LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination
The purpose of this study was to identify porcine-specific peptide markers from thermally processed meat that could differentiate pork from beef, chevon and chicken meat. In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork were identified using LC–QTOF-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43233/ http://psasir.upm.edu.my/id/eprint/43233/1/LC%E2%80%93QTOF-MS%20identification%20of%20porcine-specific%20peptide%20in%20heat%20treated%20pork%20identifies%20candidate%20markers%20for%20meat%20species%20determination.pdf |
| _version_ | 1848850173794451456 |
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| author | Zainal Abidin, Siti Aimi Sarah Wan Jamaluddin, Wan Noor Faradalila Md Saad, Salwani Ismail, Amin Karsani, Saiful Anuar Sazili, Awis Qurni |
| author_facet | Zainal Abidin, Siti Aimi Sarah Wan Jamaluddin, Wan Noor Faradalila Md Saad, Salwani Ismail, Amin Karsani, Saiful Anuar Sazili, Awis Qurni |
| author_sort | Zainal Abidin, Siti Aimi Sarah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The purpose of this study was to identify porcine-specific peptide markers from thermally processed meat that could differentiate pork from beef, chevon and chicken meat. In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork were identified using LC–QTOF-MS. An MRM method was then established for verification. A thorough investigation of LC–QTOF-MS data showed that only seven porcine-specific peptides were consistently detected. Among these peptides, two were derived from lactate dehydrogenase, one from creatine kinase, and four from serum albumin protein. However, MRM could only detect four peptides (EVTEFAK, LVVITAGAR, FVIER and TVLGNFAAFVQK) that were consistently present in pork samples. In conclusion, meat species determination through a tandem mass spectrometry platform shows high potential in providing scientifically valid and reliable results even at peptide level. Besides, the specificity and selectivity offered by the proteomics approach also provide a robust platform for Halal authentication. |
| first_indexed | 2025-11-15T10:02:05Z |
| format | Article |
| id | upm-43233 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:02:05Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-432332018-05-03T07:24:51Z http://psasir.upm.edu.my/id/eprint/43233/ LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination Zainal Abidin, Siti Aimi Sarah Wan Jamaluddin, Wan Noor Faradalila Md Saad, Salwani Ismail, Amin Karsani, Saiful Anuar Sazili, Awis Qurni The purpose of this study was to identify porcine-specific peptide markers from thermally processed meat that could differentiate pork from beef, chevon and chicken meat. In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork were identified using LC–QTOF-MS. An MRM method was then established for verification. A thorough investigation of LC–QTOF-MS data showed that only seven porcine-specific peptides were consistently detected. Among these peptides, two were derived from lactate dehydrogenase, one from creatine kinase, and four from serum albumin protein. However, MRM could only detect four peptides (EVTEFAK, LVVITAGAR, FVIER and TVLGNFAAFVQK) that were consistently present in pork samples. In conclusion, meat species determination through a tandem mass spectrometry platform shows high potential in providing scientifically valid and reliable results even at peptide level. Besides, the specificity and selectivity offered by the proteomics approach also provide a robust platform for Halal authentication. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43233/1/LC%E2%80%93QTOF-MS%20identification%20of%20porcine-specific%20peptide%20in%20heat%20treated%20pork%20identifies%20candidate%20markers%20for%20meat%20species%20determination.pdf Zainal Abidin, Siti Aimi Sarah and Wan Jamaluddin, Wan Noor Faradalila and Md Saad, Salwani and Ismail, Amin and Karsani, Saiful Anuar and Sazili, Awis Qurni (2016) LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination. Food Chemistry, 199. pp. 157-164. ISSN 0308-8146; ESSN: 1873-7072 http://www.sciencedirect.com/science/article/pii/S0308814615302661 10.1016/j.foodchem.2015.11.121 |
| spellingShingle | Zainal Abidin, Siti Aimi Sarah Wan Jamaluddin, Wan Noor Faradalila Md Saad, Salwani Ismail, Amin Karsani, Saiful Anuar Sazili, Awis Qurni LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title_full | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title_fullStr | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title_full_unstemmed | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title_short | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| title_sort | lc–qtof-ms identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination |
| url | http://psasir.upm.edu.my/id/eprint/43233/ http://psasir.upm.edu.my/id/eprint/43233/ http://psasir.upm.edu.my/id/eprint/43233/ http://psasir.upm.edu.my/id/eprint/43233/1/LC%E2%80%93QTOF-MS%20identification%20of%20porcine-specific%20peptide%20in%20heat%20treated%20pork%20identifies%20candidate%20markers%20for%20meat%20species%20determination.pdf |