Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells
The increase in endothelial permeability often promotes edema formation in various pathological conditions. Tumor necrosis factor-alpha (TNF-α), a pro-atherogenic cytokine, impairs endothelial barrier function and causes endothelial dysfunction in early stage of atherosclerosis. Asiaticoside, one of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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John Wiley & Sons
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43838/ http://psasir.upm.edu.my/id/eprint/43838/1/Asiaticoside%20inhibits%20TNF-%CE%B1-induced%20endothelial%20hyperpermeability%20of%20human%20aortic%20endothelial%20cells.pdf |
| _version_ | 1848850336766230528 |
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| author | Lai, Yen Fong Chin, Theng Ng Zakaria, Zainul Amiruddin Baharuldin, Mohamad Taufik Hidayat Abd. Kadir, Arifah Abdullah, Muhammad Nazrul Hakim Ahmad, Zuraini |
| author_facet | Lai, Yen Fong Chin, Theng Ng Zakaria, Zainul Amiruddin Baharuldin, Mohamad Taufik Hidayat Abd. Kadir, Arifah Abdullah, Muhammad Nazrul Hakim Ahmad, Zuraini |
| author_sort | Lai, Yen Fong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The increase in endothelial permeability often promotes edema formation in various pathological conditions. Tumor necrosis factor-alpha (TNF-α), a pro-atherogenic cytokine, impairs endothelial barrier function and causes endothelial dysfunction in early stage of atherosclerosis. Asiaticoside, one of the triterpenoids derived from Centella asiatica, is known to possess antiinflammatory activity. In order to examine the role of asiaticoside in preserving the endothelial barrier, we assessed its effects on endothelial hyperpermeability and disruption of actin filaments evoked by TNF-α in human aortic endothelial cells (HAEC). TNF-α caused an increase in endothelial permeability to fluorescein isothiocyanate (FITC)-dextran. Asiaticoside pretreatment significantly suppressed TNF-α-induced increased permeability. Asiaticoside also prevented TNF-α-induced actin redistribution by suppressing stress fiber formation. However, the increased F to G actin ratio stimulated by TNF-α was not changed by asiaticoside. Cytochalasin D, an actin depolymerizing agent, was used to correlate the anti-hyperpermeability effect of asiaticoside with actin cytoskeleton. Surprisingly, asiaticoside failed to prevent cytochalasin D-induced increased permeability. These results suggest that asiaticoside protects against the disruption of endothelial barrier and actin rearrangement triggered by TNF-α without a significant change in total actin pool. However, asiaticoside seems to work by other mechanisms to maintain the integrity of endothelial barrier rather than stabilizing the F-actin organization. |
| first_indexed | 2025-11-15T10:04:41Z |
| format | Article |
| id | upm-43838 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:04:41Z |
| publishDate | 2015 |
| publisher | John Wiley & Sons |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-438382016-09-21T08:04:43Z http://psasir.upm.edu.my/id/eprint/43838/ Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells Lai, Yen Fong Chin, Theng Ng Zakaria, Zainul Amiruddin Baharuldin, Mohamad Taufik Hidayat Abd. Kadir, Arifah Abdullah, Muhammad Nazrul Hakim Ahmad, Zuraini The increase in endothelial permeability often promotes edema formation in various pathological conditions. Tumor necrosis factor-alpha (TNF-α), a pro-atherogenic cytokine, impairs endothelial barrier function and causes endothelial dysfunction in early stage of atherosclerosis. Asiaticoside, one of the triterpenoids derived from Centella asiatica, is known to possess antiinflammatory activity. In order to examine the role of asiaticoside in preserving the endothelial barrier, we assessed its effects on endothelial hyperpermeability and disruption of actin filaments evoked by TNF-α in human aortic endothelial cells (HAEC). TNF-α caused an increase in endothelial permeability to fluorescein isothiocyanate (FITC)-dextran. Asiaticoside pretreatment significantly suppressed TNF-α-induced increased permeability. Asiaticoside also prevented TNF-α-induced actin redistribution by suppressing stress fiber formation. However, the increased F to G actin ratio stimulated by TNF-α was not changed by asiaticoside. Cytochalasin D, an actin depolymerizing agent, was used to correlate the anti-hyperpermeability effect of asiaticoside with actin cytoskeleton. Surprisingly, asiaticoside failed to prevent cytochalasin D-induced increased permeability. These results suggest that asiaticoside protects against the disruption of endothelial barrier and actin rearrangement triggered by TNF-α without a significant change in total actin pool. However, asiaticoside seems to work by other mechanisms to maintain the integrity of endothelial barrier rather than stabilizing the F-actin organization. John Wiley & Sons 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43838/1/Asiaticoside%20inhibits%20TNF-%CE%B1-induced%20endothelial%20hyperpermeability%20of%20human%20aortic%20endothelial%20cells.pdf Lai, Yen Fong and Chin, Theng Ng and Zakaria, Zainul Amiruddin and Baharuldin, Mohamad Taufik Hidayat and Abd. Kadir, Arifah and Abdullah, Muhammad Nazrul Hakim and Ahmad, Zuraini (2015) Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells. Phytotherapy Research, 29 (10). pp. 1501-1508. ISSN 0951-418X; ESSN: 1099-1573 http://onlinelibrary.wiley.com/doi/10.1002/ptr.5404/pdf 10.1002/ptr.5404 |
| spellingShingle | Lai, Yen Fong Chin, Theng Ng Zakaria, Zainul Amiruddin Baharuldin, Mohamad Taufik Hidayat Abd. Kadir, Arifah Abdullah, Muhammad Nazrul Hakim Ahmad, Zuraini Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title | Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title_full | Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title_fullStr | Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title_full_unstemmed | Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title_short | Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| title_sort | asiaticoside inhibits tnf-alpha-induced endothelial hyperpermeability of human aortic endothelial cells |
| url | http://psasir.upm.edu.my/id/eprint/43838/ http://psasir.upm.edu.my/id/eprint/43838/ http://psasir.upm.edu.my/id/eprint/43838/ http://psasir.upm.edu.my/id/eprint/43838/1/Asiaticoside%20inhibits%20TNF-%CE%B1-induced%20endothelial%20hyperpermeability%20of%20human%20aortic%20endothelial%20cells.pdf |