A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.)
Oil palm chloroplast is maternally inherited, making investigation of the chloroplast diversity an interesting endeavor. This paper describes a method for extracting enriched oil palm chloroplast DNA (cpDNA) done on six palms of different origins from Angola, Nigeria, Ghana, Madagascar and Suriname....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Palm Oil Board
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43465/ http://psasir.upm.edu.my/id/eprint/43465/1/abstract.pdf |
| _version_ | 1848850235855470592 |
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| author | Ho, Carl Miew Ooi, Leslie C. L. Tan, S. G. Ho, Chai Ling Singh, Rajinder |
| author_facet | Ho, Carl Miew Ooi, Leslie C. L. Tan, S. G. Ho, Chai Ling Singh, Rajinder |
| author_sort | Ho, Carl Miew |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Oil palm chloroplast is maternally inherited, making investigation of the chloroplast diversity an interesting endeavor. This paper describes a method for extracting enriched oil palm chloroplast DNA (cpDNA) done on six palms of different origins from Angola, Nigeria, Ghana, Madagascar and Suriname. Restriction enzyme digestion was used to evaluate the successful extraction of the oil palm cpDNA. The use of a mitochondrial DNA specific- universal primer revealed that most of the cpDNA were free from mitochondrial DNA contamination. Three chloroplast- specific universal primers were also used to evaluate the cpDNA. Their amplicons were cloned and sequenced to confirm that the cpDNA was indeed amplified. A search against the public databases further confirmed that the primers amplied sequences of the Elaeis guineensis Jacq. chloroplast genome. Two of them gave consistent amplifications when tested on cpDNA from the Angolan, Nigerian, Ghanian, Madagascan and Suriname palms. |
| first_indexed | 2025-11-15T10:03:04Z |
| format | Article |
| id | upm-43465 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:03:04Z |
| publishDate | 2015 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-434652016-06-24T04:08:38Z http://psasir.upm.edu.my/id/eprint/43465/ A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) Ho, Carl Miew Ooi, Leslie C. L. Tan, S. G. Ho, Chai Ling Singh, Rajinder Oil palm chloroplast is maternally inherited, making investigation of the chloroplast diversity an interesting endeavor. This paper describes a method for extracting enriched oil palm chloroplast DNA (cpDNA) done on six palms of different origins from Angola, Nigeria, Ghana, Madagascar and Suriname. Restriction enzyme digestion was used to evaluate the successful extraction of the oil palm cpDNA. The use of a mitochondrial DNA specific- universal primer revealed that most of the cpDNA were free from mitochondrial DNA contamination. Three chloroplast- specific universal primers were also used to evaluate the cpDNA. Their amplicons were cloned and sequenced to confirm that the cpDNA was indeed amplified. A search against the public databases further confirmed that the primers amplied sequences of the Elaeis guineensis Jacq. chloroplast genome. Two of them gave consistent amplifications when tested on cpDNA from the Angolan, Nigerian, Ghanian, Madagascan and Suriname palms. Malaysian Palm Oil Board 2015-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43465/1/abstract.pdf Ho, Carl Miew and Ooi, Leslie C. L. and Tan, S. G. and Ho, Chai Ling and Singh, Rajinder (2015) A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.). Journal of Oil Palm Research, 27 (4). pp. 306-314. ISSN 1511-2780 |
| spellingShingle | Ho, Carl Miew Ooi, Leslie C. L. Tan, S. G. Ho, Chai Ling Singh, Rajinder A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title | A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title_full | A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title_fullStr | A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title_full_unstemmed | A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title_short | A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) |
| title_sort | chloroplast dna (cpdna) extraction protocol for diversity analysis of oil palm (elaeis sp.) |
| url | http://psasir.upm.edu.my/id/eprint/43465/ http://psasir.upm.edu.my/id/eprint/43465/1/abstract.pdf |