Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes
Objective: To explore whether its antiplasmodium effect of andrographolide is attributed to its plausible effect on the plasma membrane of both Plasmodium falciparum infected and non-infected RBCs. Methods: Anti-plasmodium effect of andrographolide against Plasmodium falciparum strains was scre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43771/ http://psasir.upm.edu.my/id/eprint/43771/1/Andrographolide%20effect%20on%20both%20Plasmodium%20falciparum%20infected%20and%20non%20infected%20RBCs%20membranes.pdf |
| _version_ | 1848850318372110336 |
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| author | Ibraheem, Zaid Osamah Abd. Majid, Roslaini Md Noor, Sabariah Mohd Sidek, Hasidah Al-Zihiry, Khalid Jameel Kadhim Yam, Mun Fei Basir, Rusliza |
| author_facet | Ibraheem, Zaid Osamah Abd. Majid, Roslaini Md Noor, Sabariah Mohd Sidek, Hasidah Al-Zihiry, Khalid Jameel Kadhim Yam, Mun Fei Basir, Rusliza |
| author_sort | Ibraheem, Zaid Osamah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective:
To explore whether its antiplasmodium effect of andrographolide is attributed to its plausible effect on the plasma membrane of both Plasmodium falciparum infected and non-infected RBCs.
Methods:
Anti-plasmodium effect of andrographolide against Plasmodium falciparum strains was screened using the conventional malaria drug sensitivity assay. The drug was incubated with uninfected RBCs to monitor its effect on their morphology, integrity and osmotic fragility. It was incubated with the plasmodium infected RBCs to monitor its effect on the parasite induced permeation pathways. Its effect on the potential of merozoites to invade new RBCs was tested using merozoite invasion assay.
Results:
It showed that at andrographolide was innocuous to RBCs at concentrations approach its therapeutic level against plasmodia. Nevertheless, this inertness was dwindled at higher concentrations.
Conclusions:
In spite of its success to inhibit plasmodium induced permeation pathway and the potential of merozoites to invade new RBCs, its anti-plasmodium effect can't be attributed to these functions as they were attained at concentrations higher than what is required to eradicate the parasite. Consequently, other mechanisms may be associated with its claimed actions. |
| first_indexed | 2025-11-15T10:04:23Z |
| format | Article |
| id | upm-43771 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:04:23Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-437712017-10-27T09:11:16Z http://psasir.upm.edu.my/id/eprint/43771/ Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes Ibraheem, Zaid Osamah Abd. Majid, Roslaini Md Noor, Sabariah Mohd Sidek, Hasidah Al-Zihiry, Khalid Jameel Kadhim Yam, Mun Fei Basir, Rusliza Objective: To explore whether its antiplasmodium effect of andrographolide is attributed to its plausible effect on the plasma membrane of both Plasmodium falciparum infected and non-infected RBCs. Methods: Anti-plasmodium effect of andrographolide against Plasmodium falciparum strains was screened using the conventional malaria drug sensitivity assay. The drug was incubated with uninfected RBCs to monitor its effect on their morphology, integrity and osmotic fragility. It was incubated with the plasmodium infected RBCs to monitor its effect on the parasite induced permeation pathways. Its effect on the potential of merozoites to invade new RBCs was tested using merozoite invasion assay. Results: It showed that at andrographolide was innocuous to RBCs at concentrations approach its therapeutic level against plasmodia. Nevertheless, this inertness was dwindled at higher concentrations. Conclusions: In spite of its success to inhibit plasmodium induced permeation pathway and the potential of merozoites to invade new RBCs, its anti-plasmodium effect can't be attributed to these functions as they were attained at concentrations higher than what is required to eradicate the parasite. Consequently, other mechanisms may be associated with its claimed actions. Elsevier 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43771/1/Andrographolide%20effect%20on%20both%20Plasmodium%20falciparum%20infected%20and%20non%20infected%20RBCs%20membranes.pdf Ibraheem, Zaid Osamah and Abd. Majid, Roslaini and Md Noor, Sabariah and Mohd Sidek, Hasidah and Al-Zihiry, Khalid Jameel Kadhim and Yam, Mun Fei and Basir, Rusliza (2015) Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes. Asian Pacific Journal of Tropical Medicine, 8 (7). pp. 507-512. ISSN 1995-7645 10.1016/j.apjtm.2015.06.007 |
| spellingShingle | Ibraheem, Zaid Osamah Abd. Majid, Roslaini Md Noor, Sabariah Mohd Sidek, Hasidah Al-Zihiry, Khalid Jameel Kadhim Yam, Mun Fei Basir, Rusliza Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title | Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title_full | Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title_fullStr | Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title_full_unstemmed | Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title_short | Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes |
| title_sort | andrographolide effect on both plasmodium falciparum infected and non infected rbcs membranes |
| url | http://psasir.upm.edu.my/id/eprint/43771/ http://psasir.upm.edu.my/id/eprint/43771/ http://psasir.upm.edu.my/id/eprint/43771/1/Andrographolide%20effect%20on%20both%20Plasmodium%20falciparum%20infected%20and%20non%20infected%20RBCs%20membranes.pdf |