published: (2017)
Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α-factor secretion signal in Pichia pastoris
by: Ahmad Latiffi, Amaliawati, et al.
Published: (2013)
by: Ahmad Latiffi, Amaliawati, et al.
Published: (2013)
Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris
by: Ahmad Latiffi, Amaliawati
Published: (2008)
by: Ahmad Latiffi, Amaliawati
Published: (2008)
Method for producing a recombinant thermostable geobacillus T1 lipase
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris
by: Shahidan, Noor Hidayah, et al.
Published: (2011)
by: Shahidan, Noor Hidayah, et al.
Published: (2011)
Thermodynamic evaluation and molecular dynamic simulation of thermostable lipase from ; Bacillus stearothermophilus PI, Bacillus stearothermophilus LI and Geobacillus stearothermophilus Strain TI
by: Abdul Rahman, Mohd Basyaruddin, et al.
Published: (2007)
by: Abdul Rahman, Mohd Basyaruddin, et al.
Published: (2007)
Characterisation and stabilisation of recombinant
Humicola insolens endoglucanase produced in Pichia pastoris
by: Abdul Fattah, S.S., et al.
Published: (2015)
by: Abdul Fattah, S.S., et al.
Published: (2015)
Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris
by: Sabri, Suriana
Published: (2007)
by: Sabri, Suriana
Published: (2007)
Production of thermostable L2 lipase using recombinant Pichia pastoris
by: Shahidan, Noor Hidayah
Published: (2012)
by: Shahidan, Noor Hidayah
Published: (2012)
Expression of recombinant thermostable W200R protease in Pichia pastoris
by: Mohamad Sultan, Muhamad Zarir
Published: (2011)
by: Mohamad Sultan, Muhamad Zarir
Published: (2011)
Novel geobacillus microorganism
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
Development and validation of promoter prediction system for recombinant lipase expression in Pichia pastoris
by: Gogo, Mayaki Fatima
Published: (2017)
by: Gogo, Mayaki Fatima
Published: (2017)
Purification and characterization of recombinant thermostable T1 lipase expressed from Pichia pastoris
by: Periyasamy, Nalini
Published: (2015)
by: Periyasamy, Nalini
Published: (2015)
Cloning and expression of the Glomerella cingulata cutinase recombinant gene in the yeast, Pichia pastoris
by: Lias, Norhatiah Md., et al.
Published: (2004)
by: Lias, Norhatiah Md., et al.
Published: (2004)
Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity
by: Chan, Mooi Kwai
Published: (2017)
by: Chan, Mooi Kwai
Published: (2017)
Lipase from Geobacillus Sp. Strain T1
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)
A thermoalkaliphilic lipase of Geobacillus sp. T1
by: Leow, Thean Chor, et al.
Published: (2007)
by: Leow, Thean Chor, et al.
Published: (2007)
Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris
by: Mohd Dali, Nor Soleha, et al.
Published: (2011)
by: Mohd Dali, Nor Soleha, et al.
Published: (2011)
Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.
by: Mohd Dali , Nor Soleha, et al.
Published: (2011)
by: Mohd Dali , Nor Soleha, et al.
Published: (2011)
Feeding Strategies In Recombinant Human Erythropoietin Production
By Pichia Pastoris Fermentation
In Stirred-Tank Bioreactor
by: Shahkillahwati , Mohd Ridhwan
Published: (2014)
by: Shahkillahwati , Mohd Ridhwan
Published: (2014)
Development Of High Cell Density Recombinant Pichia Pastoris Culture For Human Growth Hormone Production
by: Lee, Yoeu Chek
Published: (2008)
by: Lee, Yoeu Chek
Published: (2008)
Recombinant thermostable maltogenic amylase from Geobacillus sp. Sk70 and its variants
by: Sulong, Moohamad Ropaning
Published: (2015)
by: Sulong, Moohamad Ropaning
Published: (2015)
Characterisation of a recombinant β-xylosidase
(xylA) from Aspergillus oryzae expressed in Pichia
pastoris
by: Kirikyali, Narin, et al.
Published: (2014)
by: Kirikyali, Narin, et al.
Published: (2014)
Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris
by: de la Cruz, Silvia, et al.
Published: (2016)
by: de la Cruz, Silvia, et al.
Published: (2016)
Cloning of Aspergillus Niger BglA and expression of recombinant β-glucosidase in methylotrophic yeast Pichia Pastoris
by: Kamaruddin, Shazilah, et al.
Published: (2008)
by: Kamaruddin, Shazilah, et al.
Published: (2008)
Use of a newly isolated yeast in the expression of recombinant proteins
by: Salleh, Abu Bakar, et al.
Published: (2012)
by: Salleh, Abu Bakar, et al.
Published: (2012)
High level expression of thermostable lipase from Geobacillus sp. strain T1
by: Leow, Thean Chor, et al.
Published: (2004)
by: Leow, Thean Chor, et al.
Published: (2004)
Ion interaction and hydrogen bonds as main features of protein thermostability in mutated T1 recombinant lipase originated from Geobacillus zalihae
by: Ishak, Siti Nor Hasmah, et al.
Published: (2020)
by: Ishak, Siti Nor Hasmah, et al.
Published: (2020)
Transformation of Chitinase in Yeast Pichia Pastoris GS115
by: Rasyidah, binti Ramlee
Published: (2015)
by: Rasyidah, binti Ramlee
Published: (2015)
A newly isolated yeast as an expression host for recombinant lipase
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris
by: Amatul Samahah Md Ali,, et al.
Published: (2015)
by: Amatul Samahah Md Ali,, et al.
Published: (2015)
Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris
by: Tam, Yew Joon, et al.
Published: (2016)
by: Tam, Yew Joon, et al.
Published: (2016)
Production of long helical capsid of Nipah virus by Pichia pastoris.
by: Joseph, Narcisse M. S., et al.
Published: (2011)
by: Joseph, Narcisse M. S., et al.
Published: (2011)
Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
by: Nguyen, Khanh Hoang Viet, et al.
Published: (2022)
by: Nguyen, Khanh Hoang Viet, et al.
Published: (2022)
Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris
by: Md Tab M.,, et al.
Published: (2017)
by: Md Tab M.,, et al.
Published: (2017)
Production and Characterisation of Thermostable Protease from Bacillus Stearothermophilus Strain F1
by: Raja Abd. Rahman, Raja Noor Zaliha
Published: (1994)
by: Raja Abd. Rahman, Raja Noor Zaliha
Published: (1994)
Similar Items
-
Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115
by: Gandhi, Sivasangkary
Published: (2014) -
Molecular expression of a recombinant thermostable bacterial amylase from geobacillus stearothermophilus SR74 using methanol-free meyerozyma guilliermondii strain SO yeast system
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020) -
Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae
by: Oslan, Siti Nurbaya, et al.
Published: (2014) -
Structure prediction of a thermostable SR74 α-Amylase from Geobacillus stearothermophilus expressed in CTG-clade yeast Meyerozyma guilliermondii strain SO
by: Lim, Si Jie, et al.
Published: (2020) -
Characteristics of recombinant maltogenic amylase from Geobacillus sp. SK70
by: Sulong, Moohamad Ropaning, et al.
Published: (2017)