Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract

The aim of this study is to determine the antilipase and antioxidant activity of Phyllanthus niruri methanolic extract. The inhibitory activity against porcine pancreatic lipase (PPL) was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. The crude extract e...

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Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Alias, Norsyuhada, Leow, Adam Thean Chor, Mohamad Ali, Mohd Shukuri, Ahmad Tajudin, Asilah, Salleh, Abu Bakar
Format: Article
Language:English
Published: American-Eurasian Network for Scientific Information 2015
Online Access:http://psasir.upm.edu.my/id/eprint/43793/
http://psasir.upm.edu.my/id/eprint/43793/1/Antilipase%20and%20antioxidant%20activity%20of%20Phyllanthus%20niruri%20ethanolic%20extract%20.pdf
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author Raja Abdul Rahman, Raja Noor Zaliha
Alias, Norsyuhada
Leow, Adam Thean Chor
Mohamad Ali, Mohd Shukuri
Ahmad Tajudin, Asilah
Salleh, Abu Bakar
author_facet Raja Abdul Rahman, Raja Noor Zaliha
Alias, Norsyuhada
Leow, Adam Thean Chor
Mohamad Ali, Mohd Shukuri
Ahmad Tajudin, Asilah
Salleh, Abu Bakar
author_sort Raja Abdul Rahman, Raja Noor Zaliha
building UPM Institutional Repository
collection Online Access
description The aim of this study is to determine the antilipase and antioxidant activity of Phyllanthus niruri methanolic extract. The inhibitory activity against porcine pancreatic lipase (PPL) was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. The crude extract exhibited strong lipase inhibitory activity with an IC50 value of 27. 65 µg/ mL. The inhibition mode study disclosed that P. niruri could act as noncompetitive inhibitor. Antioxidant activity of P. niruri extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. P. niruri showed high antioxidant activity with an EC50 value of 7.471 µg/mL. The results suggested that P. niruri has shown potential as a source of natural antilipase and antioxidant.
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institution Universiti Putra Malaysia
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language English
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publishDate 2015
publisher American-Eurasian Network for Scientific Information
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spelling upm-437932018-09-24T00:47:10Z http://psasir.upm.edu.my/id/eprint/43793/ Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract Raja Abdul Rahman, Raja Noor Zaliha Alias, Norsyuhada Leow, Adam Thean Chor Mohamad Ali, Mohd Shukuri Ahmad Tajudin, Asilah Salleh, Abu Bakar The aim of this study is to determine the antilipase and antioxidant activity of Phyllanthus niruri methanolic extract. The inhibitory activity against porcine pancreatic lipase (PPL) was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. The crude extract exhibited strong lipase inhibitory activity with an IC50 value of 27. 65 µg/ mL. The inhibition mode study disclosed that P. niruri could act as noncompetitive inhibitor. Antioxidant activity of P. niruri extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. P. niruri showed high antioxidant activity with an EC50 value of 7.471 µg/mL. The results suggested that P. niruri has shown potential as a source of natural antilipase and antioxidant. American-Eurasian Network for Scientific Information 2015-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43793/1/Antilipase%20and%20antioxidant%20activity%20of%20Phyllanthus%20niruri%20ethanolic%20extract%20.pdf Raja Abdul Rahman, Raja Noor Zaliha and Alias, Norsyuhada and Leow, Adam Thean Chor and Mohamad Ali, Mohd Shukuri and Ahmad Tajudin, Asilah and Salleh, Abu Bakar (2015) Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract. Australian Journal of Basic and Applied Sciences, 9 (7). pp. 133-136. ISSN 1991-8178 http://www.ajbasweb.com/old/ajbas_April_2015.html
spellingShingle Raja Abdul Rahman, Raja Noor Zaliha
Alias, Norsyuhada
Leow, Adam Thean Chor
Mohamad Ali, Mohd Shukuri
Ahmad Tajudin, Asilah
Salleh, Abu Bakar
Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title_full Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title_fullStr Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title_full_unstemmed Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title_short Antilipase and antioxidant activity of Phyllanthus niruri ethanolic extract
title_sort antilipase and antioxidant activity of phyllanthus niruri ethanolic extract
url http://psasir.upm.edu.my/id/eprint/43793/
http://psasir.upm.edu.my/id/eprint/43793/
http://psasir.upm.edu.my/id/eprint/43793/1/Antilipase%20and%20antioxidant%20activity%20of%20Phyllanthus%20niruri%20ethanolic%20extract%20.pdf