Association of azoospermia factor region deletions in infertile male subjects among Malaysians

Azoospermia factor region (AZF) deletions (AZFa, AZFb, AZFc and AZFd) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZFa, AZFb, AZFc and AZFd del...

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Main Authors: Hussein, A. A., Vasudevan, R., Ismail, Patimah, Prashant, N., Nora, F. A.
Format: Article
Language:English
Published: Wiley 2015
Online Access:http://psasir.upm.edu.my/id/eprint/43879/
http://psasir.upm.edu.my/id/eprint/43879/1/Association%20of%20azoospermia%20factor%20region%20deletions%20in%20infertile%20male%20subjects%20among%20Malaysians.pdf
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author Hussein, A. A.
Vasudevan, R.
Ismail, Patimah
Prashant, N.
Nora, F. A.
author_facet Hussein, A. A.
Vasudevan, R.
Ismail, Patimah
Prashant, N.
Nora, F. A.
author_sort Hussein, A. A.
building UPM Institutional Repository
collection Online Access
description Azoospermia factor region (AZF) deletions (AZFa, AZFb, AZFc and AZFd) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZFa, AZFb, AZFc and AZFd deletions were determined using conventional polymerase chain reaction (PCR) as well as real-time PCR-high resolution melting analysis-based methods. The results of this study showed that, three of 54 cases (5.55%) had AZF (a, b and c) deletions (two had AZFc and one had AZFa deletions). Four cases were found to have AZFd deletions (7.4%) with two of them being associated with AZFc deletions (P = 0.028). The frequency of AZF (a, b and c) deletions in Malaysian infertile male subjects was found to be comparable with other populations. AZFd deletions were found to be significant (P < 0.05) in male infertility and it may be associated with other types of AZF deletions.
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spelling upm-438792016-10-27T01:26:07Z http://psasir.upm.edu.my/id/eprint/43879/ Association of azoospermia factor region deletions in infertile male subjects among Malaysians Hussein, A. A. Vasudevan, R. Ismail, Patimah Prashant, N. Nora, F. A. Azoospermia factor region (AZF) deletions (AZFa, AZFb, AZFc and AZFd) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZFa, AZFb, AZFc and AZFd deletions were determined using conventional polymerase chain reaction (PCR) as well as real-time PCR-high resolution melting analysis-based methods. The results of this study showed that, three of 54 cases (5.55%) had AZF (a, b and c) deletions (two had AZFc and one had AZFa deletions). Four cases were found to have AZFd deletions (7.4%) with two of them being associated with AZFc deletions (P = 0.028). The frequency of AZF (a, b and c) deletions in Malaysian infertile male subjects was found to be comparable with other populations. AZFd deletions were found to be significant (P < 0.05) in male infertility and it may be associated with other types of AZF deletions. Wiley 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43879/1/Association%20of%20azoospermia%20factor%20region%20deletions%20in%20infertile%20male%20subjects%20among%20Malaysians.pdf Hussein, A. A. and Vasudevan, R. and Ismail, Patimah and Prashant, N. and Nora, F. A. (2015) Association of azoospermia factor region deletions in infertile male subjects among Malaysians. Andrologia, 47 (1). pp. 168-177. ISSN 0303-4569; ESSN: 1439-0272 10.1111/and.12240
spellingShingle Hussein, A. A.
Vasudevan, R.
Ismail, Patimah
Prashant, N.
Nora, F. A.
Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title_full Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title_fullStr Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title_full_unstemmed Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title_short Association of azoospermia factor region deletions in infertile male subjects among Malaysians
title_sort association of azoospermia factor region deletions in infertile male subjects among malaysians
url http://psasir.upm.edu.my/id/eprint/43879/
http://psasir.upm.edu.my/id/eprint/43879/
http://psasir.upm.edu.my/id/eprint/43879/1/Association%20of%20azoospermia%20factor%20region%20deletions%20in%20infertile%20male%20subjects%20among%20Malaysians.pdf