Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study

Haemorrhagic septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of Pasteurella multocida B:2 and E:2 in Asian and African, respectively. It is well known that HS affect mainly the respiratory and digestive tracts. However, involve...

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Main Authors: Marza, Ali Dhiaa, Abdullah, Faez Firdaus Jesse, Ahmed, Ihsan Muneer, Lim, Eric Teik Chung, Ibrahim, Hayder Hamzah, Saad, Mohd Zamri, Omar, Abdul Rahman, Abu Bakar @ Zakaria, Md Zuki, Saharee, Abdul Aziz, Haron, Abdul Wahid, Alwan, Mohammed Jwaid, Mohd Lila, Mohd Azmi
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43327/
http://psasir.upm.edu.my/id/eprint/43327/1/Involvement%20of%20the%20nervous%20system%20following%20experimental%20infection%20with%20Pasteurella%20multocida%20B2%20in%20buffalo%20%28Bubalus%20bubalis%29%20a%20clinicopathological%20study.pdf
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author Marza, Ali Dhiaa
Abdullah, Faez Firdaus Jesse
Ahmed, Ihsan Muneer
Lim, Eric Teik Chung
Ibrahim, Hayder Hamzah
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abdul Wahid
Alwan, Mohammed Jwaid
Mohd Lila, Mohd Azmi
author_facet Marza, Ali Dhiaa
Abdullah, Faez Firdaus Jesse
Ahmed, Ihsan Muneer
Lim, Eric Teik Chung
Ibrahim, Hayder Hamzah
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abdul Wahid
Alwan, Mohammed Jwaid
Mohd Lila, Mohd Azmi
author_sort Marza, Ali Dhiaa
building UPM Institutional Repository
collection Online Access
description Haemorrhagic septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of Pasteurella multocida B:2 and E:2 in Asian and African, respectively. It is well known that HS affect mainly the respiratory and digestive tracts. However, involvement of the nervous system in pathogenesis of HS has been reported in previous studies without details. In this study, nine buffalo calves of 8 months old were distributed into three groups. Animals of Group 1 and 2 were inoculated orally and subcutaneously with 10 ml of 1 × 1012 cfu/ml of P. multocida B:2, respectively, while animals of Group 3 were inoculated orally with 10 ml of phosphate buffer saline as a control. All calves in Group 1 and Group 3 were euthanised after 504 h (21 day) post-infection, while calves in Group 2 had to euthanise after 12 h post-infection as they develop sever clinical signs of HS. Significant differences were found in Group 2 in the mean scores of clinical signs, gross and histopathological changes which mainly affect different anatomic regions of the nervous system. In addition, successful bacterial isolation of P. multocida B:2 were obtained from different sites of the nervous system. On the other hand, less sever, clinical, gross and histopathological changes were found in Group 1. These results provide for the first time strong evidence of involving of the nervous system in pathogenesis of HS, especially in the peracute stage of the disease.
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spelling upm-433272016-11-16T04:51:33Z http://psasir.upm.edu.my/id/eprint/43327/ Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study Marza, Ali Dhiaa Abdullah, Faez Firdaus Jesse Ahmed, Ihsan Muneer Lim, Eric Teik Chung Ibrahim, Hayder Hamzah Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abdul Wahid Alwan, Mohammed Jwaid Mohd Lila, Mohd Azmi Haemorrhagic septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of Pasteurella multocida B:2 and E:2 in Asian and African, respectively. It is well known that HS affect mainly the respiratory and digestive tracts. However, involvement of the nervous system in pathogenesis of HS has been reported in previous studies without details. In this study, nine buffalo calves of 8 months old were distributed into three groups. Animals of Group 1 and 2 were inoculated orally and subcutaneously with 10 ml of 1 × 1012 cfu/ml of P. multocida B:2, respectively, while animals of Group 3 were inoculated orally with 10 ml of phosphate buffer saline as a control. All calves in Group 1 and Group 3 were euthanised after 504 h (21 day) post-infection, while calves in Group 2 had to euthanise after 12 h post-infection as they develop sever clinical signs of HS. Significant differences were found in Group 2 in the mean scores of clinical signs, gross and histopathological changes which mainly affect different anatomic regions of the nervous system. In addition, successful bacterial isolation of P. multocida B:2 were obtained from different sites of the nervous system. On the other hand, less sever, clinical, gross and histopathological changes were found in Group 1. These results provide for the first time strong evidence of involving of the nervous system in pathogenesis of HS, especially in the peracute stage of the disease. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43327/1/Involvement%20of%20the%20nervous%20system%20following%20experimental%20infection%20with%20Pasteurella%20multocida%20B2%20in%20buffalo%20%28Bubalus%20bubalis%29%20a%20clinicopathological%20study.pdf Marza, Ali Dhiaa and Abdullah, Faez Firdaus Jesse and Ahmed, Ihsan Muneer and Lim, Eric Teik Chung and Ibrahim, Hayder Hamzah and Saad, Mohd Zamri and Omar, Abdul Rahman and Abu Bakar @ Zakaria, Md Zuki and Saharee, Abdul Aziz and Haron, Abdul Wahid and Alwan, Mohammed Jwaid and Mohd Lila, Mohd Azmi (2016) Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study. Microbial Pathogenesis, 93. pp. 111-119. ISSN 0882-4010; ESSN: 1096-1208 http://www.sciencedirect.com/science/article/pii/S0882401015301819 10.1016/j.micpath.2016.01.025
spellingShingle Marza, Ali Dhiaa
Abdullah, Faez Firdaus Jesse
Ahmed, Ihsan Muneer
Lim, Eric Teik Chung
Ibrahim, Hayder Hamzah
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abdul Wahid
Alwan, Mohammed Jwaid
Mohd Lila, Mohd Azmi
Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title_full Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title_fullStr Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title_full_unstemmed Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title_short Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): a clinicopathological study
title_sort involvement of the nervous system following experimental infection with pasteurella multocida b:2 in buffalo (bubalus bubalis): a clinicopathological study
url http://psasir.upm.edu.my/id/eprint/43327/
http://psasir.upm.edu.my/id/eprint/43327/
http://psasir.upm.edu.my/id/eprint/43327/
http://psasir.upm.edu.my/id/eprint/43327/1/Involvement%20of%20the%20nervous%20system%20following%20experimental%20infection%20with%20Pasteurella%20multocida%20B2%20in%20buffalo%20%28Bubalus%20bubalis%29%20a%20clinicopathological%20study.pdf