Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV)
This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed wit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43204/ http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf |
| _version_ | 1848850165908111360 |
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| author | Uda, Muhammad Nur Aiman Che Man, Hasfalina Abd Aziz, Samsuzana Ishak, Zamri Bunawan, Siti Noraini Wahid, Nur Sabrina Hashim, Uda |
| author_facet | Uda, Muhammad Nur Aiman Che Man, Hasfalina Abd Aziz, Samsuzana Ishak, Zamri Bunawan, Siti Noraini Wahid, Nur Sabrina Hashim, Uda |
| author_sort | Uda, Muhammad Nur Aiman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively. |
| first_indexed | 2025-11-15T10:01:58Z |
| format | Article |
| id | upm-43204 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:01:58Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-432042017-09-27T08:56:07Z http://psasir.upm.edu.my/id/eprint/43204/ Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV) Uda, Muhammad Nur Aiman Che Man, Hasfalina Abd Aziz, Samsuzana Ishak, Zamri Bunawan, Siti Noraini Wahid, Nur Sabrina Hashim, Uda This paper studies two different virus purification methods for rice tungro bacilliform virus. In the first method, infected samples were extracted using liquid nitrogen with mixture of 10% Cellulases whereas the second method used alternate spinning of the infected samples at high and low speed with 5% Cellulases. Viral particles from both methods were examined by transmission electron microscopy (TEM) and rod-shaped with rounded ends was only seen on samples that were extracted with method 2. The estimated purity and concentration of method 2 is higher compare to method 1 which are 1.362 and 1.608 mg/ml and 0.958 and 0.299 mg/ml respectively. Elsevier 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf Uda, Muhammad Nur Aiman and Che Man, Hasfalina and Abd Aziz, Samsuzana and Ishak, Zamri and Bunawan, Siti Noraini and Wahid, Nur Sabrina and Hashim, Uda (2014) Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV). Agriculture and Agricultural Science Procedia, 2. pp. 107-112. ISSN 2210-7843 http://www.sciencedirect.com/science/article/pii/S2210784314000175 10.1016/j.aaspro.2014.11.016 |
| spellingShingle | Uda, Muhammad Nur Aiman Che Man, Hasfalina Abd Aziz, Samsuzana Ishak, Zamri Bunawan, Siti Noraini Wahid, Nur Sabrina Hashim, Uda Comparison study of two different isolation and purification method for rice tungro bacilliform virus (RTBV) |
| title | Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV) |
| title_full | Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV) |
| title_fullStr | Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV) |
| title_full_unstemmed | Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV) |
| title_short | Comparison study of two different isolation and purification
method for rice tungro bacilliform virus (RTBV) |
| title_sort | comparison study of two different isolation and purification
method for rice tungro bacilliform virus (rtbv) |
| url | http://psasir.upm.edu.my/id/eprint/43204/ http://psasir.upm.edu.my/id/eprint/43204/ http://psasir.upm.edu.my/id/eprint/43204/ http://psasir.upm.edu.my/id/eprint/43204/1/Comparison%20Study%20of%20Two%20Different%20Isolation%20and%20Purification.pdf |