Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate
Protein extracted from oil palm kernel was hydrolyzed using pepsin-pancreatin (OPKH) and subsequently used to identify the potential antioxidative peptides. Then, the OPKH was purified sequentially by ultrafiltration, reversed-phase and semi-preparative HPLC. Antioxidant capacities (ACs) of the puri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43801/ http://psasir.upm.edu.my/id/eprint/43801/1/Antioxidant%20peptides%20purified%20and%20identified%20from%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20.pdf |
| _version_ | 1848850326424125440 |
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| author | Sui, Kiat Chang Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat |
| author_facet | Sui, Kiat Chang Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat |
| author_sort | Sui, Kiat Chang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Protein extracted from oil palm kernel was hydrolyzed using pepsin-pancreatin (OPKH) and subsequently used to identify the potential antioxidative peptides. Then, the OPKH was purified sequentially by ultrafiltration, reversed-phase and semi-preparative HPLC. Antioxidant capacities (ACs) of the purified peptides from OPKH were evaluated using ferric reducing antioxidant power (FRAP), β-carotene-linoleate bleaching (BCB) assay and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity assay. Amino acid compositions of the purified peptide fractions were also determined. The amino acid sequence of the antioxidative peptide was identified by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS) using de novo sequencing. There were nine fractions purified and collected where F6 demonstrated the highest AC. Three antioxidative peptides, Val-Val-Gly-Gly-Asp-Gly-Asp-Val (VVGGDGDV), Val-Pro-Val-Thr-Ser-Thr (VPVTST) and Leu-Thr-Thr-Leu-Asp-Ser-Glu (LTTLDSE) were identified in fraction F6. These peptides did not show any similarity with other antioxidant peptides listed in BLAST database of NCBI. These peptides may be useful ingredients in food and pharmaceutical applications. |
| first_indexed | 2025-11-15T10:04:31Z |
| format | Article |
| id | upm-43801 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:04:31Z |
| publishDate | 2015 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-438012018-04-09T07:47:15Z http://psasir.upm.edu.my/id/eprint/43801/ Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate Sui, Kiat Chang Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat Protein extracted from oil palm kernel was hydrolyzed using pepsin-pancreatin (OPKH) and subsequently used to identify the potential antioxidative peptides. Then, the OPKH was purified sequentially by ultrafiltration, reversed-phase and semi-preparative HPLC. Antioxidant capacities (ACs) of the purified peptides from OPKH were evaluated using ferric reducing antioxidant power (FRAP), β-carotene-linoleate bleaching (BCB) assay and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity assay. Amino acid compositions of the purified peptide fractions were also determined. The amino acid sequence of the antioxidative peptide was identified by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS) using de novo sequencing. There were nine fractions purified and collected where F6 demonstrated the highest AC. Three antioxidative peptides, Val-Val-Gly-Gly-Asp-Gly-Asp-Val (VVGGDGDV), Val-Pro-Val-Thr-Ser-Thr (VPVTST) and Leu-Thr-Thr-Leu-Asp-Ser-Glu (LTTLDSE) were identified in fraction F6. These peptides did not show any similarity with other antioxidant peptides listed in BLAST database of NCBI. These peptides may be useful ingredients in food and pharmaceutical applications. Elsevier BV 2015-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43801/1/Antioxidant%20peptides%20purified%20and%20identified%20from%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20.pdf Sui, Kiat Chang and Ismail, Amin and Yanagita, Teruyoshi and Mohd Esa, Norhaizan and Baharuldin, Mohamad Taufik Hidayat (2015) Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate. Journal of Functional Foods, 14. pp. 63-75. ISSN 1756-4646; ESSN: 2214-9414 10.1016/j.jff.2015.01.011 |
| spellingShingle | Sui, Kiat Chang Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title | Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title_full | Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title_fullStr | Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title_full_unstemmed | Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title_short | Antioxidant peptides purified and identified from the oil palm (Elaeis guineensis Jacq.) kernel protein hydrolysate |
| title_sort | antioxidant peptides purified and identified from the oil palm (elaeis guineensis jacq.) kernel protein hydrolysate |
| url | http://psasir.upm.edu.my/id/eprint/43801/ http://psasir.upm.edu.my/id/eprint/43801/ http://psasir.upm.edu.my/id/eprint/43801/1/Antioxidant%20peptides%20purified%20and%20identified%20from%20the%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20.pdf |