Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet

The Zingiberaceae family is found in tropical and subtropical areas, with approximately 161 species from 18 genera of this family found in Peninsular Malaysia. Zingiber zerumbet (L.) Smith tree belonging to this family is an edible ginger, originating in South-East Asia, and has been cultivated for...

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Main Authors: Rahman, Heshu Sulaiman, Othman, Hemn Hassan, Abdullah, R., Chartrand, Max Stanley, Allaudin, Zeenathul Nazariah, Ajdari, Zahra, Hosseinpour, Mahnaz, Abdul Samad, Nozlena
Format: Conference or Workshop Item
Language:English
Published: UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience 2014
Online Access:http://psasir.upm.edu.my/id/eprint/42514/
http://psasir.upm.edu.my/id/eprint/42514/1/ABSTRACT%20CAC%202014_2%20medic%207.pdf
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author Rahman, Heshu Sulaiman
Othman, Hemn Hassan
Abdullah, R.
Chartrand, Max Stanley
Allaudin, Zeenathul Nazariah
Ajdari, Zahra
Hosseinpour, Mahnaz
Abdul Samad, Nozlena
author_facet Rahman, Heshu Sulaiman
Othman, Hemn Hassan
Abdullah, R.
Chartrand, Max Stanley
Allaudin, Zeenathul Nazariah
Ajdari, Zahra
Hosseinpour, Mahnaz
Abdul Samad, Nozlena
author_sort Rahman, Heshu Sulaiman
building UPM Institutional Repository
collection Online Access
description The Zingiberaceae family is found in tropical and subtropical areas, with approximately 161 species from 18 genera of this family found in Peninsular Malaysia. Zingiber zerumbet (L.) Smith tree belonging to this family is an edible ginger, originating in South-East Asia, and has been cultivated for thousands of years as a spice and for medical purposes. The aim of this study is to isolate the active principle from extracted essential oil of fresh Zingiber Zerumbet rhizomes by steam-hydrodistillation method. In addition, to determine the purity of this active compound using validated reverse phase high performance liquid chromatography (RP-HPLC). Moreover, the antiproliferative effects of this active principal on various human cancerous and noncancerous cell lines at concentrations of 1 to 100 μg/mL were quantified by MTT assay. As a result, colorless zerumbone (ZER) crystals about 1.3 g/kg as an active principal were extracted from the essential oil of fresh Z. Zerumbet rhizomes. The purity of ZER crystals were shown to be (99.96%). Simultaneously, ZER exhibited significant (P < 0.05) inhibitory effects towards various human cancerous cell lines, while not affected noncancerous cell lines. In conclusion, ZER is suggested to be further developed into a safe therapeutic compound for the treatment of various human cancers.
first_indexed 2025-11-15T09:59:04Z
format Conference or Workshop Item
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T09:59:04Z
publishDate 2014
publisher UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience
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spelling upm-425142016-04-26T02:37:17Z http://psasir.upm.edu.my/id/eprint/42514/ Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet Rahman, Heshu Sulaiman Othman, Hemn Hassan Abdullah, R. Chartrand, Max Stanley Allaudin, Zeenathul Nazariah Ajdari, Zahra Hosseinpour, Mahnaz Abdul Samad, Nozlena The Zingiberaceae family is found in tropical and subtropical areas, with approximately 161 species from 18 genera of this family found in Peninsular Malaysia. Zingiber zerumbet (L.) Smith tree belonging to this family is an edible ginger, originating in South-East Asia, and has been cultivated for thousands of years as a spice and for medical purposes. The aim of this study is to isolate the active principle from extracted essential oil of fresh Zingiber Zerumbet rhizomes by steam-hydrodistillation method. In addition, to determine the purity of this active compound using validated reverse phase high performance liquid chromatography (RP-HPLC). Moreover, the antiproliferative effects of this active principal on various human cancerous and noncancerous cell lines at concentrations of 1 to 100 μg/mL were quantified by MTT assay. As a result, colorless zerumbone (ZER) crystals about 1.3 g/kg as an active principal were extracted from the essential oil of fresh Z. Zerumbet rhizomes. The purity of ZER crystals were shown to be (99.96%). Simultaneously, ZER exhibited significant (P < 0.05) inhibitory effects towards various human cancerous cell lines, while not affected noncancerous cell lines. In conclusion, ZER is suggested to be further developed into a safe therapeutic compound for the treatment of various human cancers. UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience 2014 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42514/1/ABSTRACT%20CAC%202014_2%20medic%207.pdf Rahman, Heshu Sulaiman and Othman, Hemn Hassan and Abdullah, R. and Chartrand, Max Stanley and Allaudin, Zeenathul Nazariah and Ajdari, Zahra and Hosseinpour, Mahnaz and Abdul Samad, Nozlena (2014) Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet. In: Scientific Cancer Research Poster Competition in conjunction with Cancer Awareness Carnival 2014, 10 May 2014, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. .
spellingShingle Rahman, Heshu Sulaiman
Othman, Hemn Hassan
Abdullah, R.
Chartrand, Max Stanley
Allaudin, Zeenathul Nazariah
Ajdari, Zahra
Hosseinpour, Mahnaz
Abdul Samad, Nozlena
Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title_full Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title_fullStr Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title_full_unstemmed Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title_short Isolation, purification and evaluation of anticancer principle from Zingiber zerumbet
title_sort isolation, purification and evaluation of anticancer principle from zingiber zerumbet
url http://psasir.upm.edu.my/id/eprint/42514/
http://psasir.upm.edu.my/id/eprint/42514/1/ABSTRACT%20CAC%202014_2%20medic%207.pdf