α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions
Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of α-mangostin, a major xanthone...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43690/ http://psasir.upm.edu.my/id/eprint/43690/1/123.pdf |
| _version_ | 1848850296315314176 |
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| author | Taher, Muhammad Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Deny Susanti, Jauhari Arief Ichwan, Solachuddin Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin |
| author_facet | Taher, Muhammad Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Deny Susanti, Jauhari Arief Ichwan, Solachuddin Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin |
| author_sort | Taher, Muhammad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of α-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPARγ, GLUT4, and leptin expressions. α-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 μM of α-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake ( P < 0.05 ) with highest activity found at 25 μM. In addition, α-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 μM of α-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPARγ gene expression. Induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that α-mangostin might be possible candidate for the effective management of obesity in future. |
| first_indexed | 2025-11-15T10:04:02Z |
| format | Article |
| id | upm-43690 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:04:02Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-436902016-08-08T09:24:52Z http://psasir.upm.edu.my/id/eprint/43690/ α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions Taher, Muhammad Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Deny Susanti, Jauhari Arief Ichwan, Solachuddin Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of α-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPARγ, GLUT4, and leptin expressions. α-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 μM of α-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake ( P < 0.05 ) with highest activity found at 25 μM. In addition, α-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 μM of α-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPARγ gene expression. Induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that α-mangostin might be possible candidate for the effective management of obesity in future. Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43690/1/123.pdf Taher, Muhammad and Mohamed Amiroudine, Mohamed Zaffar Ali and Tengku Zakaria, Tengku Muhamad Faris Syafiq and Deny Susanti, and Jauhari Arief Ichwan, Solachuddin and Kaderi, Mohd Arifin and Ahmed, Qamar Uddin and Zakaria, Zainul Amiruddin (2015) α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions. Evidence-Based Complementary and Alternative Medicine, 2015. art. no. 74023. pp. 1-9. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2015/740238/ 10.1155/2015/740238 |
| spellingShingle | Taher, Muhammad Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Deny Susanti, Jauhari Arief Ichwan, Solachuddin Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title_full | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title_fullStr | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title_full_unstemmed | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title_short | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPARgamma, GLUT4, and leptin expressions |
| title_sort | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3t3-l1 cells via ppargamma, glut4, and leptin expressions |
| url | http://psasir.upm.edu.my/id/eprint/43690/ http://psasir.upm.edu.my/id/eprint/43690/ http://psasir.upm.edu.my/id/eprint/43690/ http://psasir.upm.edu.my/id/eprint/43690/1/123.pdf |