A newly isolated yeast as an expression host for recombinant lipase
Pichia guilliermondii strain SO isolated from spoiled orange was developed for use as an alternative expression host by using Pichia pastoris as the model of the experiment. This is the first study to report on the capability of P. guilliermondii SO as a host to express thermostable T1 lipase from G...
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| Format: | Article |
| Language: | English |
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University of Wroclaw
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43529/ http://psasir.upm.edu.my/id/eprint/43529/1/abstract00.pdf |
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| author | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Sukamat, Hafizah Basri, Mahiran |
| author_facet | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Sukamat, Hafizah Basri, Mahiran |
| author_sort | Oslan, Siti Nurbaya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pichia guilliermondii strain SO isolated from spoiled orange was developed for use as an alternative expression host by using Pichia pastoris as the model of the experiment. This is the first study to report on the capability of P. guilliermondii SO as a host to express thermostable T1 lipase from Geobacillus zalihae. Alcohol oxidase and formaldehyde dehydrogenase promoters were present in the yeast genome. Interestingly, the recombinant yeast [SO/pPICZαB/T1-2 (SO2)] took only 30 h to reach optimal production with minimal methanol induction [1.5% (v/v)] in YPTM medium, as compared to P. pastoris, which took longer to reach its optimal condition. The purification yield of the His-tagged fusion lipase was 68.58%, with specific activity of 194.58 U/mg. The optimum temperature was 65°C at pH 9 in glycine-NaOH buffer, and it was stable up to 70°C in a wide pH range from pH 5 to 12. In conclusion, a newly isolated yeast from spoiled orange has been proven suitable for use as an expression host. |
| first_indexed | 2025-11-15T10:03:21Z |
| format | Article |
| id | upm-43529 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:03:21Z |
| publishDate | 2015 |
| publisher | University of Wroclaw |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-435292016-09-28T04:21:37Z http://psasir.upm.edu.my/id/eprint/43529/ A newly isolated yeast as an expression host for recombinant lipase Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Sukamat, Hafizah Basri, Mahiran Pichia guilliermondii strain SO isolated from spoiled orange was developed for use as an alternative expression host by using Pichia pastoris as the model of the experiment. This is the first study to report on the capability of P. guilliermondii SO as a host to express thermostable T1 lipase from Geobacillus zalihae. Alcohol oxidase and formaldehyde dehydrogenase promoters were present in the yeast genome. Interestingly, the recombinant yeast [SO/pPICZαB/T1-2 (SO2)] took only 30 h to reach optimal production with minimal methanol induction [1.5% (v/v)] in YPTM medium, as compared to P. pastoris, which took longer to reach its optimal condition. The purification yield of the His-tagged fusion lipase was 68.58%, with specific activity of 194.58 U/mg. The optimum temperature was 65°C at pH 9 in glycine-NaOH buffer, and it was stable up to 70°C in a wide pH range from pH 5 to 12. In conclusion, a newly isolated yeast from spoiled orange has been proven suitable for use as an expression host. University of Wroclaw 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43529/1/abstract00.pdf Oslan, Siti Nurbaya and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha and Leow, Thean Chor and Sukamat, Hafizah and Basri, Mahiran (2015) A newly isolated yeast as an expression host for recombinant lipase. Cellular & Molecular Biology Letters, 20 (2). pp. 279-293. ISSN 1425-8153; ESSN: 1689-1392 http://www.degruyter.com/view/j/cmble.2015.20.issue-2/cmble-2015-0015/cmble-2015-0015.xml 10.1515/cmble-2015-0015 |
| spellingShingle | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Sukamat, Hafizah Basri, Mahiran A newly isolated yeast as an expression host for recombinant lipase |
| title | A newly isolated yeast as an expression host for recombinant lipase |
| title_full | A newly isolated yeast as an expression host for recombinant lipase |
| title_fullStr | A newly isolated yeast as an expression host for recombinant lipase |
| title_full_unstemmed | A newly isolated yeast as an expression host for recombinant lipase |
| title_short | A newly isolated yeast as an expression host for recombinant lipase |
| title_sort | newly isolated yeast as an expression host for recombinant lipase |
| url | http://psasir.upm.edu.my/id/eprint/43529/ http://psasir.upm.edu.my/id/eprint/43529/ http://psasir.upm.edu.my/id/eprint/43529/ http://psasir.upm.edu.my/id/eprint/43529/1/abstract00.pdf |