Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach
Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43810/ http://psasir.upm.edu.my/id/eprint/43810/1/Apoptosis%20Induction%20in%20Human%20Leukemia%20Cell%20Lines%20by%20Gold%20Nanoparticles%20Synthesized%20Using%20the%20Green%20Biosynthetic%20Approach.pdf |
| Summary: | Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. The in vitro anticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50 value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furthermore, apoptosis induction was confirmed by Hoechst 33342, annexin V staining, and caspase-3/-9 activity tests. The cell cycle analysis exhibited a significant increase in the accumulation of S-GNPs treated cells at the sub-G1 phase, demonstrating the induction of apoptosis by S-GNPs. The nature of the inhibition of cancer cell growth by S-GNPs could open the way for further research in the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer. |
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