A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection
In this study, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. Firstly, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-Aminopropyl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IEEE
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43536/ http://psasir.upm.edu.my/id/eprint/43536/1/abstract00.pdf |
| _version_ | 1848850254912290816 |
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| author | Abd Rashid, Jahwarhar Izuan Yusof, Nor Azah Abdullah, Jaafar Hashim, Uda Hajian, Reza |
| author_facet | Abd Rashid, Jahwarhar Izuan Yusof, Nor Azah Abdullah, Jaafar Hashim, Uda Hajian, Reza |
| author_sort | Abd Rashid, Jahwarhar Izuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. Firstly, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-Aminopropyltriethoxysilane (APTES, 0.5 %) onto bare SPGE. Secondly, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid (DTPA) through a self-assembly monolayer (SAM) technique. The electrochemical response of methylene blue (MB) as a redox indicator towards synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of 1.0 × 10-11-1.0 × 10-7 M (R= 0.98) with the detection limit of 1.63× 10-12 M. |
| first_indexed | 2025-11-15T10:03:22Z |
| format | Article |
| id | upm-43536 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:03:22Z |
| publishDate | 2015 |
| publisher | IEEE |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-435362016-06-29T03:15:31Z http://psasir.upm.edu.my/id/eprint/43536/ A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection Abd Rashid, Jahwarhar Izuan Yusof, Nor Azah Abdullah, Jaafar Hashim, Uda Hajian, Reza In this study, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. Firstly, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-Aminopropyltriethoxysilane (APTES, 0.5 %) onto bare SPGE. Secondly, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid (DTPA) through a self-assembly monolayer (SAM) technique. The electrochemical response of methylene blue (MB) as a redox indicator towards synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of 1.0 × 10-11-1.0 × 10-7 M (R= 0.98) with the detection limit of 1.63× 10-12 M. IEEE 2015-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43536/1/abstract00.pdf Abd Rashid, Jahwarhar Izuan and Yusof, Nor Azah and Abdullah, Jaafar and Hashim, Uda and Hajian, Reza (2015) A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection. IEEE Sensors Journal, 15 (8). pp. 4420-4427. ISSN 1530-437X 10.1109/JSEN.2015.2417911 |
| spellingShingle | Abd Rashid, Jahwarhar Izuan Yusof, Nor Azah Abdullah, Jaafar Hashim, Uda Hajian, Reza A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title | A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title_full | A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title_fullStr | A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title_full_unstemmed | A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title_short | A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection |
| title_sort | novel disposable biosensor based on sinws/aunps modified-screen printed electrode for dengue virus dna oligomer detection |
| url | http://psasir.upm.edu.my/id/eprint/43536/ http://psasir.upm.edu.my/id/eprint/43536/ http://psasir.upm.edu.my/id/eprint/43536/1/abstract00.pdf |