Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach
Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43810/ http://psasir.upm.edu.my/id/eprint/43810/1/Apoptosis%20Induction%20in%20Human%20Leukemia%20Cell%20Lines%20by%20Gold%20Nanoparticles%20Synthesized%20Using%20the%20Green%20Biosynthetic%20Approach.pdf |
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| author | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Rasedee, Abdullah Swee, Keong Yeap Chartrand, Max Stanley Azizi, Susan Mohd Tahir, Paridah |
| author_facet | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Rasedee, Abdullah Swee, Keong Yeap Chartrand, Max Stanley Azizi, Susan Mohd Tahir, Paridah |
| author_sort | Namvar, Farideh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. The in vitro anticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50 value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furthermore, apoptosis induction was confirmed by Hoechst 33342, annexin V staining, and caspase-3/-9 activity tests. The cell cycle analysis exhibited a significant increase in the accumulation of S-GNPs treated cells at the sub-G1 phase, demonstrating the induction of apoptosis by S-GNPs. The nature of the inhibition of cancer cell growth by S-GNPs could open the way for further research in the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer. |
| first_indexed | 2025-11-15T10:04:33Z |
| format | Article |
| id | upm-43810 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:04:33Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-438102016-09-21T04:31:24Z http://psasir.upm.edu.my/id/eprint/43810/ Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Rasedee, Abdullah Swee, Keong Yeap Chartrand, Max Stanley Azizi, Susan Mohd Tahir, Paridah Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. The in vitro anticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50 value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furthermore, apoptosis induction was confirmed by Hoechst 33342, annexin V staining, and caspase-3/-9 activity tests. The cell cycle analysis exhibited a significant increase in the accumulation of S-GNPs treated cells at the sub-G1 phase, demonstrating the induction of apoptosis by S-GNPs. The nature of the inhibition of cancer cell growth by S-GNPs could open the way for further research in the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer. Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43810/1/Apoptosis%20Induction%20in%20Human%20Leukemia%20Cell%20Lines%20by%20Gold%20Nanoparticles%20Synthesized%20Using%20the%20Green%20Biosynthetic%20Approach.pdf Namvar, Farideh and Rahman, Heshu Sulaiman and Mohamad, Rosfarizan and Rasedee, Abdullah and Swee, Keong Yeap and Chartrand, Max Stanley and Azizi, Susan and Mohd Tahir, Paridah (2015) Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach. Journal of Nanomaterials, 2015. art. no. 642621. pp. 1-10. ISSN 1687-4110; ESSN: 1687-4129 http://www.hindawi.com/journals/jnm/2015/642621/ 10.1155/2015/642621 |
| spellingShingle | Namvar, Farideh Rahman, Heshu Sulaiman Mohamad, Rosfarizan Rasedee, Abdullah Swee, Keong Yeap Chartrand, Max Stanley Azizi, Susan Mohd Tahir, Paridah Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title | Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title_full | Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title_fullStr | Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title_full_unstemmed | Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title_short | Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| title_sort | apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach |
| url | http://psasir.upm.edu.my/id/eprint/43810/ http://psasir.upm.edu.my/id/eprint/43810/ http://psasir.upm.edu.my/id/eprint/43810/ http://psasir.upm.edu.my/id/eprint/43810/1/Apoptosis%20Induction%20in%20Human%20Leukemia%20Cell%20Lines%20by%20Gold%20Nanoparticles%20Synthesized%20Using%20the%20Green%20Biosynthetic%20Approach.pdf |