A comparative morphological study between tomato plant and plantlets cv. MT1
Tomato Lycopersicon esculentum is a member of the Solanaceae family. Most of the studies on micropropagation of tomato used stem, hypocotyl, leaf disc and cotyledon as the explant instead of young shoot apical meristem (SAM). Therefore, the suitability of young SAM as explant to regenerate tomato pl...
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| Format: | Article |
| Language: | English |
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International Knowledge Press
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43470/ http://psasir.upm.edu.my/id/eprint/43470/1/A%20comparative%20morphological%20study%20between%20tomato%20plant%20and%20plantlets%20cv.%20MT1.pdf |
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| author | Mahadi, Siti Nuratiqah Nulit, R. Nallappan, Meenakshii |
| author_facet | Mahadi, Siti Nuratiqah Nulit, R. Nallappan, Meenakshii |
| author_sort | Mahadi, Siti Nuratiqah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Tomato Lycopersicon esculentum is a member of the Solanaceae family. Most of the studies on micropropagation of tomato used stem, hypocotyl, leaf disc and cotyledon as the explant instead of young shoot apical meristem (SAM). Therefore, the suitability of young SAM as explant to regenerate tomato plant in vitro was studied. SAM was isolated from 6-days-old germinating tomato seedling and was then inoculated into liquid MS medium supplemented with different concentrations of Indole Acetic Acid (IAA) and Kinetin (KIN) respectively and a combination of both hormones in the ranges of 0.1-0.2 mg/l while semi-solid MS medium alone served as control. Results showed that SAM was able to regenerate to an entire plant in all treatments. However, the best treatment was semi-solid MS medium supplemented with combination of 0.05 mg/L IAA + 0.1 mg/L KIN with an average plant height (4.6 cm), number of leaves (9) and number of roots (7). Our findings also indicated that the morphology of all tomato plantlets were almost similar with tomato plant. Therefore, it was concluded that SAM serves as a suitable explant that can regenerate true plantlets. |
| first_indexed | 2025-11-15T10:03:06Z |
| format | Article |
| id | upm-43470 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:03:06Z |
| publishDate | 2015 |
| publisher | International Knowledge Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-434702018-04-09T04:10:56Z http://psasir.upm.edu.my/id/eprint/43470/ A comparative morphological study between tomato plant and plantlets cv. MT1 Mahadi, Siti Nuratiqah Nulit, R. Nallappan, Meenakshii Tomato Lycopersicon esculentum is a member of the Solanaceae family. Most of the studies on micropropagation of tomato used stem, hypocotyl, leaf disc and cotyledon as the explant instead of young shoot apical meristem (SAM). Therefore, the suitability of young SAM as explant to regenerate tomato plant in vitro was studied. SAM was isolated from 6-days-old germinating tomato seedling and was then inoculated into liquid MS medium supplemented with different concentrations of Indole Acetic Acid (IAA) and Kinetin (KIN) respectively and a combination of both hormones in the ranges of 0.1-0.2 mg/l while semi-solid MS medium alone served as control. Results showed that SAM was able to regenerate to an entire plant in all treatments. However, the best treatment was semi-solid MS medium supplemented with combination of 0.05 mg/L IAA + 0.1 mg/L KIN with an average plant height (4.6 cm), number of leaves (9) and number of roots (7). Our findings also indicated that the morphology of all tomato plantlets were almost similar with tomato plant. Therefore, it was concluded that SAM serves as a suitable explant that can regenerate true plantlets. International Knowledge Press 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43470/1/A%20comparative%20morphological%20study%20between%20tomato%20plant%20and%20plantlets%20cv.%20MT1.pdf Mahadi, Siti Nuratiqah and Nulit, R. and Nallappan, Meenakshii (2015) A comparative morphological study between tomato plant and plantlets cv. MT1. Plant Cell Biotechnology and molecular biology, 16 (3-4). pp. 162-170. ISSN 0972-2025 |
| spellingShingle | Mahadi, Siti Nuratiqah Nulit, R. Nallappan, Meenakshii A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title | A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title_full | A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title_fullStr | A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title_full_unstemmed | A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title_short | A comparative morphological study between tomato plant and plantlets cv. MT1 |
| title_sort | comparative morphological study between tomato plant and plantlets cv. mt1 |
| url | http://psasir.upm.edu.my/id/eprint/43470/ http://psasir.upm.edu.my/id/eprint/43470/1/A%20comparative%20morphological%20study%20between%20tomato%20plant%20and%20plantlets%20cv.%20MT1.pdf |