Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections
Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicem...
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| Format: | Article |
| Language: | English |
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Veterinary World
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/44076/ http://psasir.upm.edu.my/id/eprint/44076/1/Clinico-pathology%2C%20hematology%2C%20and%20biochemistry%20responses%20toward%20Pasteurella%20multocida%20type%20B%202%20via%20oral%20and%20subcutaneous%20route%20of%20infections.pdf |
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| author | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abdul Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Omar, Abdul Rahman Abu Bakar, Md Zuki Norsidin, Mohd Jefri |
| author_facet | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abdul Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Omar, Abdul Rahman Abu Bakar, Md Zuki Norsidin, Mohd Jefri |
| author_sort | Lim, Eric Teik Chung |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 10(12) colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastro-intestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future. |
| first_indexed | 2025-11-15T10:05:33Z |
| format | Article |
| id | upm-44076 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:05:33Z |
| publishDate | 2015 |
| publisher | Veterinary World |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-440762022-03-10T08:49:54Z http://psasir.upm.edu.my/id/eprint/44076/ Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abdul Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Omar, Abdul Rahman Abu Bakar, Md Zuki Norsidin, Mohd Jefri Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 10(12) colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastro-intestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future. Veterinary World 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/44076/1/Clinico-pathology%2C%20hematology%2C%20and%20biochemistry%20responses%20toward%20Pasteurella%20multocida%20type%20B%202%20via%20oral%20and%20subcutaneous%20route%20of%20infections.pdf Lim, Eric Teik Chung and Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Marza, Ali Dhiaa and Ibrahim, Hayder Hamzah and Saad, Mohd Zamri and Haron, Abdul Wahid and Saharee, Abdul Aziz and Mohd Lila, Mohd Azmi and Omar, Abdul Rahman and Abu Bakar, Md Zuki and Norsidin, Mohd Jefri (2015) Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections. Veterinary World, 8 (6). pp. 783-792. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825283/ 10.14202/vetworld.2015.783-792 |
| spellingShingle | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Adamu, Lawan Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abdul Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Omar, Abdul Rahman Abu Bakar, Md Zuki Norsidin, Mohd Jefri Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title_full | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title_fullStr | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title_full_unstemmed | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title_short | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida type B: 2 via oral and subcutaneous route of infections |
| title_sort | clinico-pathology, hematology, and biochemistry responses toward pasteurella multocida type b: 2 via oral and subcutaneous route of infections |
| url | http://psasir.upm.edu.my/id/eprint/44076/ http://psasir.upm.edu.my/id/eprint/44076/ http://psasir.upm.edu.my/id/eprint/44076/ http://psasir.upm.edu.my/id/eprint/44076/1/Clinico-pathology%2C%20hematology%2C%20and%20biochemistry%20responses%20toward%20Pasteurella%20multocida%20type%20B%202%20via%20oral%20and%20subcutaneous%20route%20of%20infections.pdf |